A preparation method and application of tea nano-dots
A technology of nano-dots and tea leaves, applied in the direction of nano-technology, nano-technology, nano-medicine, etc., to achieve the effect of enhancing stability, solving the problem of bacterial drug resistance, and enhancing antibacterial activity
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Embodiment 1
[0091] A preparation method of tea nano-dots, the method comprises the following steps:
[0092] 1) pulverizing: get the dry tea leaves of black tea, cut them into pieces with scissors to a particle less than 0.5cm, and grind the cut tea leaves into uniform tea powder through mortar grinding, and the particle diameter of the tea powder is 0.8mm;
[0093]2) Extraction: mix described tea powder 5g with 75g of water, place in the polytetrafluoroethylene liner of the autoclave and carry out hydrothermal reaction, seal the polytetrafluoroethylene liner of the autoclave and place it in. In the muffle furnace, the muffle furnace was heated to 80 °C at a rate of 2 °C / min and kept for 6 hours. The reaction pressure in the polytetrafluoroethylene liner of the autoclave was 1.6 standard atmospheres. After the reaction was completed, it was naturally cooled to room temperature to obtain a crude extract;
[0094] 3) separation: the crude extract is filtered with a filter cloth (150 mesh) ...
Embodiment 2
[0126] Taking Black TNDs as the research object, the construction of Black TNDs and the molecular dynamics (MD) simulation of the transmembrane process of Black TNDs were carried out, including the following parts:
[0127] 1. Obtain the top three Black TNDs models through molecular docking, and then evaluate their stability through MD simulation (100ns)
[0128] Construction of Black TNDs: Black TNDs were constructed by all-atom molecular dynamics (MD) simulations. First, three-dimensional structures of eight molecules (CA, GC, CG, GCG, EC, EGC, ECG, and EGCG) were constructed, and they were assigned AD atom types individually using AutodockTools, which were then hydrogenated, charged, and finally saved as ". pdb" type file. Using HEX8.0 program to dock the constructed eight molecules in turn, calculate the corresponding lowest energy mode among the eight molecules between each other, and select it as the input file for the next molecular docking. Then, the possible interac...
Embodiment 3
[0137] Proteomic analysis of MRSA processed by Black TNDs, including the following sections:
[0138] 1. Tandem mass tagging (TMT) technology is used for quantitative proteomic analysis: MRSA bacteria without and after BlackTNDs treatment were collected as bacterial samples for proteomic analysis, the bacterial samples were washed 3 times, then SDT lysate was added, and sonicated The treatment accelerated cell lysis, then boiled for 15 minutes, and after centrifugation at 14,000g for 40 minutes at 4°C, the corresponding supernatant was quantified with a BCA protein assay kit (Bio-Rad, USA). The samples were stored at -80 before use. ℃ conditions. Next, protein samples were isolated and visualized by SDS-PAGE separation. The filter-assisted sample preparation (FASP) method is performed during the enzymatic digestion of proteins. Additionally, the peptide mixture for each sample was labeled using TMT 6 / 10plex isobaric labeling reagent (Thermo), and the TMT-labeled samples were...
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