A kind of magnetic nanoparticle wrapped by erythrocyte membrane and its preparation method and application

A technology of magnetic nanoparticles and red blood cell membranes, which is applied in the field of nano-biomedical materials, can solve the problems of unfavorable metabolism, hidden dangers of toxicity, and easy accumulation of particles, and achieve good biocompatibility, long-term blood circulation, increased deformability, and preparation simple effect

Active Publication Date: 2022-05-20
CENT SOUTH UNIV
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  • Abstract
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Problems solved by technology

Fe 3 o 4 The disadvantage is that the magnetic effect makes the particles easy to aggregate and precipitate. Too large particles are not conducive to human metabolism, and are easy to accumulate in the liver, kidneys, and lymph nodes, causing side effects that the human body cannot tolerate
Although the current technology is in the process of preparing magnetic nanoparticles, Fe 3 o 4 Surface modification can enhance its suspension, but in order to achieve the purpose of reducing particle size, toxic surfactants such as sodium dodecylbenzenesulfonate, organic solvent tetrahydrofuran, DMSO, etc. are often used, and it is often unavoidable The recognition and phagocytosis of immune cells, although the produced particles have excellent biological effects, but there are also hidden dangers of toxicity and the risk of being phagocytized by immune cells

Method used

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  • A kind of magnetic nanoparticle wrapped by erythrocyte membrane and its preparation method and application
  • A kind of magnetic nanoparticle wrapped by erythrocyte membrane and its preparation method and application
  • A kind of magnetic nanoparticle wrapped by erythrocyte membrane and its preparation method and application

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Embodiment 1

[0070] (1) PEG-Fe 3 o 4 preparation:

[0071] 2.00g (0.01mol) FeCl 2 4H 2 O, 4.725g (0.02mol) FeCl 3 ·6H 2 O Add 20 mL of distilled water, then add 6 g of PEG6000, and sonicate for 10 min (the power of the ultrasonic cell disruptor is 800 W, the frequency is 20 kHz) until dissolved. Stir at 1400r / min under a nitrogen atmosphere, heat up to 60°C, and react for 30min. Then add dropwise a solution containing 6g of PEG6000, the volume of which is about 1 / 5 of the reaction solution, transfer the reaction solution to a polytetrafluoroethylene reactor, set the temperature at 160°C, raise the temperature for 1 hour, and react for 5 hours. get Fe 3 o 4 For nanoparticle precipitation, first wash the precipitate with pure water and ethanol, repeat several times, and dry in a vacuum oven at 60°C for 10 hours to finally obtain PEG-Fe 3 o 4 nanoparticles.

[0072] (2) Extraction and targeted modification of erythrocyte membrane fragments:

[0073] Put the sheep blood into a cent...

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Abstract

The invention relates to a magnetic nanoparticle wrapped in red blood cell membrane and its preparation method and application, specifically PEG-Fe prepared by hydrothermal method 3 o 4 Extrusion and encapsulation of targeted modified erythrocyte membrane fragments to prepare nanoparticle erythrocyte membrane-PEG-Fe with immune evasion and sensitive magnetic responsiveness 3 o 4 , can target and adsorb cancer cells sensitive to folic acid, so that it can be controlled by a magnetic field or killed by light and heat stimulation. Erythrocyte membrane‑PEG‑Fe 3 o 4 It can be effectively targeted and adsorbed to the membrane of folic acid-sensitive cancer cells, endowing cancer with magnetic responsiveness, which is conducive to the control of movement by magnetic field and killing by light and heat stimulation. Therefore, it has potential application prospects in targeted therapy and industrial promotion.

Description

technical field [0001] The invention belongs to the field of nano-biomedical materials, and relates to a preparation method of magnetic nanoparticles wrapped by red blood cell membranes, a product and an application thereof. Background technique [0002] Cancer is a major disease that threatens human life. Conventional chemotherapy, radiotherapy and surgery have limited effects and high side effects. The development of new technologies is urgently needed to meet the requirements of the cancer market. Targeting magnetic nanoparticles is a current research focus, and magnetic Fe 3 o 4 It has unique magnetic properties and thermal conductivity, and has good biocompatibility. It has been widely concerned in cancer treatment. It can stimulate a large amount of heat with a small amount of infrared light energy. It is a good material for photothermal therapy, and its magnetic properties have It is beneficial to impart magnetic responsiveness to cancer cells, and is convenient for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/46A61K47/60A61K47/10A61K47/12A61K47/24A61K47/22A61K47/26A61P35/00A61K49/00
CPCA61K41/0052A61K9/5123A61K9/5146A61K9/5176A61P35/00A61K49/0041A61K49/0054A61K49/0043A61K49/0093
Inventor 彭逸舟段金娣杨艳婧钟世安
Owner CENT SOUTH UNIV
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