Method for improving methane yield of mixed bacteria system by coupling electric signals and chemical signals

A signal coupling and electrical signal technology, applied in the energy field, can solve problems such as low methane synthesis efficiency, inappropriate bacterial metabolism, and difficulty in mass and heat transfer, so as to improve methane synthesis efficiency, promote mutual benefit and symbiosis, and have wide application foreground effect

Pending Publication Date: 2021-10-29
NANJING UNIV OF TECH
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are many bacterial groups involved in the reaction process, different bacterial groups are interdependent and mutually restrictive, forming a complex interaction network, and there are many biochemical reactions in the anaerobic digestion process, involving solid, liquid and gas multiphase, The metabolic process is complex, and mass and heat transfer are difficult, so the substrate utilization rate is low, and the methane synthesis efficiency has not been high
At present, most studies are devoted to improving the anaerobic digestion methane production by optimizing the operating conditions of the anaerobic fermentation system, adding functional material mediation and bioaugmentation, etc., but the effect is not obvious, and the organic A series of key problems such as low substrate degradation utilization rate, long start-up cycle, and inappropriate bacterial metabolism

Method used

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  • Method for improving methane yield of mixed bacteria system by coupling electric signals and chemical signals
  • Method for improving methane yield of mixed bacteria system by coupling electric signals and chemical signals
  • Method for improving methane yield of mixed bacteria system by coupling electric signals and chemical signals

Examples

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Effect test

Embodiment 1

[0052] This embodiment illustrates that when the anaerobic fermentation is regulated by electrical signals, the present invention adopts the enriched electroactive biofilm anode electrochemical regulation (abbreviated as 0.8V+ biofilm) and the non-enriched electroactive biofilm anode electrochemical regulation ( Abbreviated as 0.8V) on anaerobic fermentation.

[0053] build as figure 1 In the anaerobic fermentation device shown, the anaerobic fermentation system is externally connected to an external DC power supply (0.8V), and an anode electrode and a cathode electrode are respectively set up. The electrodes are connected to the external power supply through a titanium wire. The cathode and anode electrodes are carbon felts, and the external resistance is 10Ω.

[0054] Anode electroactive biofilm enrichment: At 38±2°C, double-chamber MFC was used to enrich electrogenic bacteria at the anode. When the output voltage value of the MFC tended to be stable, the biofilm domesticat...

Embodiment 2

[0059] This example illustrates the influence on anaerobic fermentation under the regulation of different voltages in the present invention when electrical signals are used to regulate anaerobic fermentation.

[0060] build as figure 1 In the anaerobic fermentation device shown, the anaerobic fermentation system is externally connected to an external DC power supply, and the DC power supply voltage is set to 0.5V, 0.8V, and 1.0V, respectively, and an anode electrode and a cathode electrode are respectively set. The electrodes are connected to the external power supply through a titanium wire. Connection, the positive and negative electrodes are carbon felts.

[0061] The enrichment of the fermentation inoculum was as described in Example 1.

[0062] Add the inoculum of domesticated bacteria into the fermentation system according to 30% of the total system mass, and the fermentation substrate is straw and cow dung in a ratio of 1:3 into the system, and then add the carbon felt...

Embodiment 3

[0065] This example illustrates that when chemical signals are used to regulate anaerobic fermentation, the present invention uses different types of chemical signal molecules, different concentrations of chemical signal molecules, and the influence of chemical signal molecules on anaerobic fermentation under the regulation of adding chemical signal molecules in different fermentation stages.

[0066] The enrichment of the fermentation inoculum was as described in Example 1.

[0067] The acclimatized bacterial source inoculum was added to the fermentation system according to 30% of the total system mass, and the fermentation substrate was straw and cow dung and added to the system at a ratio of 1:3. Anaerobic fermentation was carried out for 30 days under the conditions of 38±2°C and a fermentation system volume of 500mL.

[0068] 10 μM of different types of chemical signaling molecules, namely C6-HSL, C7-HSL, and C12-HSL, were added to the system at 0d, and the traditional fe...

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Abstract

The invention provides a method for improving the methane yield of a mixed bacteria system by coupling electric signals and chemical signals. The method comprises the following steps: domesticating a double-chamber microbial fuel cell (MFC) at an anode to obtain an electrode enriched with an electroactive biological membrane; then constructing a dual-electrode system in an anaerobic fermentation device, namely inserting a domesticated electroactive biological membrane electrode as the anode, taking an electrode without a biological membrane as a cathode, and externally applying direct-current voltage to two ends of the dual electrodes; and in an anaerobic fermentation system, directionally enriching microbial florae with different functions by adding chemical signal molecules. The method provided by the invention is used for methane production in the mixed bacteria system, can enhance information exchange among florae, is beneficial to mutualism among the florae, realizes metabolism matching of microbial action stages of fermentation acid production, hydrogen production, acetic acid production and methane production, and finally improves the methane synthesis efficiency. The method provides a new technical approach for resourceful treatment of organic wastes, and has a wide application prospect in the aspects of environmental protection and low-quality biomass utilization.

Description

technical field [0001] The invention relates to the fields of energy, environment and electrochemistry, in particular to a method for increasing methane production in a mixed bacteria system by coupling electrical signals and chemical signals. Background technique [0002] With the rapid development of economy and technology in today's society, human beings are increasingly dependent on energy, and the damage to nature is also increasing. With the gradual depletion of fossil energy and the intensification of the greenhouse effect, the impact of energy shortage and environmental pollution on the world has become increasingly prominent. Livestock and poultry manure and crop straw are two major biomass resources in my country. The annual production of livestock and poultry manure is about 2 billion tons (wet weight), and the output of crop straw can reach more than 800 million tons. The resources are abundant and widely distributed. However, if it is not used effectively, it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12M1/107C12M1/42C12M1/24C02F11/04C25B3/03C25B11/043
CPCC12P5/023C12M21/04C12M23/08C12M23/36C12M35/02C02F11/04C25B3/03C25B11/043Y02E50/30
Inventor 吴夏芫张丽娜贾红华
Owner NANJING UNIV OF TECH
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