Method for improving methane yield of mixed bacteria system by coupling electric signals and chemical signals
A signal coupling and electrical signal technology, applied in the energy field, can solve problems such as low methane synthesis efficiency, inappropriate bacterial metabolism, and difficulty in mass and heat transfer, so as to improve methane synthesis efficiency, promote mutual benefit and symbiosis, and have wide application foreground effect
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Embodiment 1
[0052] This embodiment illustrates that when the anaerobic fermentation is regulated by electrical signals, the present invention adopts the enriched electroactive biofilm anode electrochemical regulation (abbreviated as 0.8V+ biofilm) and the non-enriched electroactive biofilm anode electrochemical regulation ( Abbreviated as 0.8V) on anaerobic fermentation.
[0053] build as figure 1 In the anaerobic fermentation device shown, the anaerobic fermentation system is externally connected to an external DC power supply (0.8V), and an anode electrode and a cathode electrode are respectively set up. The electrodes are connected to the external power supply through a titanium wire. The cathode and anode electrodes are carbon felts, and the external resistance is 10Ω.
[0054] Anode electroactive biofilm enrichment: At 38±2°C, double-chamber MFC was used to enrich electrogenic bacteria at the anode. When the output voltage value of the MFC tended to be stable, the biofilm domesticat...
Embodiment 2
[0059] This example illustrates the influence on anaerobic fermentation under the regulation of different voltages in the present invention when electrical signals are used to regulate anaerobic fermentation.
[0060] build as figure 1 In the anaerobic fermentation device shown, the anaerobic fermentation system is externally connected to an external DC power supply, and the DC power supply voltage is set to 0.5V, 0.8V, and 1.0V, respectively, and an anode electrode and a cathode electrode are respectively set. The electrodes are connected to the external power supply through a titanium wire. Connection, the positive and negative electrodes are carbon felts.
[0061] The enrichment of the fermentation inoculum was as described in Example 1.
[0062] Add the inoculum of domesticated bacteria into the fermentation system according to 30% of the total system mass, and the fermentation substrate is straw and cow dung in a ratio of 1:3 into the system, and then add the carbon felt...
Embodiment 3
[0065] This example illustrates that when chemical signals are used to regulate anaerobic fermentation, the present invention uses different types of chemical signal molecules, different concentrations of chemical signal molecules, and the influence of chemical signal molecules on anaerobic fermentation under the regulation of adding chemical signal molecules in different fermentation stages.
[0066] The enrichment of the fermentation inoculum was as described in Example 1.
[0067] The acclimatized bacterial source inoculum was added to the fermentation system according to 30% of the total system mass, and the fermentation substrate was straw and cow dung and added to the system at a ratio of 1:3. Anaerobic fermentation was carried out for 30 days under the conditions of 38±2°C and a fermentation system volume of 500mL.
[0068] 10 μM of different types of chemical signaling molecules, namely C6-HSL, C7-HSL, and C12-HSL, were added to the system at 0d, and the traditional fe...
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