Bacillus and application thereof

A technology of Bacillus coagulans and Bacillus coagulans, applied in the field of microorganisms, can solve the problems that the number of viable bacteria of Clostridium butyricum Bacillus coagulans needs to be increased, the problem of contamination is not easy to solve, and the cooling cost is high, so as to enhance animal immunity and resist The effect of strong reversibility and improved palatability

Active Publication Date: 2021-11-16
HENAN JINBAIHE BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent with the patent number CN202010482530.X that we applied for earlier discloses a liquid mixed fermentation method of Clostridium butyricum and Bacillus coagulans. The content protected by this patent has the following defects: 1) Clostridium butyricum obtained by the mixed fermentation method The number of viable bacteria and Bacillus coagulans still needs to be improved; 2) The fermentation temperature of mixed fermentation is 33°C-37°C, and the cooling cost is high; 3) The problem of bacterial contamination is not easy to solve

Method used

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  • Bacillus and application thereof
  • Bacillus and application thereof
  • Bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The screening of embodiment 1 bacillus coagulans

[0042] 1. The medium (liquid) and its formula (g / L) used for strain screening are as follows:

[0043] Enrichment medium (nutrient broth medium): peptone 10, beef extract 3, sodium chloride 5, pH 7.0,

[0044] Calcium carbonate identification plate medium: glucose 5, peptone 5, yeast extract powder 1, calcium carbonate 0.3, agar powder 10, pH6.8~7.0.

[0045] Lactic acid bacteria proliferation medium (YPG): glucose 5, peptone 5, yeast extract 1, calcium chloride 0.1, pH 6.8-7.0.

[0046] Coagulant spore activation medium (1 / 2YPD medium): glucose 10, yeast extract 5, tryptone 10, pH 6.8-7.0.

[0047] Bacillus coagulans count medium (1 / 2YPD solid medium): glucose 10, yeast extract 5, tryptone 10, distilled water 1000mL, agar powder 15, pH 6.8.

[0048] Bacillus coagulans slant medium: beef extract 5, tryptone 10, sodium chloride 5, pH 7.0±0.1.

[0049] Amylase medium: peptone 10, beef extract 5, sodium chloride 5, sta...

Embodiment 2

[0059] Stress resistance and enzyme production performance research of embodiment 2 bacterial strain

[0060] 1. Simulated gastric juice tolerance study

[0061] Centrifuge the activated candidate strain bacterial liquid at 4000r / min for 10min to collect bacterial precipitates, add the collected bacterial cells to the simulated gastric juice, and culture them statically at 37°C, take samples at 0, 60min, 120min, and 180min to detect the activity Bacteria count and calculate survival rate, the results are shown in Table 2:

[0062] Table 2 Survival rate (%) of different strains after artificial gastric juice resistance treatment

[0063]

[0064] From the results in Table 2, it can be seen that the activity of all strains was lost after being treated with simulated artificial gastric juice, and the survival rate of strain ZC2-1 was the highest after being treated for 120 minutes.

[0065] 2. Research on bile salt tolerance

[0066] Bile salts in the small intestine of ani...

Embodiment 3

[0078] Embodiment 3 strain identification

[0079] In this example, 4 strains ZC2-1, ZA-1, ZB-1 and ZC-9 with good enzyme production, gastric acid resistance, bile salt resistance and heat resistance were identified through morphological and phylogenetic analysis.

[0080] 1. Morphological identification

[0081] The above four strains were activated and inoculated on the YPD solid medium plate, and cultured at 37°C for 24 hours to observe the colony morphology. Then take an appropriate amount of bacterial liquid for methylene blue staining and microscopic examination. The result is as image 3 shown. Depend on image 3 It can be seen that the bacterial strain ZC2-1 forms a white opaque circular colony, and there is a small white protrusion in the middle of the colony ( image 3 a), the thallus is in the shape of a slender rod with a length of 2.5-4.0 μm and a width of 0.4-0.8 μm ( image 3 e). Strains ZA-1 and ZC-9 both formed light milky white round small colonies wit...

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Abstract

The invention relates to a bacillus and application thereof. The bacillus coagulans is screened from intestinal contents of healthy piglets, the preservation number of the bacillus coagulans is CGMCC 22951, and the bacillus coagulans can produce acid, amylase and protease, is high in gastric acid resistance and cholate resistance and good in thermal stability, and can grow rapidly at the intestinal temperature of -39 DEG C of livestock and poultry. The invention further provides an application of the bacillus in facultative co-fermentation with clostridium butyricum. Through a high-temperature (39 DEG C) co-fermentation system, the cooling cost is reduced on one hand, and the growth of impurities are effectively inhibited on the other hand, so that the fermentation process is simplified; the newly screened strains grow faster under the facultative oxygen condition, so that the viable bacterium concentration and the spore concentration of the clostridium butyricum in a co-fermentation system are higher, and a new energy-saving and environment-friendly mode is provided for production of the clostridium butyricum.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus strain and its application. Background technique [0002] Antibiotics, as feed additives, made a positive contribution to intensive farming in the 1980s. However, in recent years, the problem of antibiotic abuse has become prominent. Long-term abuse of antibiotics has brought problems such as breeding safety, food safety, environmental safety, and biological safety. On the one hand, it leads to the increase of drug-resistant bacteria and drug-resistant genes, affecting the ecological environment; on the other hand, antibiotics will spread from animals to humans through the food chain. Long-term consumption of "resistant foods" will gradually increase the resistance of bacteria, resulting in an antibiotic crisis. Once a patient is infected by a drug-resistant pathogen, there may be no cure for it. Therefore, it is imperative to disable (restrict) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A61K35/742A61P1/00A61P37/04C12R1/07C12R1/145
CPCC12N1/20A23K10/18A61K35/742A61P1/00A61P37/04
Inventor 李永红李克克冯利菲王蕴李旋李光华韩亚坤
Owner HENAN JINBAIHE BIOTECH CO LTD
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