Application of soluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis

A technology for ulcerative colitis and functional food, which is applied in the application field of medicine and functional food, can solve the problems of large side effects, etc., and achieve the effects of increased absorption, high safety and safe use

Pending Publication Date: 2021-12-03
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the incidence of IBD has increased significantly worldwide. Inflammatory drugs, immunosuppressants, and biological drugs can all be used in the treatment of IBD, but they have relatively large side effects

Method used

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  • Application of soluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis
  • Application of soluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis
  • Application of soluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The extraction method of soluble dietary fiber among the present invention comprises the following steps:

[0045] (1) Add 5% NaCl solution to the kelp residue according to the ratio of solid to liquid ratio of 1:30, and heat at 90°C for 7 h for decalcification treatment;

[0046] (2) Centrifuge to remove the supernatant, add an appropriate amount of pure water to the precipitate and boil for 30 minutes;

[0047] (3) Add 5 mol / L NaOH solution to the precipitate in step (2) according to the ratio of solid to liquid 1:30, adjust the pH to 13, and heat at 90°C for 6 h;

[0048] (4) Centrifuge and collect the precipitate as SDF;

[0049] (5) SDF is adjusted to neutral pH with HCl, concentrated by rotary evaporation, dialyzed in a 1500 Da dialysis bag for 10-12 h, placed in a -80°C refrigerator for 12 h, and then freeze-dried for 36 h to obtain dry soluble dietary fiber .

Embodiment 2

[0050] Example 2: Identification of the basic structure of kelp soluble dietary fiber (SDF)

[0051] 1. Molecular weight distribution and monosaccharide composition of soluble kelp dietary fiber

[0052] The molecular weight distribution and monosaccharide composition of the soluble kelp dietary fiber prepared in Example 1 were investigated using GPC and HPLC respectively.

[0053] Complete acid hydrolysis: Take an appropriate amount of sample in a hydrolysis tube, add 1 mL of 4mol / L TFA (trifluoroacetic acid), and hydrolyze in an oven at 120°C for 2 h. After taking it out, dry it with nitrogen gas; derivatization reaction: add 1 mL 0.5 mol / L PMP-methanol solution and 0.5 mL 0.3 mol / L NaOH solution to the dried sample, 70 °C water bath for 60 min, cool, add 0.5 mL 0.3 After adding mol / L HCl solution, add 0.5 mL chloroform, oscillate and shake well, then let it stand for 20 min, discard the lower layer, extract three times, and take the water layer to pass through the membrane...

Embodiment 3

[0067] Select C57BL / 6 mice (male), 7-8 weeks old, body weight 25-28g, rearing temperature 20-24°C, humidity 40%-60%, free to drink water and eat during the period. The experimental grouping was as follows: 72 male C57BL / 6 mice were adaptively fed for 7 days and then randomly divided into 9 groups according to body weight, with 12 mice in each group, which were normal group (NC), model group (MC), positive drug group (PC), Low, medium and high dose SDF groups (L-SDF, M-SDF, H-SDF). At the end of adaptive feeding, the test substance was gavaged. The mice in each group were gavaged with the test substance every day at 9:00 am for 7 days. On the 8th day, dextran sodium sulfate (DSS) was used to establish the ulcerative colitis model. After the last meal, on the 14th day, the mice were fasted without food and water overnight. The next day, the mice were sacrificed by dislodging the cervical spine after taking blood from the eye socket. Intervention plan See Table 2. During the i...

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Abstract

The invention discloses an application of soluble dietary fibers in kelp in preparation of a medicine and functional food for improving ulcerative colitis. According to the invention, soluble dietary fibers are extracted from the kelp residues, an ulcerative colitis mouse model is constructed, and it is verified for the first time that the soluble dietary fibers can inhibit weight loss and disease activity indexes of mice; inhibit colon disease and shortening degree; improve a level of oxidative stress in the colon; balance a level of inflammatory factors in a body; up-regulate expression of intestinal tight junction protein; restore diversity and abundance of intestinal flora; and increase production of short-chain fatty acids such as butyric acid, acetic acid, and propionic acid. The soluble dietary fibers in kelp belong to natural active substances, are safe and free of toxic and side effects, can be used for treating enteritis and other gastrointestinal diseases based on diet intervention, avoid adverse reactions of traditional drug treatment, and have good application prospects.

Description

technical field [0001] The invention belongs to the field of food health care, and in particular relates to the application of soluble dietary fiber in kelp in preparing medicine and functional food for improving ulcerative colitis. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a group of gastrointestinal diseases characterized by chronic inflammation and intestinal flora dysbiosis, including Crohn's disease (Crohn's disease, CD) and ulcerative colitis (Ulcerative colitis) , UC). Studies have shown that genetic, environmental, microbial factors, immune response, and dietary factors are involved in the pathogenesis of IBD. Epidemiological studies have shown that unreasonable diet structure and lack of dietary components are the main risk factors leading to IBD. At present, the incidence of IBD is significantly increasing worldwide. Inflammatory drugs, immunosuppressants, and biological drugs can all be used in the treatment of...

Claims

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Application Information

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IPC IPC(8): A61K36/03A61P1/04A61P1/00A61P29/00A23L33/21
CPCA61K36/03A61P1/04A61P1/00A61P29/00A23L33/21A23V2002/00A23V2200/3202A23V2250/5116
Inventor 缪锦来曲长凤曹峻菡何英英郑洲李江张丽萍王凯王西西秦玲
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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