High-density and high-yield porphyridium culture method

Abstract

The invention discloses a high-density and high-yield porphyridium continuous culture method. The method comprises the following steps: step 1, culturing porphyridium in a photobioreactor at high density to the middle and later periods of a logarithmic phase; step 2, continuously harvesting algae cell parts in high-density algae liquid; step 3, harvesting soluble exopolysaccharides in a supernate obtained by continuous harvesting, and supplementing the harvested supernate back to the photobioreactor; step 4, controlling the speed of feeding a culture medium concentrated solution into the photobioreactor; and repeating the steps 2-4 until the culture is ended. The method has the characteristics of high density, high yield and continuous production, can simultaneously harvest purple ball biomass, phycoerythrin, exopolysaccharide, polyunsaturated fatty acid and the like at high yield, realizes reuse of the culture solution, and improves the economical efficiency and environmental protection property of culture.

Description

technical field [0001] The present application relates to the field of microalgae biology, in particular to a high-density and high-yield method for cultivating Porphyridium algae. Background technique [0002] Porphyridium is a kind of unicellular microalgae that shows purple or deep red in different growth stages, and belongs to Rhodophyta, Protoerythrophyta, Porphyridium, Porphyridaceae, and Porphyridium genus. It has strong environmental adaptability and rapid reproduction ability, and is widely distributed in waters and moist soils all over the world. Significantly affected. The algae cells are often aggregated together irregularly, covered by a thin film, which is crust-like when dry. Porphyridum asexually reproduces by cell division. The cells are usually spherical, with a diameter of 5-24 μm, blood-red or dark purple-red, with an axonal star-shaped or irregular-shaped pigment body and an unsheathed protein nucleus. . The optimum temperature for the growth of Porp...

AI Technical Summary

Problems solved by technology

Therefore, on the whole, the yield of the product cannot be significantly improved.

[0009] (2) Unable to take into account the target product

[0012] (3) The culture medium cannot be recycled, resulting in large waste and pollution

[0013] In the traditional one-step (batch) culture process, the medium is difficult to recycle, resulting in waste of raw materials

Untreated discharge will cause pollution of the receiving water body, and water treatment of the culture medium will greatly increase the production cost and restrict the development of industrialization

[0014] (4) High unit energy consumption and high cost

[0015] Regardless of batch culture or semi-continuous culture, high-speed centrifugation is generally used to harvest algae cells. Due to the low culture density and large water volume, the energy consumption is extremely high, which greatly pushes up the cost.

Especially when harvesting in batches, the production load is heavy, the high-speed centrifugal equipment needs to run at high load for a long time, the personnel need to work overtime for a long time, and there is a situation that the algae liquid cannot be harvested in time and wasted

These all lead to lower cultivation efficiency and higher cost

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