High-density and high-yield porphyridium culture method

A technology of high-density culture and culture method, which is applied in the field of high-density and high-yield Porphyridium algae culture, can solve the problems of inability to recycle the culture medium, restrict the development of industrialization, and difficult to recycle the medium, and achieve high cell density and Product yield, addressing nutrient limitation issues, mitigation of inhibitory and toxic effects

Pending Publication Date: 2021-12-10
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, on the whole, the yield of the product cannot be significantly improved.
[0009] (2) Unable to take into account the target product
[0012] (3) The culture medium cannot be recycled, resulting in large waste and pollution
[0013] In the traditional one-step (batch) culture process, the medium is difficult to recycle, resulting in waste of raw materials
Untreated discharge will cause pollution of the receiving water body, and water treatment of the culture medium will greatly increase the production cost and restrict the development of industrialization
[0014] (4) High unit energy consumption and hig...

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  • High-density and high-yield porphyridium culture method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment one (improve inoculation density)

[0091] The comparative experiment was carried out in the same 1L glass bubbling column reactor as the comparative example. Light irradiation on both sides, the light intensity on the surface of the reactor is 400μE m -2 the s -1 . Aeration was carried out by bubbling through a stainless steel tube with a diameter of 2 mm extending into the bottom of the reactor.

[0092] The marine microalga Porphyridium cruentum is cultivated in the reactor, and the culture medium is ASW medium with potassium nitrate as the nitrogen source. The filling volume is 700mL, and the inoculation density is increased to 1.4g L -1 . CO 2 The volume fraction is 5% air carbon dioxide mixture, the ventilation volume is 0.14L min -1 , control the culture temperature at 25±1°C. The content of potassium nitrate and potassium dihydrogen phosphate is monitored during the process. When the content drops to 1 / 2 of the initial value, the above-mention...

Embodiment 2

[0093] Embodiment two (improve inoculation density 2)

[0094] The comparative experiment was carried out in the same 1L glass bubbling column reactor as the comparative example. Light irradiation on both sides, the light intensity on the surface of the reactor is 400μE m -2 the s -1 . Aeration was carried out by bubbling through a stainless steel tube with a diameter of 2 mm extending into the bottom of the reactor.

[0095] The marine microalga Porphyridium cruentum is cultivated in the reactor, and the culture medium is ASW medium with potassium nitrate as the nitrogen source. The filling volume is 700mL, and the inoculation density is 2g L -1 . CO 2 The volume fraction is 5% air carbon dioxide mixture, the ventilation volume is 0.14L min -1 , control the culture temperature at 25±1°C. The content of potassium nitrate and potassium dihydrogen phosphate is monitored during the process. When the content drops to 1 / 2 of the initial value, the above-mentioned nutrient c...

Embodiment 3

[0096] Embodiment three (improve inoculation density)

[0097] The comparative experiment was carried out in the same 1L glass bubbling column reactor as the comparative example. Light irradiation on both sides, the light intensity on the surface of the reactor is 400μE m -2 the s -1 . Aeration was carried out by bubbling through a stainless steel tube with a diameter of 2 mm extending into the bottom of the reactor.

[0098] The marine microalga Porphyridium cruentum is cultivated in the reactor, and the culture medium is ASW medium with potassium nitrate as the nitrogen source. The filling volume is 700mL, and the inoculation density is 2.6g L -1 . CO 2 The volume fraction is 5% air carbon dioxide mixture, the ventilation volume is 0.14L min -1 , control the culture temperature at 25±1°C. The content of potassium nitrate and potassium dihydrogen phosphate is monitored during the process. When the content drops to 1 / 2 of the initial value, the above-mentioned nutrient...

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Abstract

The invention discloses a high-density and high-yield porphyridium continuous culture method. The method comprises the following steps: step 1, culturing porphyridium in a photobioreactor at high density to the middle and later periods of a logarithmic phase; step 2, continuously harvesting algae cell parts in high-density algae liquid; step 3, harvesting soluble exopolysaccharides in a supernate obtained by continuous harvesting, and supplementing the harvested supernate back to the photobioreactor; step 4, controlling the speed of feeding a culture medium concentrated solution into the photobioreactor; and repeating the steps 2-4 until the culture is ended. The method has the characteristics of high density, high yield and continuous production, can simultaneously harvest purple ball biomass, phycoerythrin, exopolysaccharide, polyunsaturated fatty acid and the like at high yield, realizes reuse of the culture solution, and improves the economical efficiency and environmental protection property of culture.

Description

technical field [0001] The present application relates to the field of microalgae biology, in particular to a high-density and high-yield method for cultivating Porphyridium algae. Background technique [0002] Porphyridium is a kind of unicellular microalgae that shows purple or deep red in different growth stages, and belongs to Rhodophyta, Protoerythrophyta, Porphyridium, Porphyridaceae, and Porphyridium genus. It has strong environmental adaptability and rapid reproduction ability, and is widely distributed in waters and moist soils all over the world. Significantly affected. The algae cells are often aggregated together irregularly, covered by a thin film, which is crust-like when dry. Porphyridum asexually reproduces by cell division. The cells are usually spherical, with a diameter of 5-24 μm, blood-red or dark purple-red, with an axonal star-shaped or irregular-shaped pigment body and an unsheathed protein nucleus. . The optimum temperature for the growth of Porp...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P19/04C12P7/64C12P21/02C12R1/89
CPCC12N1/12C12P19/04C12P7/6427C12P21/02
Inventor 章真张荣庆张静刘晓军陈夏姚丽萍
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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