Fluorescent probe for use in detection of brain tumor

Abstract

[Problem] To provide a novel fluorescent probe which can be used in a spray mode, has excellent specificity to sensitivity, also has immediacy, and enables the detection of brain tumor. [Solution] A fluorescent probe for use in the detection of brain tumor, which comprises a compound represented by formula (I) or a salt thereof: (in the formula, P1 represents an arginine residue, a histidine residue or a tyrosine residue, and P2 represents a proline residue or a glycine residue, wherein P1 is liked to an adjacent N atom to form an amide bond, and P2 is linked to P1 to form an amide bond; R1 represents 1 to 4 same or different substituents independently selected from the group consisting of a hydrogen atom and an alkyl group, a carboxyl group, an ester group, an alkoxy group, an amide group and an azide group each of which may be substituted; R2, R3, R4, R5, R6 and R7 independently represent a hydrogen atom, a hydroxyl group, an alkyl group which may be substituted, or a halogen atom; R8 and R9 independently represent a hydrogen atom or an alkyl group; X represents O, Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb) or P(=O)(Ra); Ra and Rb independently represent a hydrogen atom, an alkyl group or an aryl group; and Y represents a C1-C3 alkylene group).

Description

technical field [0001] The present invention relates to fluorescent probes for the detection of brain tumors. In more detail, it relates to a fluorescent probe capable of specifically detecting and labeling brain tumors by coating or spraying a tissue sample, and a detection method using the fluorescent probe. Background technique [0002] Gliomas (gliomas) account for about 30% of primary brain tumors, and the median survival of the most malignant glioblastomas is about 1.5 years. Even low-grade gliomas are rarely completely cured, and most deteriorate within a few years and die within 5-10 years. In glioma surgery, although total resection is a favorable prognostic factor, extensive resection of the surrounding brain cannot be performed due to functional limitations, and visualization of the tumor has been attempted to achieve safer and maximized resection. [0003] To date, 5-aminolevulinic acid (5-ALA) is the only insurance coverage for use as a fluorescent reagent for...

AI Technical Summary

Problems solved by technology

However, the method using 5-ALA has the problem of sensitivity and specificity, the necessity of oral administration before surgery, the duration of metabolism, and the difficulty of re-administration. In addition, in the case of low-risk tumors, there is a problem that fluorescence cannot be performed. Problems of labeling etc. (for example, Non-Patent Documents 1 and 2)

Moreover, traditional tumor visualization methods are often difficult to balance the improvement of resection rate and the preservation of brain nerve function in a trade-off relationship

Images