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Microbial method capable of efficiently decolorizing gentian violet in dye wastewater

A technology for dye wastewater and gentian violet, applied in the field of microbial engineering, can solve the problems of low safety and high cost, and achieve the effects of reducing pollution, no secondary pollution, and avoiding environmental pollution

Pending Publication Date: 2022-02-11
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention provides a microbial method capable of efficiently decolorizing gentian violet in dye wastewater to solve the problems of low safety and high cost in the current treatment of printing and dyeing wastewater

Method used

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  • Microbial method capable of efficiently decolorizing gentian violet in dye wastewater
  • Microbial method capable of efficiently decolorizing gentian violet in dye wastewater
  • Microbial method capable of efficiently decolorizing gentian violet in dye wastewater

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preparation example Construction

[0018] The preparation of LB high-salt liquid culture medium of the present invention is: yeast powder 10g, peptone 5g, sodium chloride 5g, pH7.0, water is settled to 1L, 121 ℃ of sterilization 30min, cooling;

[0019] The preparation of gentian violet dye aqueous solution of the present invention is: 1ml of gentian violet dye storage solution is fixed to 1L with double-distilled water, and sterilized by 450nm membrane filtration;

[0020] The preparation of gentian violet dye storage liquid of the present invention is: 10 g of gentian violet, 100 ml of double distilled water, natural pH, fully dissolved, 450nm membrane filter sterilization, storage for later use;

[0021] The preparation of the strain culture solution of the present invention is as follows: inoculate Comamonas testosteroni (Comamonastestosteroni) CT1 strain in LB high-salt liquid medium, and shake the flask at 37° C. for 24 hours;

[0022] The pH of the shake flask culture of the present invention is 7.0.

...

experiment example 1

[0024] Activation of experimental example 1 bacterial strain and biological decolorization of gentian violet dye

[0025] 1. Strain activation

[0026] Comamonas testosteroni CT1 and KF-1, Vibrio H5, Buttiauxella S19-1, Pseudomonas sp LY1, Acinetobacter calcoacetate ( Acinetobacter calcoaceticus) LM1, Rhodococcus sp. P14, Pseudomonas sp. JP1, the above 8 strains were screened from the natural environment and proved to be harmless to humans;

[0027] The above-mentioned strains were taken out from the -80°C refrigerator, thawed at room temperature, added to LB medium, and cultured overnight at 37°C in a 180r / min shake flask to complete the activation of the bacteria. Carry out subculture the next day (method is the same as above);

[0028] The composition of described LB high-salt liquid culture medium is: yeast powder 10g, peptone 5g, sodium chloride 5g, pH 7.0, water is settled to 1L. Sterilize at 121°C for 30 minutes and cool down.

[0029] 2. Degradation experiment of s...

experiment example 2

[0032] Experimental example 2 Comamonas testosteroni (Comamonas testosteroni) CT1 decolorization effect experiment on gentian violet at different temperatures

[0033] 1. Inoculate the bacterial strain of Comamonas testosteroni (Comamonas testosteroni) CT1 in a liquid medium, and culture it on a shaker at 37° C. and 180 rpm for 24 hours to obtain the culture medium of the strain; the composition of the liquid medium is: Yeast powder 10g, peptone 5g, sodium chloride 10g, pH 7.0, water to 1L;

[0034] 2. Take CT1 culture medium and adjust OD 600 =1.0, according to the inoculum amount of 1:100, insert it into the aqueous solution containing 100mg / L gentian violet, respectively place it at different temperatures (22°C, 27°C, 32°C, 37°C), and cultivate it at 180rpm (three parallel experiments) , measure the absorbance value every 1d;

[0035] 3. The decolorization ability of CT1 is calculated by the decolorization rate. Above-mentioned solution was centrifuged at 3000rpm 10 minu...

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Abstract

The invention relates to a microbial method capable of efficiently decolorizing gentian violet in dye wastewater, and belongs to the technical field of microbial engineering. The method comprises the following steps: carrying out subculture on a comamonas testosteroni CT1 activated strain by using an LB high-salt liquid culture medium, adjusting the OD600 of the strain to be 1.0, taking a strain culture solution, inoculating the strain culture solution into a gentian violet dye aqueous solution according to the inoculum size of 1: 100, and carrying out shake-flask culture at 37 DEG C for 72 hours, so that the degradation rate of gentian violet is 74%. The method has the beneficial effects that a natural strain, namely comamonas testosteroni CT1 with gentian violet decoloring capability is selected through screening by simulating a wastewater high-salt environment, gentian violet in triphenylmethane dyes can be quickly decolored, the decoloring capability of the strain and the decoloring efficiency of gentian violet are improved by changing the optimal decoloring condition of the strain, the environmental pollution caused by the dye and the risk brought to the human health are effectively reduced, the environmental pollution caused by the dye molecules directly entering the water body can be effectively avoided, the secondary pollution is avoided, and the ecological efficiency and the application prospect are good.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, in particular to a biological decolorization technology for gentian violet in dye wastewater. Background technique [0002] With the rapid development of the printing and dyeing industry, the total output of dyes in my country has reached 990,000 tons in 2017, and the printing and dyeing industrial wastewater brought about by the production of dyes is about 740 million cubic meters. Triphenylmethane dyes are the third dye type after azo dyes and anthraquinone dyes. Commonly used are gentian violet, malachite green, bromophenol blue, Victoria blue and so on. These dyes are often carcinogenic, not only harmful to human health, but also destroy the ecological environment to a large extent, and cause great damage to soil and water bodies. Gentian violet is also known as hexamethyl violet, methyl violet 10B, and its molecular formula is C 25 h 30 N 3 Cl 9H 2 O, is currently the mos...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/34C02F2101/308
Inventor 郭立泉王佳琦赵超越刘东马传福闻增昕任苡宣
Owner JILIN AGRICULTURAL UNIV
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