Preparation method and application of beer-based nitrogen-phosphorus co-doped biomass carbon dots
A biomass carbon, co-doping technology, applied in chemical instruments and methods, nanocarbon, fluorescence/phosphorescence, etc., can solve the problems of increased operation and cost, detection performance disadvantage, etc., to achieve low cost, rich surface functional groups, fluorescence The effect of high intensity and fluorescence quantum yield
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Example Embodiment
[0046] Example one
[0047] This embodiment provides a method for preparing a beer base nitrogen phosphorus-doped biocontrol carbon dot, specifically:
[0048] Take more than 100 ml of the commercially available beer, filter beer with 0.22 microns of microporous membrane to get a beer filtrate;
[0049] 50 ml of the beer filtrate was added to the Teflon liner stainless steel high pressure reactor, and after sealed the reactor, it was placed in a blower drying tank at 160 ° C for 6 hours, resulting in a pale yellow solution, naturally cooled to room temperature. ;
[0050] The pale yellow solution was 0.22 micrometers of microporous membrane and centrifuged; the centrifugal supernatant was dialyzed in pure water for 12 hours, and the molecular interception was 500 Da to obtain dialysate;
[0051] The obtained dialysate was carried out at -80 ° C lyophilized and lyophilized to obtain a powder-shaped beer base nitrogen phosphorus.
[0052] See figure 1 , For the preparation of the pr...
Example Embodiment
[0060] Example 2
[0061] This example discloses the use of beer base nitrogen phosphorus test detection Fe 3+ The method is specifically: to the centric tube sequentially add 1 ml of the beer base nitrogen-doped biopsy, 300 microliter phosphate buffer solution (10 mmol, pH) 7.4), mix uniform;
[0062] Prepare 0 to 400 micromolar per liter of Fe 3+ Several parts of the standard solution, the mixed buffer solution is added to different concentrations of Fe 3+ In the standard solution, the shock is shaken, allowed to stand for 5 minutes;
[0063] The fluorescence spectrum of the solution was determined for each standard solution, and the excitation wavelength was 370 nm.
[0064] image 3 For different concentrations Fe 3+ Quenching effect of standard solution on the fluorescence intensity of beer base nitrogen phosphorus. The linear regression equation is Y = -0.0013x + 0.4431, linear correlation coefficient r 2 = 0.9931, for Fe 3+ The detection limit is 0.14 micromolar, which can ...
Example Embodiment
[0065] Example three
[0066] This example discloses a method of detecting ascorbic acid with beer base nitrogen phosphorus carbon dot, specifically:
[0067] The beer base nitrogen phosphors prepared in Example 2 were sequentially added to the centrifugal tube 1 ml, 300 μl phosphate buffer solution (a concentration of 10 mmol, pH of 7.4), mixed uniform;
[0068] Prepare 0 to 500 micromolar per liter of Fe 3+ Several of the standard solution, as a fluorescent quencher, the mixed buffer solution is added to different concentrations of Fe 3+ In the standard solution, the shock is shaken, allowed to stand for 5 minutes;
[0069] A total of 0 to 300 micromollers per liter of ascorbic acid standard solution, as a fluorescent recovery agent, after the azitrose having a copper-doped biocarbal carbon dot, the fluorescence quenched solution was added to different The concentration of ascorbic acid standard solution is went, shakes well, incubated at room temperature for 5 minutes to 10 min...
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