PH-responsive nanoparticle self-assembled aggregate molecular probe, and preparation method and application thereof

A nanoparticle and molecular probe technology, which is applied in the field of nanoparticle self-assembled aggregate molecular probes and its preparation, can solve the problems of limiting the application range and prospects of nanomaterials, and achieve the effect of avoiding precipitation

Pending Publication Date: 2022-02-25
WUXI PEOPLES HOSPITAL
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  • Abstract
  • Description
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Problems solved by technology

At the same time, there have been a lot of reports on the nuclide labeling of gold nanoparticles. Most of the research is

Method used

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  • PH-responsive nanoparticle self-assembled aggregate molecular probe, and preparation method and application thereof
  • PH-responsive nanoparticle self-assembled aggregate molecular probe, and preparation method and application thereof
  • PH-responsive nanoparticle self-assembled aggregate molecular probe, and preparation method and application thereof

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preparation example Construction

[0036] The invention provides a preparation method of a pH-responsive nanoparticle self-assembled aggregate molecular probe, comprising the following steps:

[0037] The nanoparticle stock solution, methoxy polyethylene glycol mercaptan and amino polyethylene glycol mercaptan are mixed and modified to obtain a mother solution containing amino functionalized nanoparticles on the surface;

[0038] Reacting the mother liquor containing amino-functionalized nanoparticles on the surface, the substance containing pH-responsive groups and triethylamine to obtain nanoparticles modified with pH-responsive groups; the substances containing pH-responsive groups include acid anhydrides;

[0039] Mixing the pH-responsive group-modified nanoparticle, water and pH buffer solution for self-assembly to obtain the pH-responsive nanoparticle self-assembled aggregate molecular probe.

[0040] In the invention, the nanoparticle stock solution, methoxy polyethylene glycol mercaptan and amino polyet...

Embodiment 1

[0075] Example 1: Preparation of gold nanoparticles and PEG modification of their surfaces.

[0076] Add 1wt% chloroauric acid solution (0.6mL) to ultrapure water (100mL), heat to 100°C, stir vigorously to boiling, add 1wt% sodium citrate solution (3mL), and wait until the system turns wine red , continue to boil for 30min to obtain gold nanoparticle stock solution.

[0077] After cooling, M-PEG was added to the gold nanoparticle stock solution (100 mL, containing 1 mg of gold nanoparticles) in turn. 5000 -SH (20mg) and NH 2 -PEG 5000 -SH (20 mg), stirred at room temperature for 24 h. The excess PEG was removed by ultrafiltration centrifugation (5000rpm×10min) 3 times. After centrifugation, it was resuspended in ultrapure water to obtain the PEG-modified amino-functionalized gold nanoparticle mother solution.

Embodiment 2

[0078] Example 2: PEG terminal modification of pH-responsive groups on the surface of gold nanoparticles.

[0079] To the PEG-modified amino-functionalized gold nanoparticle mother solution (100 mL, containing 2.3 mg of PEG-modified amino-functionalized gold nanoparticles) prepared in Example 1, the pH-responsive group dimethylmaleamide (dimethylmaleamide) was added. 0.36 mg) and triethylamine (5.0 μL), and the reaction was stirred at room temperature for 2 h. After ultrafiltration centrifugation (5000rpm×10min) for 3 times, gold nanoparticles modified with pH-responsive groups were obtained. By measuring the concentration of pH-responsive groups in the solution before and after the reaction, it was calculated that 0.75 μmol of pH-responsive groups were coupled to the surface of the nanoparticles.

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Abstract

The invention provides a pH-responsive nanoparticle self-assembled aggregate molecular probe, and a preparation method and application thereof, and belongs to the technical field of nanoparticle self-assembly. The surfaces of nanoparticles are modified with a substance containing a pH response group, self-assembly of light-triggered nanoparticles is achieved, and the obtained pH response nanoparticle self-assembled aggregate absorbs wavelength red shift, has a photo-thermal treatment effect, and is successfully applied to photoacoustic imaging and photo-thermal treatment research of living tumors, meanwhile, the nano-molecular probe is successfully prepared by utilizing radionuclide labeling, and the nano-molecular probe has important guiding significance on metal nano-materials in clinical tumor diagnosis and treatment research.

Description

technical field [0001] The invention relates to the technical field of nanoparticle self-assembly, in particular to a pH-responsive nanoparticle self-assembled aggregate molecular probe and a preparation method and application thereof. Background technique [0002] The ever-increasing morbidity and high mortality of tumors make early diagnosis and treatment of tumors particularly important. With the rapid development of nanotechnology, nanomaterials show broad application prospects in tumor diagnosis and treatment due to their unique physical and chemical properties. For example, gold nanomaterials have unique physical and chemical properties such as light, electricity, heat, and catalysis, and have good biocompatibility. They are important components for building new composite functional materials. Photothermal therapy, targeted drug loading, photochemical catalysis and other fields have shown broad application prospects. Due to the good biocompatibility of gold nanoparti...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/60A61K49/22A61K51/06A61P35/00
CPCA61K49/225A61K51/065A61K41/0052A61K47/60A61P35/00
Inventor 陈飞朱宝浦晓佳邵科晶
Owner WUXI PEOPLES HOSPITAL
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