A kind of heparinized PVC material, its preparation method and its application as medical equipment
A PVC material and heparinization technology, applied in the field of medical devices, can solve the problems of residual covalent bond, heparin shedding, lack of adjustment of the amount of heparin grafting, etc., achieving low cost, good anticoagulation effect, and reducing treatment costs Effect
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Embodiment 1
[0054] Using plasma treatment equipment, ordinary medical PVC materials were modified for 30min under the conditions of voltage amplitude of 4KV, voltage frequency of 40KHz, duty cycle of 40%, modulation frequency of 100Hz and pure helium environment to obtain activated PVC materials with hydroxyl groups on the surface.
[0055] Dissolve 1g heparin sodium in 100ml deionized water to form an aqueous solution of heparin sodium with a mass fraction of 1%, and then adjust the pH of the solution to 1.0 with sulfuric acid. The hydroxyl modified PVC material obtained by plasma treatment in the above steps is immersed in acidic heparin sodium solution, and concentrated sulfuric acid is added as catalyst at the temperature of 60 ℃. After stirring for 6 hours, the base hydroxyl modified heparin anticoagulant catheter ② is obtained, and the unmodified PVC material is ①.
Embodiment 2
[0057] Under the conditions of voltage amplitude of 4KV, voltage frequency of 40KHz, duty cycle of 40%, modulation frequency of 100Hz and mixed environment of helium and 3% oxygen, ordinary medical PVC materials were modified for 30min by plasma treatment equipment to obtain activated PVC materials with carboxyl groups on the surface.
[0058] Dissolve 1g heparin sodium in 100ml deionized water to form an aqueous solution of heparin sodium with a mass fraction of 1%, and then adjust the pH of the solution to 1.0 with sulfuric acid. The carboxyl modified PVC material obtained by plasma treatment in the above steps is immersed in acidic heparin sodium solution, and concentrated sulfuric acid is added as catalyst at the temperature of 60 ℃. After stirring for 6 hours, the base carboxyl modified heparin anticoagulant catheter ③ is obtained, and the unmodified PVC material is ①.
Embodiment 3
[0060] Plasma modification is the same as example 2.
[0061] Dissolve 1g of sodium alginate with viscosity coefficient of 1% and viscosity of 3 ~ 5MPa · s in 100ml deionized water to form a sodium alginate solution with mass fraction of 1%, adjust the pH of the sodium alginate solution to 2 with sulfuric acid, soak the PVC material modified by carboxyl group in the sodium alginate solution in the above steps, add concentrated sulfuric acid as catalyst at 60 ℃ and stir for 6 hours to obtain the PVC material grafted by sodium alginate, It was washed with deionized water and dried in vacuum at room temperature. Put the dried sodium alginate grafted PVC material into the sodium heparin solution with pH of 1.0, add concentrated sulfuric acid as catalyst at the temperature of 60 ℃, stir the reaction for 4h, wash it with deionized water, and then soak it in calcium chloride solution with mass fraction of 2% for crosslinking for 1H. Finally, clean the crosslinked modified catheter with d...
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