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Actinomycete strain SCAUT001 and application thereof

A technology of actinomycetes and strains, applied in the field of microorganisms, to achieve good application potential, promote nodulation and growth

Active Publication Date: 2022-03-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since A. glabra is a leguminous forage, previous studies mainly focused on the symbiotic relationship between rhizobia and A. glabra, but there is no report on the effect of rhizosphere growth-promoting bacteria on the growth of A. glabra.

Method used

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  • Actinomycete strain SCAUT001 and application thereof
  • Actinomycete strain SCAUT001 and application thereof
  • Actinomycete strain SCAUT001 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The separation and purification of embodiment 1 rhizosphere actinomycete SCAUT001

[0030] 1.1 Isolation of actinomycetes from the rhizosphere of Vitex glabrata in Liangshan

[0031] In 2018, the purple vetch was collected in Huidong County, Liangshan Yi Autonomous Prefecture, Sichuan Province. The excess soil on the root system was shaken off, and the root was placed in a 50mL sterilized centrifuge tube filled with 25mL phosphate buffer solution, and treated with ultrasonic waves (150W) After 10 minutes, the roots were taken out under sterile conditions, and the supernatant was removed by centrifugation at 3000g for 10 minutes. The sediment was rhizosphere soil, and made 10 -1 、10 -2 , 10 -3 For gradient dilution, 100 ul of the suspension was uniformly spread on the modified Gowell's medium for separation, and each dilution concentration was repeated three times, and cultured in a constant temperature incubator at 28°C for 5-7 days. Pick the strains with typical act...

Embodiment 2

[0048] The growth-promoting function screening of embodiment 2 rhizosphere actinomycete SCAUT001

[0049] 2.1 Screening of growth-promoting function

[0050] 2.1.1 Determination of IAA Production Ability

[0051] Take 20 μL of bacterial suspension and insert it into ISP containing 0.5mol / L tryptophan 4 In liquid culture medium, repeated 3 times, set blank control, cultured on a shaker at 28°C at 120r / min for 3d, centrifuged at 8000r / min for 24h, took 1mL of bacterial supernatant, added 2mL of IAA chromogenic solution, reacted in dark at 25°C for 30min, 530nm Colorimetric at the wavelength and record the absorbance value. With the uninoculated liquid culture medium as the control zero adjustment, the indole acetic acid standard solution with the concentration of 0, 5, 20, 40, 60mg / L is the same as above to make the calibration curve, and calculate the concentration of IAA in the measurement solution.

[0052] 2.1.2 Determination of siderophore production capacity

[0053] D...

Embodiment 3

[0084] Embodiment 3 hydroponic experiment of purple sweet potato

[0085] 3.1 Hydroponics experiment

[0086] The actinomycete strains to be tested were first connected to the ISP 4 Activated on plate medium, then transferred to ISP 4 Cultivate overnight in liquid medium to the logarithmic phase, the growth of the strains is detected by the plate method, and the bacterial content is calculated.

[0087] After soaking the seeds of Viola glabrata overnight, carry out surface disinfection, use sterile tweezers to grasp the seeds and plant them in a petri dish equipped with filter paper and sterile vermiculite, plant 10 seeds in each petri dish, and then add sterile water to keep moisture , and maintain air circulation. Sterilize the tweezers, filter paper, petri dishes, plastic cups, vermiculite, quartz sand, and low-nitrogen nutrient solution required for the experiment.

[0088] Low nitrogen nutrient solution formula: KNO 3 10.1g, KH 2 PO 4 2.2g, MnSO 4 ·H 2 O 100.0m...

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Abstract

The invention discloses an actinomycete strain Williamsia herbipolysis SCAUT001, which is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 61375. The actinomycete strain Williamsia herbipolysis SCAUT001 is named as Williamsia herbipolysis SCAUT001. The Williamsia herbipolysis SCAUT001 provided by the invention through separation and screening has growth promoting capacities of generating IAA and siderophores, dissolving phosphorus, degrading cellulose and chitin and the like, and also has stress resistance functions of salt resistance, acid and alkali resistance, drought resistance and the like, and by applying the Williamsia herbipolysis SCAUT001 to the Liangshan vicia villosa, the effect of promoting nodulation and growth of the Liangshan vicia villosa can be achieved, and the Williamsia herbipolysis SCAUT001 can be applied to the Liangshan vicia villosa. The yield of forage grass is increased, meanwhile, the soil quality is improved, the tobacco planting soil is improved, and the method has good application potential in agricultural production.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an actinomycete strain SCAUT001 and an application thereof. Background technique [0002] Liangshan Prefecture is one of the three major pastoral areas in Sichuan Province. Animal husbandry has a long history and is one of the main sources of cash income for farmers and herdsmen. Especially in high mountain areas, cash income from animal husbandry accounts for more than 70% of rural household income. Because Liangshan Prefecture has the climate characteristics of dry winter and spring, rainy summer, and distinct dryness and wetness, the problem of lack of green fodder for livestock during winter and spring is very prominent. However, in the mountainous areas around the basin of Liangshan Prefecture, especially in the Zhongshan and sub-alpine areas at an altitude of 1700-3200m, a large amount of land is left idle during winter and spring. Viciavillosa var. glabresens is ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G22/40C12R1/01
CPCC12N1/20A01G22/40
Inventor 赵珂冯文龙辜运富杨军伟陈强闫芳芳张宗锦张紫薇余秀梅向泉桔张凌子
Owner SICHUAN AGRI UNIV
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