Efficient decomposition liquid bacterial fertilizer as well as preparation method and application thereof

A liquid bacteria, high-efficiency technology, applied in liquid fertilizers, biochemical equipment and methods, applications, etc., to achieve high market promotion and application value, stable function, and improve the effect of degradability

Active Publication Date: 2022-03-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention mainly aims at the problems of lack of nitrogen source and lignocellulose intermediate product metabolizing bacteria in the above-mentioned straw inoculum components, and provides a preparation method for highly decomposing liquid bacterial manure , on the one hand, the bacterial fertilizer solution already contains the quick-acting and slow-release nitrogen sources required for microbial growth and metabolism, and can effectively improve the decomposing efficiency of straw without additional nitrogen sources during application; on the other hand, a variety of Metabolic pathways and the complementarity of different degradation enzyme systems, Pediococcus pentosaceae can effectively degrade the intermediate metabolites of straw lignocellulose, such as low molecular weight oligosaccharides, lactic acid, ethanol, etc., and relieve the repression effect caused by the accumulation of metabolites

Method used

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  • Efficient decomposition liquid bacterial fertilizer as well as preparation method and application thereof
  • Efficient decomposition liquid bacterial fertilizer as well as preparation method and application thereof
  • Efficient decomposition liquid bacterial fertilizer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation and Function Verification of Corn Stalk Bacterial Fertilizer

[0040] Stenotrophomonas, Achromobacter and lactic acid bacteria independently screened by the laboratory were combined with key functional strains in the straw decomposition process, namely Bacillus subtilis (biocontrol bacteria), Streptomyces roseus flavum (biocontrol bacteria), Trichoderma harzianum (biocontrol fungus), Penicillium picoides (cold-resistant type), and Myceliophthora thermophila (thermophilic type) were combined in pairs to carry out the plate confrontation test. The results showed that there was no antagonistic effect between the strains, and the Prerequisites for assembly.

[0041] The above 8 strains are subjected to strain activation, seed culture, expanded culture and mixed fermentation to prepare liquid bacterial fertilizer.

[0042] According to aseptic operation, use sterile water to expand the cultivation of the bacterial fertilizer with Stenotrophomonas, Achromobacter,...

Embodiment 2

[0051] The liquid bacterial fertilizer prepared by the present invention was compared with three kinds of commercially available organic material decomposing agents for liquid fermentation degradation comparison test. In the test, the amount of substrate, the amount of bacterial agent inoculated, and the culture conditions were all consistent, and 3 replicates were set. .

[0052] Nutrient solution: 0.5% peptone, 0.5% NaCl, 0.2% CaC0 3 , 0.1% yeast powder, natural pH

[0053] Test method: Weigh 1g of the substrate and add it to a conical flask filled with 45mL of nutrient solution, and sterilize at 121°C for 20 minutes. The bacterial fertilizer prepared by the present invention and the commercially available organic material decomposing agent are diluted with sterile water to adjust to the same concentration Inoculate 5mL of the suspension in each Erlenmeyer flask, take a sample after incubating on a shaker at 30°C and 150 r / min for 10 days, add an appropriate amount of sulfu...

Embodiment 3

[0058] The liquid bacterial fertilizer is applied to the rapid and efficient decomposition of rice, wheat, and corn stalks. The preferred field application temperature range is 5-25° C., and no additional nitrogen source is needed when the liquid bacterial fertilizer is applied. The application method of liquid bacterial manure is spraying on the surface of straw and doing compost fermentation. The fermentation method can use conventional fermentation means and devices, and the whole operation is simple, convenient to use, and easy to popularize and use.

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Abstract

The invention discloses a high-efficiency decomposition liquid bacterial fertilizer and a preparation method thereof, the bacterial fertilizer comprises bacillus subtilis, streptomyces roseoflavus, trichoderma harzianum, eupenicillium capitatum, thermophilic mycelia and pediococcus pentosaceus, and the effective viable count is greater than or equal to 1 * 10 < 9 > cfu / mL. The method has the advantages that the prepared bacterial fertilizer liquid contains sufficient nitrogen sources required by growth and metabolism of microorganisms, and the decomposition efficiency of straw can be effectively improved without additionally supplementing the nitrogen sources during application; on the other hand, various metabolic pathways of different strains and complementation on different degrading enzyme systems are fused, the pediococcus pentosaceus can effectively degrade intermediate metabolites of straw lignocellulose, such as low-molecular oligosaccharide, lactic acid and ethyl alcohol, and the repression effect caused by product accumulation in the straw carbon metabolism process is relieved.

Description

technical field [0001] The invention belongs to the field of decomposing agents for organic materials or the field of liquid bacterial fertilizer, and in particular relates to a high-efficiency decomposing liquid bacterial fertilizer and its preparation method and application. Background technique [0002] Because the relevant national ministries and commissions have successively promulgated policies such as banning straw burning and comprehensive utilization of resources, the model of giving priority to returning straw to the field has developed rapidly. Straw is an agricultural by-product with a very high content of fiber components. The macromolecular chemical components cellulose, hemicellulose and lignin, which are difficult to decompose, have a compact and complex structure, high degree of polymerization, stable properties and strong resistance to decomposition. Under natural conditions, the decomposition rate of returning straw is slow, and a large amount of insuffici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/40C05G5/20C12N1/20C12N1/14C12R1/01C12R1/025C12R1/125C12R1/465C12R1/885C12R1/645
CPCC05G3/40C05G5/20C12N1/20C12N1/14C05C9/00C05D3/02C05D9/00C05F11/00C05F11/08C05F17/20Y02W30/40
Inventor 苏瑶杨艳华贾生强沈阿林
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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