Preparation method of degradable antibacterial hemostatic hydrogel for promoting diabetes wound repair

A wound repair and diabetes technology, applied in bandages, medical science, etc., can solve the problems of long healing time, high price, easy wound infection, etc., to avoid drug resistance, mild conditions, and accelerate wound healing.

Pending Publication Date: 2022-04-29
张 培华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are also wound healing hydrogels (Shukangbo, Ningbifu, Kanghuier, etc.) on the market, but due to the high price, there are still problems such as wound infection and long healing time.

Method used

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  • Preparation method of degradable antibacterial hemostatic hydrogel for promoting diabetes wound repair
  • Preparation method of degradable antibacterial hemostatic hydrogel for promoting diabetes wound repair
  • Preparation method of degradable antibacterial hemostatic hydrogel for promoting diabetes wound repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of oxidized hydroxyethyl cellulose (OHEC): Weigh (1.0g, 16mmol) hydroxyethyl cellulose (HEC) and stir to dissolve it in 300mL deionized water, add (1.71g, 8mmol) sodium periodate , reacted in the dark for 12 hours, then added an appropriate amount of 1.5mL ethylene glycol to terminate the reaction, the reaction product was dialyzed by a dialysis machine, and then OHEC was obtained by freeze-drying;

[0032] (2) DA grafted with OHEC: Add 1g OHEC and 0.05g DA into PBS, react at 37°C for 24h to obtain the target product (OHEC@DA);

[0033] (3) Dissolve JC in 0.1mol / L glacial acetic acid aqueous solution, use 0.1mol / L NaOH solution to adjust the pH to neutral, remove air bubbles by centrifugation, and obtain 2wt.% JC mixture; then take OHEC@DA and add deionized water To form a 2wt.% solution, take 5mL JC mixed solution and 5mL OHEC@DA solution respectively, add 1mL, 0.05% genipin, stir for 30min, transfer to a petri dish at room temperature for 24h to form a...

Embodiment 2

[0035] (1) Preparation of OHEC: Weigh (1.0g, 16mmol) HEC, stir and dissolve it in 300mL deionized water, add (1.71g, 8mmol) sodium periodate, and react in the dark for 12h, then add an appropriate amount of 1.5mL ethylene glycol to terminate Reaction, the reaction product is dialyzed through a dialysis machine, and then obtained OHEC by freeze-drying;

[0036] (2) DA grafted with OHEC: add 1g OHEC and 0.05g DA to PBS, and react at 37°C for 24h to obtain OHEC@DA;

[0037] (3) Dissolve JC in 0.1mol / L glacial acetic acid aqueous solution, use 0.1mol / L NaOH solution to adjust the pH to neutral, remove air bubbles by centrifugation, and obtain 2wt.% JC mixture; then take OHEC@DA and add deionized water To form a 2wt.% solution, take 10mL JC mixed solution and 5mL OHEC@DA solution respectively, add 1mL, 0.05% genipin and stir for 30min, then transfer to a petri dish at room temperature for 24h to form OHEC@DA / JC hydrogel.

Embodiment 3

[0039] (1) Preparation of OHEC: Weigh (1.0g, 16mmol) HEC, stir and dissolve it in 300mL deionized water, add (1.71g, 8mmol) sodium periodate, and react in the dark for 12h, then add an appropriate amount of 1.5mL ethylene glycol to terminate Reaction, the reaction product is dialyzed through a dialysis machine, and then obtained OHEC by freeze-drying;

[0040] (2) DA grafted with OHEC: add 1g OHEC and 0.05g DA to PBS, and react at 37°C for 24h to obtain OHEC@DA;

[0041](3) Dissolve JC in 0.1mol / L glacial acetic acid aqueous solution, use 0.1mol / L NaOH solution to adjust the pH to neutral, remove air bubbles by centrifugation, and obtain 2wt.% JC mixture; then take OHEC@DA and add deionized water To form a 2wt.% solution, take 15mL JC mixed solution and 5mL OHEC@DA solution respectively, add 1mL, 0.05% genipin and stir for 30min, then transfer to a petri dish at room temperature for 24h to form OHEC@DA / JC hydrogel.

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Abstract

The invention discloses a preparation method of degradable antibacterial hemostatic hydrogel capable of promoting diabetic wound repair. The hydrogel with a three-dimensional network structure is prepared from dopamine (DA) grafted oxidized natural polysaccharide and jellyfish collagen (JC) through a cross-linking agent. The hydrogel prepared by the invention not only has excellent tissue adhesion performance, antibacterial performance and hemostasis performance, but also has very strong water retention capacity, provides a moist environment for wound healing of diabetes mellitus, and accelerates wound healing. The invention has the advantages that: 1, the prepared degradable hydrogel with antibacterial and hemostatic functions for repairing the diabetic wound surface does not need to be additionally added with antibiotics when being used for treating the diabetic wound surface, so that the generation of drug resistance is avoided; 2, the used collagen is derived from plankton in an aquatic environment, and compared with animal-derived collagen, outbreak of zoonosis (mad cattle disease, porcine brucellosis, inhalable anthrax and the like) and some potential virus transmission risks are avoided;

Description

technical field [0001] The invention relates to the technical field of diabetic refractory wound repair, in particular to a preparation method of a degradable antibacterial hemostatic hydrogel for promoting diabetic wound repair. Background technique [0002] Difficult-to-heal wounds on diabetic feet due to microvascular damage and immune system imbalance in diabetic patients are the main reasons for amputation. Due to the lack of effective clinical treatment methods for wounds of diabetic patients, there is a high mortality rate; this not only causes serious psychological barriers and economic burdens to patients, but also brings a huge burden to the social medical system. Despite the continuous development of clinical wound healing materials, the existing wound healing materials cannot meet the expected requirements of modern medicine for perfect wound regeneration. Traditional wound dressing materials (bandages, cotton, and gauze) are very dry on the surface, exacerbatin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08J3/075C08L1/28C08B11/20
CPCC08J3/075C08B11/20A61L26/008A61L26/009A61L26/0066A61L26/0023C08J2301/28A61L2300/236A61L2300/404A61L2300/418A61L2300/604C08L1/28
Inventor 张培华李瑾范先谋
Owner 张 培华
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