Bifidobacterium longum subsp.infantis for relieving colitis and application thereof

A Bifidobacterium longum, infant technology, applied in the field of microorganisms, can solve side effects and other problems, achieve the effect of reducing colon shortening, reducing disease activity index, and broad market prospects

Pending Publication Date: 2022-06-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional treatment drugs currently used are: sulfasalazine salicylic acid preparations such as mesalamine, etc.; corticosteroids commonly u...

Method used

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  • Bifidobacterium longum subsp.infantis for relieving colitis and application thereof
  • Bifidobacterium longum subsp.infantis for relieving colitis and application thereof
  • Bifidobacterium longum subsp.infantis for relieving colitis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Screening, identification, culture, observation and preservation of B. longum subsp. infantis CCFM1210

[0080] 1. Filter

[0081] Take 0.5 g of healthy infant stool samples stored in 30% (v / v) glycerol from Yangzhou, Jiangsu, and spread them on MRS solid medium (containing 0.5 g / L cysteine) after gradient dilution. 37 Anaerobic culture at ℃ for 72h, and the colony morphology was observed and recorded; the colonies with milky white, smooth surface and round protrusions were picked and streaked on MRS solid medium (containing 0.5g / L cysteine), and anaerobic at 37 ℃ Purification and cultivation were carried out under the conditions of 0.5 g / L cysteine, and this operation was repeated 3 times to obtain purified single colonies; the purified colonies were picked and inoculated into MRS liquid medium (containing 0.5 g / L cysteine), and anaerobic culture was carried out at 37 °C for 36 h. The strain CCFM1210 was obtained.

[0082] 2. Identification

[0083] Extra...

Embodiment 2

[0086] Example 2: Preparation of Bifidobacterium longum infantum subsp. CCFM1210 bacterial suspension

[0087] (1) Dip the bacterial liquid of Bifidobacterium longum infantis subsp. CCFM1210 from the cryopreservation tube and streak it on the MRS solid medium, and culture it anaerobically at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it into the MRS liquid culture In the medium, anaerobic culture at 37°C for 36h to obtain the activation solution; the activation solution was added to the MRS liquid medium according to the inoculum amount of 1% (v / v), anaerobic culture at 37°C for 24h, and the activation culture was repeated 3 times. The activated bacterial liquid was obtained.

[0088] (2) The activated bacterial liquid obtained in step (1) is inoculated into the MRS liquid medium according to the inoculum amount of 1% (v / v), cultured at 37° C. for 24 hours to obtain a fermentation liquid, and the fermentation liquid is centrifuged to collec...

Embodiment 3

[0090] Example 3: Relief effect of Bifidobacterium longum infantum subsp. CCFM1210 on symptoms of DSS-induced colitis in mice

[0091] The modeling method of DSS-induced colitis in mice and the treatment steps of Bifidobacterium longum infantum subspecies intervention are as follows:

[0092] (1) Preparation of 2.7% dextran sodium sulfate (DSS) solution: DSS was prepared into a 2.7% (w / v) dextran sodium sulfate (DSS) solution with sterile water.

[0093] (2) Forty 6-week-old healthy male C57BL / 6N mice were randomly divided into 5 groups with 8 mice in each group. The 5 groups were: blank control group, DSS group, and Bifidobacterium longum infantis subspecies ATCC15697 intervention group. (ATCC15697+DSS), Bifidobacterium longum infantum subsp. CCFM1210 intervention group (CCFM1210+DSS) and Bifidobacterium longum infantum subsp. FJND2M2 intervention group (FJND2M2+DSS), among which Bifidobacterium longum infantum subsp. The bacterial suspension was prepared according to the me...

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Abstract

The invention discloses bifidobacterium longum subsp.infantis for relieving colitis and application, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1210 provided by the invention has the immunoregulation capability, and can improve the intestinal barrier and relieve colitis. Animal experiments prove that through intervention of the bifidobacterium longum subsp.infantis CCFM1210, tissue damage of a mouse can be remarkably relieved, the myeloperoxidase level can be remarkably reduced, the content of tight junction protein in colon tissue can be remarkably increased, the levels of proinflammatory cytokines IL-1beta and IL-6 can be remarkably reduced, and the level of an anti-inflammatory cytokine IL-10 can be remarkably increased; the concentration of butyric acid is obviously increased, the diversity of intestinal flora is obviously improved, and the disorder of the flora is relieved. Compared with a traditional medicine for treating colitis, a medicine or a probiotic preparation prepared from the bifidobacterium longum subsp. Infantis CCFM1210 disclosed by the invention has certain advantages and has a wide market prospect.

Description

technical field [0001] The invention relates to a Bifidobacterium longum infantis subspecies for relieving colitis and its application, and belongs to the technical field of microorganisms. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease. Ulcerative colitis (UC) is one of the main forms of IBD, which is mainly characterized by weakened intestinal barrier function and intestinal immune system dysregulation. Patients with UC The most common symptoms are diarrhea, rectal bleeding, tenesmus, mucus discharge, and abdominal pain. The exact pathogenesis of ulcerative colitis is unknown, but several factors are thought to be involved, including a dysregulated immune response, altered gut microbiota, genetic susceptibility, and environmental factors. Ulcerative colitis directly affects the quality of life, and choosing an appropriate treatment method is crucial to improving the quality of life of patients with ulcerative co...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P1/00A61P1/04A23L33/135A23L2/38C12R1/01
CPCC12N1/20A61K35/745A61P1/00A61P1/04A23L33/135A23L2/382A23V2002/00A23V2400/533A23V2200/3204Y02A50/30
Inventor 杨波陈卫李明洁陈海琴刘小鸣崔树茂赵建新张灏
Owner JIANGNAN UNIV
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