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Burkholderia gladioli MEJ03 and application thereof

A technology of Burkholderia glabra, MEJ03, applied in Burkholderia gladiolus MEJ03 and its application field, can solve the problem of no degradation of phthalates and achieve the effect of promoting plant growth

Pending Publication Date: 2022-07-01
JIANGHAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been used in biological control, decomposing toxic substances and other fields. The existing Burkholderia gladiolus has not been found to have the effect of degrading phthalates

Method used

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  • Burkholderia gladioli MEJ03 and application thereof
  • Burkholderia gladioli MEJ03 and application thereof
  • Burkholderia gladioli MEJ03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and identification of Burkholderia gladioli strain MEJ03

[0029] The configuration of the medium is as follows: LB medium: yeast extract 5.0 g, tryptone 10.0 g, NaCl 10.0 g, add deionized water to 1.0 L, 121° C., 20 min, pH 7.0-7.2. MSM medium: KH 2 PO 4 0.5 g, K 2 HPO 4 0.5 g, CaCl 2 0.01 g, Fe 2 (SO 4 ) 3H 2 O 0.001 g, (NH 4 ) 2 SO 4 1.0g, MgSO 4 ·7H 2 O 0.2g, dilute to 1000mL with distilled water, pH7.0-7.2, sterilize at 121°C for 30min.

[0030] Bioaccumulation of DEHP-degrading bacteria: put 5g of the rhizosphere soil in the rice-wheat rotation area into a 250mL conical flask containing 50mL of MSM medium with 500mg / L DEHP as the sole carbon source, at 28°C, Incubate at 180 rpm for 5 days.

[0031] Preliminary screening: The soil solution after the above-mentioned cultivation was applied by gradient dilution on LB solid medium, and after culturing at 28°C for 5 days, single colonies with obvious differences in colony morphology...

Embodiment 2

[0038] Example 2 Effects of different temperatures and different initial pH on DEHP degradation by MEJ03

[0039] Pick a single colony of MEJ03, activate it overnight at 28°C, and inoculate it into 100 mL of MSM+Y medium containing 500 mg / L DEHP with 2% inoculum. The MSM+Y medium includes: add 1% (w) to the MSM medium. / v) Yeast extract.

[0040] Under different temperature and initial pH conditions, after culturing at 28 °C for 5 days, the samples were extracted with dichloromethane, and the samples were dissolved in methanol after rotary evaporation. Finally, the degradation rate of DEHP was determined by high performance liquid chromatography. The results are as follows Figure 4 As shown, MEJ03 degrades 500mg / L DEHP with high degradation rate (>50%) at 24℃-36℃ and initial pH of 5.0-9.0, the optimum degradation temperature is 28℃, and the optimum degradation initial pH is 7.0.

[0041] After testing, it was found that the degradation rate of this strain to 500mg / L phthala...

Embodiment 3

[0042] Example 3 Degradation kinetics of DEHP by MEJ03 under optimum degradation conditions

[0043] A single colony of MEJ03 was picked, activated at 28°C overnight, and then inoculated into 100 mL of MSM+Y medium containing 500 mg / L DEHP at 2% inoculum. Under the optimal degradation conditions, the degradation kinetics of MEJ03 to 500mg / L DEHP are as follows: Figure 5 As shown, with the increase of culture time, the degradation rate of MEJ03 to 500 mg / L DEHP increased continuously, and the degradation rate of MEJ03 to 500 mg / LDEHP could reach more than 94% after 4 days of culture.

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Abstract

The invention relates to the technical field of microorganisms, in particular to burkholderia gladioli MEJ03 and application thereof. The preservation number of the burkholderia gladioli MEJ03 is CCTCC (China Center for Type Culture Collection) NO: M20211229. The burkholderia gladioli MEJ03 can be used for efficiently degrading phthalic acid ester, especially di (2-ethylhexyl) phthalate, has phosphorus dissolving activity, can be used for dissolving insoluble phosphate calcium phosphate and ground phosphate rock, and can also be used for promoting plant growth.

Description

technical field [0001] The present application relates to the field of microorganism technology, in particular to a strain of Burkholderia gladiolus MEJ03 and its application. Background technique [0002] Soil remediation by microbial degradation is a fast, effective and economical means of soil remediation. At present, PAEs-degrading strains have been isolated from various environments. The main bacteria include: bacteria Comamonas, Pseudomonas, Sphingomonas, Acinetobacter, Arthrobacter, Gordonella, Rhodococcus, Bacillus and other genera. Some fungal PAEs-degrading bacteria have also been isolated and identified, for example, the fungi Rhodotorula japonica, P. chrysosporium and Fusarium oxysporum and other genera. [0003] Phthalic acid esters (PAEs) include dimethyl phthalate (DMP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate A class of more than 30 compounds such as (DEHP). As plasticizers, they are commonly used in hundreds of products including cosmetic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00B09C1/10C05F11/08C05G3/80C12R1/01
CPCC12N1/20A01N63/20B09C1/10C05F11/08C05G3/80
Inventor 王慧敏孙孝文靳欣萌王璞
Owner JIANGHAN UNIVERSITY
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