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High-temperature-resistant degradation microbial inoculum for household kitchen garbage compost and method of high-temperature-resistant degradation microbial inoculum

A technology of kitchen waste and high temperature resistance, which is applied in the field of kitchen waste treatment and environmental microorganisms, can solve the problems of prolonging the duration of the high temperature period, accelerating the heating of the pile, and slow start-up heating, so as to achieve fast gelation, improve efficiency, The effect of high thermal stability

Active Publication Date: 2022-07-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the bottleneck problems such as slow start-up temperature rise, low fermentation temperature, long decomposing period, and poor product quality of household kitchen waste composting, the present invention provides a high-temperature-resistant degradation bacterial agent and its method for household kitchen waste composting, which can accelerate composting Body temperature rises, prolongs the duration of the high temperature period, and promotes the material to rot

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  • High-temperature-resistant degradation microbial inoculum for household kitchen garbage compost and method of high-temperature-resistant degradation microbial inoculum
  • High-temperature-resistant degradation microbial inoculum for household kitchen garbage compost and method of high-temperature-resistant degradation microbial inoculum
  • High-temperature-resistant degradation microbial inoculum for household kitchen garbage compost and method of high-temperature-resistant degradation microbial inoculum

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Embodiment 1

[0055] In the present embodiment, the gellan gum medium for separating and culturing high temperature resistance has been modified to overcome its shortcoming that it is easy to crack in a high temperature and water evaporation environment, and the details are as follows:

[0056] With 10.0~20.0g / L gellan gum+0~10mL 20mM CaCl 2 The solution or 10.0-20.0g / L agar was used as a coagulant respectively, and deionized water was added directly to prepare a solid medium, which was placed in an incubator at 80°C and 90°C for 3 days, and the shape change of the medium was observed to explore the best formulation of the coagulant. .

[0057] Comparing 10.0g / L gellan gum and 10.0g / L agar, no matter adding gellan gum or agar, the medium is softer than jelly after cooling and solidification, and it becomes liquid completely at less than 75℃. Indicate the need to increase the coagulant concentration and add 20mM CaCl 2 The solution acts as a thickener (hardener).

[0058] The addition of ...

Embodiment 2

[0066] In this embodiment, directional separation and screening of bacterial species that can be used for the preparation of high-temperature-resistant degrading bacterial agents for household kitchen waste composting are as follows:

[0067] (1) Environmental genome analysis of kitchen waste compost samples during high temperature period

[0068] DNA was extracted from the samples in the high temperature stage of aerobic composting of kitchen waste and amplified by PCR. According to the characteristics of the amplified 16S region, a small fragment library was constructed, and paired-end sequencing (Paired_End) was performed on the library based on the Illumina NovaSeq sequencing platform. After reads splicing and filtering, OTUs (Operational Taxonomic Units) clustering, species annotation and abundance analysis were performed. The results showed that at the phylum level, Firmicutes (42.18%), Actinobacteriota (25.45%) and Proteobacteria (11.84%) dominated the samples; at the ...

Embodiment 3

[0091] In this embodiment, a high-temperature-resistant degrading bacterial agent for household kitchen waste composting is prepared, as follows:

[0092] (1) Strain expansion culture

[0093] H-CD-1 (Aeribacillus sp.), H-CD-5 (Pueribacillus sp.), H-CD-6 (Bacillus licheniformis), M-FW-7 (Bacillus subtilis) were inoculated into LB liquid medium ( pH 7.4, including 10.0g / L NaCl, 10.0g / L tryptone, 5.0g / L yeast extract), shaking culture at 55°C and 180rpm for 2d to obtain four bacterial liquids.

[0094] (2) Fermentation of solid compound bacterial agent

[0095] 2kg corncob (particle size 1-2cm) and 3kg bamboo chips (passed through a 4-mesh sieve) were sterilized by high pressure steam at 121°C for 30min and mixed evenly, and 250g cornstarch, 250g soybean meal, 25g (NH4) were added. 4 ) 2 SO 4 , 25g KH2 PO 4 , 5g NaCl, 5g MgSO 4 ·7H 2 O, 0.5g MnSO 4 In the solid matrix as nutrients, H-CD-1 (Aeribacillus sp.), H-CD-5 (Pueribacillus sp.), H-CD-6 (Bacillus licheniformis), M-...

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Abstract

The invention discloses a high-temperature-resistant degradation bacterial agent for household kitchen waste composting and a method of the high-temperature-resistant degradation bacterial agent. The high-temperature-resistant degradation bacterial agent is prepared from Aeribacillus sp., Pueribacillus sp., bacillus licheniformis and bacillus subtilis, and the high-temperature-resistant degradation bacterial agent is prepared from the following raw materials of bacillus licheniformis, bacillus subtilis and bacillus subtilis. The high-temperature-resistant degradation bacterial agent is formed by compounding four high-temperature-resistant synergistic bacteria which are obtained by directionally separating and screening a novel gellan gum culture medium and respectively have high starch, protein, fat and cellulose degradation capacities, the effective viable count of the solid composite bacterial agent obtained by fermentation can reach 2.87 * 10 < 11 > cfu / g, the water content is less than or equal to 30%, and the high-temperature-resistant degradation bacterial agent is convenient for long-time household storage; by applying the microbial agent, the quantity and activity of thermophilic microorganisms in a composting system can be improved, and the bottleneck problems of slow starting temperature rise, low fermentation temperature, long decomposition period, poor product quality and the like of household kitchen garbage composting are effectively solved.

Description

technical field [0001] The invention relates to a high-temperature-resistant degrading bacterial agent for household kitchen waste composting and a method thereof, which are used to realize rapid reduction, resource utilization and harmlessness of household kitchen waste composting, and belong to kitchen waste treatment and environmental microorganisms. technical field. Background technique [0002] In the post-epidemic era, household composting has gradually entered the Chinese market from Europe under the background of the garbage classification boom and the "dual carbon". However, in practical applications, due to the lack of mechanical control systems in self-made equipment or the lack of composting experience by users, household kitchen waste composting usually has problems such as slow start-up temperature rise, low fermentation temperature, long decomposing period, and poor product quality, which dissuade users from participating in composting. . Therefore, technolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F9/04C12R1/125C12R1/10C12R1/01
CPCC12N1/20C05F9/04Y02W30/40
Inventor 吴伟祥莫洁菲赵昶勋阮诗婷喻琛越张家玮汪博琛
Owner ZHEJIANG UNIV