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Cryopreservation liquid and preparation method and application thereof

A cryopreservation solution and solution technology, applied in the field of cryopreservation solution, can solve the problems of stem cell oxidative damage and differentiation potential, high freezing rate requirements, high cell mass rate, etc. group, the effect of improving survival rate

Pending Publication Date: 2022-07-19
达瑟儿(上海)生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Permeable cryoprotectants are generally small molecular substances, such as glycerin, dimethyl sulfoxide (DMSO), ethylene glycol, propylene glycol, methanol, ethanol, formamide, etc., whose main function is to prevent the formation of ice crystals in cells and avoid high The toxicity of the concentration cryopreservation agent and the dehydration of the cells within tolerance, the permeable cryoprotectant is more commonly used methyl sulfoxide (DMSO), but dimethyl sulfoxide (DMSO) is toxic, when the amount Too much will damage the cells and easily lead to oxidative damage and reduced differentiation potential of stem cells during recovery
Non-permeable cryoprotectants are mainly some macromolecular substances, such as trehalose, sucrose, glucose, mannitol, sorbitol, hydroxyethyl starch (HES) and polyvinylpyrrolidone, etc., and their requirements on the freezing rate during cryopreservation Very high, the use of macromolecular substances will make the rate of cell clusters too high and bring great risks to patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 of the present invention provides a cryopreservation solution, the cryopreservation solution includes the following components, in parts by volume, 24 parts of human albumin solution, 5 parts of DMSO, 30 parts of mixed glucose injection, serum-free basis 40 copies of culture medium;

[0054] The volume concentration of the human serum albumin solution refers to 20%;

[0055] The mixed glucose injection is dextran 40 glucose injection and glucose sodium chloride injection; the volume ratio of the dextran 40 glucose injection and glucose sodium chloride injection is 2:1;

[0056] The components of the freezing solution further include licochalone; the weight of the licoricin is 0.5% of the weight of the freezing solution; the licoricone is licochalone A;

[0057] The components of the cryopreservation solution also include 20-glucosyl-ginsenoside Rf; the weight ratio of the 20-glucosyl-ginsenoside Rf to licochalone is 1.5:1;

[0058] The preparation method ...

Embodiment 2

[0061] Embodiment 2 of the present invention provides a cryopreservation solution, the cryopreservation solution includes the following components, in parts by volume, 28 parts of human albumin solution, 7 parts of DMSO, 5 parts of mixed glucose injection, serum-free basis 45 copies of culture medium;

[0062] The volume concentration of the human serum albumin solution refers to 20%;

[0063] The mixed glucose injection is dextran 40 glucose injection and glucose sodium chloride injection; the volume ratio of the dextran 40 glucose injection and glucose sodium chloride injection is 4:1;

[0064] The components of the freezing solution also include licochalone; the weight of the licochalone is 1% of the weight of the freezing solution; the licochalone is licochalone A;

[0065] The components of the cryopreservation solution also include 20-glucosyl-ginsenoside Rf; the weight ratio of the 20-glucosyl-ginsenoside Rf to licochalone is 1.5:1;

[0066] The preparation method of ...

Embodiment 3

[0069] Embodiment 3 of the present invention provides a cryopreservation solution, the cryopreservation solution includes the following components, in parts by volume, 25 parts of human albumin solution, 6 parts of DMSO, 32 parts of mixed glucose injection, serum-free basis 42 copies of culture medium;

[0070] The volume concentration of the human serum albumin solution refers to 20%;

[0071] The mixed glucose injection is dextran 40 glucose injection and glucose sodium chloride injection; the volume ratio of the dextran 40 glucose injection and glucose sodium chloride injection is 3:1;

[0072] The components of the freezing solution also include licorice chalcone; the weight of the licorice chalcone is 0.7% of the weight of the freezing solution; the licorice chalcone is licorice chalcone A;

[0073] The components of the cryopreservation solution also include 20-glucosyl-ginsenoside Rf; the weight ratio of the 20-glucosyl-ginsenoside Rf to licochalone is 1.8:1;

[0074]...

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Abstract

The invention relates to the technical field of biology, in particular to a cryopreservation solution and a preparation method and application of the cryopreservation solution. The cryopreservation liquid comprises the following components: a human serum albumin solution, DMSO, a mixed glucose injection and a serum-free basic culture medium. According to the cryopreservation liquid, on one hand, redundant blood components in cells can be agglutinated, the cells are prevented from clotting, and the aim of keeping the cells for a long time is achieved, on the other hand, the clotting rate of the recovered cells is low, and the cryopreservation liquid has high safety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cryopreservation solution, a preparation method and application of the cryopreservation solution. Background technique [0002] Mesenchymal stem cells (MSCs), an important member of the stem cell family, originate from the mesoderm and ectoderm in the early stage of development. Mesenchymal stem cells can be expanded in large quantities and are widely used in clinical and scientific research. The expanded stem cells sometimes need to be cryopreserved. The purpose is to slow down the metabolic activity of cells and maintain life in a low temperature environment. , for use in scientific research and clinical recovery at any time. At present, cryopreservation solution needs to be added to the cryopreservation of mesenchymal stem cells. [0003] Cryoprotectants can be divided into two types, permeable and non-permeable, according to whether they penetrate cell membranes. Permeable c...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 朱灏葛晨曦张钰吴丹枫
Owner 达瑟儿(上海)生命科技有限公司
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