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Method for preparing lactic acid and feedstuff concurrent with crop straw fermentation

A technology of crop straw and lactic acid, applied in the directions of microorganism-based methods, biochemical equipment and methods, animal feed, etc., can solve problems such as inability to produce feed

Inactive Publication Date: 2007-03-14
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods can only produce lactic acid, but cannot produce lactic acid and feed at the same time, especially the use of crop straw to produce lactic acid and produce feed at the same time

Method used

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  • Method for preparing lactic acid and feedstuff concurrent with crop straw fermentation
  • Method for preparing lactic acid and feedstuff concurrent with crop straw fermentation

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Experimental program
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Effect test

specific Embodiment approach 1

[0005] Specific embodiment 1: (see Fig. 1, Fig. 2) this embodiment is realized according to the following steps: (1) crop stalks pretreatment: After the stalks are crushed to 80-140 meshes, soak in dilute lye for 12-36 hours , filtered, and washed until the washing liquid is neutral. The waste liquid produced by pretreatment is recovered lignin and lye by bipolar membrane electrodialysis. (2) Preparation of cellulase koji by solid-state fermentation: Trichoderma and fungi producing cellulase are selected as starting strains, and solid-state fermentation is carried out at 25-30°C for 60-108 hours, and the cellulase koji produced by fermentation is directly used Subsequent simultaneous fermentation. The specific steps for preparing cellulase koji are as follows: a. Inoculate the cellulase-producing bacterial strain into the slant medium for culture, scrape a few ring spores from the activated slant and insert them into sterile water with glass beads, shake and disperse the spor...

specific Embodiment approach 2

[0006] Specific embodiment two: (referring to Fig. 1, Fig. 2) present embodiment is realized according to the following steps: (1) after bean stalk is pulverized to 140 orders, soak 24 hours with the ammoniacal liquor of 10% concentration at room temperature, filter, wash to The washing liquid is neutral. The waste liquid produced by pretreatment is separated from lignin and lye by bipolar membrane electrodialysis. (2) Trichoderma konshii is inoculated into the slant medium and cultivated until the mycelia grow vigorously, scrape a few rings of spores from the slant and insert them into sterile water with glass beads, vibrate, disperse the spores, measure the spore concentration, and make the spore suspension concentration reach 10 7 individual / mL. (3) Add straw powder, bran, acetic acid-sodium acetate buffer solution, etc. to the container to prepare a culture medium, so that the weight ratio of straw powder and bran is 1:1, the solid-liquid weight ratio is 2:5, and the ace...

specific Embodiment approach 3

[0008]Specific embodiment three: (referring to Fig. 1, Fig. 2) present embodiment is realized according to the following steps: (1) corn stalk is pulverized, crosses 80 mesh sieves, soaks 24 hours with the ammoniacal liquor of 8% concentration under room temperature, solid-liquid The ratio is 1:10, then filtered and washed until the washing liquid is neutral. The waste liquid produced by pretreatment is separated from lignin and lye by bipolar membrane electrodialysis. (2) Trichoderma konshii is inoculated into the slant medium and cultivated until the mycelia grow vigorously, scrape a few rings of spores from the slant and insert them into sterile water with glass beads, vibrate, disperse the spores, measure the spore concentration, and make the spore suspension concentration reach 10 7 individual / mL. (3) Add pretreated straw powder, bran, water, and ammonium sulfate in the container, so that the weight ratio of straw powder and bran is 1:1, the solid-liquid weight ratio is...

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Abstract

The invention relates to crop stalk fermentation and lactic acid and quality fodder preparation method. The synchronous diastatic fermentation provided by the invention is: adding in pretreated stalk of 2-3 times water in weight, 121 DEG C sterilizing, adding cellulase leaven (step 2 prepared), enzyme activity of cellulase leaven of total substrate amounting to 42-50IU / g in cellulose filter paper enzyme activity, inoculating activated high temperature resistant lactobacillus, inoculating volume is 5-10% of total substrate, fermenting 72-108 hours in 45-50 DEG C condition, adjusting substrate pH to 5.0-6.0 during fermenting. The invention shortens greatly of fermentation time, promotes the fermentation effiency. The 72-108 hours synchronous fermentation can produce 6-8g lactic acid and 60-70g quality fodder every 100g dry stalk. The invention has merits of low cost, low investment, no pollution, little energy consumption, high resource exploitation.

Description

Technical field: [0001] The invention relates to a method for simultaneously producing lactic acid and high-quality feed by fermenting crop stalks. Background technique: [0002] Lactic acid is not only widely used in the food industry and pharmaceutical industry, but also used as an industrial raw material for leather and fiber industries. Especially in recent years, as a biodegradable plastic, polylactic acid has many excellent properties, and the manufacture of its raw material lactic acid has attracted more attention. In the past, the raw materials used for lactic acid fermentation were generally starches from crops such as corn, cassava, wheat, and potatoes. But these crops are not only ideal raw materials for lactic acid fermentation, but also valuable food. From the perspective of efficient resource utilization and cost reduction of lactic acid production, the use of other cheap carbon sources for fermentation is one of the research hotspots in lactic acid productio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56A23K1/14C12R1/645A23K10/12A23K10/37
CPCY02P60/87
Inventor 汪群慧汲永臻孙晓红徐忠
Owner HARBIN INST OF TECH
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