Method for promoting terpene indole alkaloid content of Vinca rosea hairly root by transferring g10h gene

Abstract

The invention discloses a Vinca rosea trichoid root terpenes indole alkaloid content improving method of trans-g10h gene in the biological domain, which is characterized by the following: cloning g10h gene form vinca rosea; constructing plant expression carrier of g10h gene; gaining trans-gene Vinca rosea trihoid root by genetic conversing Vinca rosea; measuring the alkaloid content of Vinca rosea trichoid root by HPLC; measuring relative gene expression of biological synthesis of Vinca rosea trichoid root alkaloid by fluorescence quantitative PCR. The important alkaloid content of trans-gene Vinca rosea trihoid root from the invention gets a distinctive increasing and provides a new method of producing anti-cancer drugs VLB and LCR for clinic demand.

Description

technical field

[0001] The invention relates to a method in the field of biotechnology, in particular to a method for transforming a geraniol 10-decarboxylase (G10H) gene to improve the content of vinca hairy root terpene indole alkaloids (TIAs). Background technique

[0002] Vinca (Catharanthus roseus) is a plant of the genus Vinca in the family Apocynaceae. At present, more than 100 terpenoid indolealkaloids (Terpenoid indolealkaloids, TIAs) have been isolated from the whole plant of Vinca, such as Vinblastine (Vinblastine). ), Vincristine, Ajmalicine, Serpentine, Vindoline, Catharanthine, Lochneridine, Pivenli ( Perivine) and so on. Most TIAs are widely used in modern medicine due to their biological activity. For example, the famous antineoplastic drugs vinblastine and vincristine can be used to treat Hodgkin's disease, malignant lymphoma, acute lymphocytic leukemia, villous epithelial Cell carcinoma and other cancers, its sulfate has been widely used clinically, and i...