Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy

Inactive Publication Date: 2006-05-02
UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0023]The present invention relates to the design and construction of synthetic DNA molecules that encode one of the seven light chains of Clostridium botulinum neurotoxin and are capable of being expressed in heterologous prokaryotic or eukaryotic hosts. The invention is based, in part, on modif

Problems solved by technology

The toxins primarily act by inhibiting the neurotransmitter acetylcholine at the neuromuscular junction, causing paralysis.
The most common immediate cause of death is respiratory failure due to diaphragmatic paralysis.
Extreme toxicity of these toxins and their lability in purified preparations have limited any detailed characterizations.
Their proteolysis inhibits exocytosis and blocks acetylcholine secretion, leading ultimately to muscular paralysis.
However, in digitonin-permeabilized chromaffin cells, the LC inhibits exocytosis (Bittner et al., 1989), and direct microinjection of the LC into the cytosol results in blockage of membrane exocytosis (Bittner et al., 1989; Bi et al., 1995).
In spite of such immense importance, studies of the LC have been limited by its availability.
These preparations therefore retain some contaminating toxicity of the dichain, have low solubility, and often begin to proteolytically degrade and start losing activity within hours of storage in solution.
The LC of serotype A has been separated and purified from the full-length toxin by QAE-Sephadex chromatography from 2 M urea; however, the preparation suffers from low solubility (Shone and Tranter, 1995).
These preparations almost invariably contain contaminating full-length toxins, and the commercially available preparations precipitate from solution or undergo proteolytic degradation upon hours of storage in solution.
However, the poor expression of the cloned products, probably due to rare codon usage in clostridial DNA (Makoff et al., 1989, Winkler and Wood, 1988), remained a major hurdle in obtaining adequate amount of the protein for structural and functional studies.
Inactivation of SNAP-25, VAMP, or syntaxin by BoNT leads to an inability of the nerve cells to release acetylcholine resulting in neuromuscular paralysis and possible death, if the condition remains untreated.
However, recombinant methods as described in the publications above do not yield optimal results because botulinum codons are not translated well in other organisms commonly used for production, such as E. coli or yeast.
Furthermore, no easily translatable, recombinant form of the non-neurotoxic, mutated light chain presently exists.
These preparations, therefore, retain some contaminating toxicity, have low solubility, and undergo proteolytic degradation within hours and days of storage in solution.
The poor expression of the native gene was probably due to the high A+T composition found in the clostridial DNA.

Method used

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  • Recombinant light chains of <i>botulinum</i> neurotoxins and light chain fusion proteins for use in research and clinical therapy
  • Recombinant light chains of <i>botulinum</i> neurotoxins and light chain fusion proteins for use in research and clinical therapy
  • Recombinant light chains of <i>botulinum</i> neurotoxins and light chain fusion proteins for use in research and clinical therapy

Examples

Experimental program
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Effect test

example 1

[0068]Chemicals, Buffers, and Reagents

[0069]Buffer T (20 mM Tris-HCl, pH 9.2) and buffer G (50 mM sodium glycine, pH 9.0) were used as indicated. SKL (sodium N-lauryl sarcosine or sarkosyl) was from Sigma. Highly purified (>95%) full-length BoNT / A was purchased from List Biologicals (Campbell, Calif.). Rabbit polyclonal antibodies against a 16-residue N-terminal sequence (PFVNKQFNYKDPVNGV; SEQ ID NO:1) of the BoNT / A LC were produced and affinity purified by Research Genetics (Huntsville, Ala.). Peroxidase-coupled goat anti-rabbit and anti-mouse IgG (H+L) and ABTS substrate were from Kirkegaard Perry Laboratories (Gaithersburg, Md.). Oligonucleotides, designed for E. coli codon usage (Anderson and Kurland, 1990) and ranging in size from 70 to 100 nucleotides, were synthesized by Macromolecular Resources (Fort Collins, Colo.).

example 2

[0070]Construction and Expression of a Synthetic DNA Encoding rBoNT / A LC

[0071]The DNA encoding the enzymatic LC domain of BoNT / A was assembled from three segments, a 335-base pair (bp) Sal I-Sph I fragment, a 600-bp Sph I-Kpn I fragment, and a 460-bp Kpn I EcoR I fragment. To construct the first segment, six oligonucleotide pairs were annealed, ligated, and, after PCR amplification, inserted into pGEM3Zf at Sal I-Sph I restriction enzyme sites. The second segment was built by annealing and ligating eight oligonucleotide pairs, followed by its amplification and insertion into the Sph I and Kpn I sites of pGEM3Zf. The final segment was constructed by annealing and ligating six oligonucleotide pairs, followed by its amplification and insertion into the Kpn I-EcoR I sites of pGEM3Zf. Nucleotide sequencing of gene fragments in pGEM3Zf was performed to identify clones in each group with minimal misincorporations. In vitro mutagenesis was performed to correct the misincorporations in the B...

example 4

[0079]Extraction and Purification of Light Chain as Inclusion Bodies

[0080]In a typical preparation, 12 g of E. coli cells was suspended in a total volume of 30 ml of buffer T containing 5 mM MgCl2, 1.5 mM PMSF, 10 mM β-mercaptoethanol, and 2 mg of DNAse. The cell suspension was subjected to 10 cycles of 2-min sonication (at 60% power in a Fisher Model 300 Sonic Dismembrator) and 2-min cooling on ice. After centrifugation for 15 min at 10,000 ×g, the supernatant was discarded. The pellet was suspended in 30 ml the above buffer. The cycle of sonication and centrifugation was repeated five more times; MgCl2 and DNAse were omitted from the buffer during the last two cycles. The resulting pellet contained the rBoNT / A LC, that appeared ˜70% pure by SDS PAGE (FIG. 2). The pellet was stored at 4° C. as a white suspension in 15 ml of buffer T containing 1.5 mM PMSF and 10 mM β-mercaptoethanol.

[0081]Results

[0082]The expressed LC appeared exclusively in the insoluble pellet fraction (FIG. 2). ...

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Abstract

Botulinum neurotoxins, the most potent of all toxins, induce lethal neuromuscular paralysis by inhibiting exocytosis at the neuromuscular junction. The light chains (LC) of these dichain neurotoxins are a new class of zinc-endopeptidases that specifically cleave the synaptosomal proteins, SNAP-25, VAMP, or syntaxin at discrete sites. The present invention relates to the construction, expression, purification, and use of synthetic or recombinant botulinum neutoroxin genes. For example, a synthetic gene for the LC of the botulinum neurotoxin serotype A (BoNT / A) was constructed and overexpressed in Escherichia coli. The gene product was purified from inclusion bodies. The methods of the invention can provide 1.1 g of the LC per liter of culture. The LC product was stable in solution at 4° C. for at least 6 months. This rBoNT / A LC was proteolytically active, specifically cleaving the Glu-Arg bond in a 17-residue synthetic peptide of SNAP-25, the reported cleavage site of BoNT / A. Its calculated catalytic efficiency kcat / Km was higher than that reported for the native BoNT / A dichain. Treating the rBoNT / A LC with mercuric compounds completely abolished its activity, most probably by modifying the cysteine-164 residue located in the vicinity of the active site. About 70% activity of the LC was restored by adding Zn2+ to a Zn2+-free, apo-LC preparation. The LC was nontoxic to mice and failed to elicit neutralizing epitope(s) when the animals were vaccinated with this protein. In addition, injecting rBoNT / A LC into sea urchin eggs inhibited exocytosis-dependent plasma membrane resealing.

Description

[0001]This application is a continuation-in-part of U.S. patent application Ser. No. 09 / 910,186 filed Jul. 20, 2001, which is a continuation of U.S. patent application Ser. No. 09 / 611,419 filed Jul. 6, 2000, which is a continuation of U.S. patent application Ser. No. 08 / 123,975, filed Sep. 21, 1993, now abandoned, wherein said application 09 / 611,419 is based on U.S. Provisional Applications Nos. 60 / 133,866, 60 / 133,868, 60 / 133,869, 60 / 133,865, 60 / 133,873, and 60 / 133,867, all filed May 12, 1999, all of which are incorporated herein by reference in their entirety. The instant application is also based on U.S. Provisional Application No. 60 / 246,774, filed on Nov. 6, 2000, and U.S. Provisional Application No. 60 / 311,966 filed Aug. 6, 2001, which are incorporated herein in their entirety by reference.FIELD OF THE INVENTION[0002]The invention is directed to construction, expression, and purification of synthetic DNA molecules encoding polypeptides comprising botulinum neutrotoxin (BoNT) li...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N1/12C12N1/20C12N15/09C12P21/04A61K39/00A61K39/08C07K14/33C12N1/21C12N9/64
CPCA61K39/08C12N9/52C12N9/6489C07K14/33A61K39/00Y10S435/842
InventorSMITH, LEONARD A.JENSEN, MELODY
OwnerUNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY