The present invention relates to
analytical testing devices comprising segmented
fluidics and methods for assaying coagulation in a fluid sample received within the segmented
fluidics. For example, the present invention may be directed to sample analysis
cartridge including an inlet chamber, a first conduit comprising a first junction configured to split a biological sample into at least first and second segments, a second conduit comprising a first
reagent, a first sensor region, and a first fluidic lock valve, and a third conduit comprising a first flow restrictor region, a second
reagent, and a second sensor region. The sample analysis
cartridge further includes a pump configured to independently mix the first segment in the second conduit and the second segment in the third conduit, and independently position the first segment over the first sensor region and position the second segment over the second sensor region.