Prepn process of collagen-based surface wound repairing membrane possessing tissue induction
A wound repair and inductive technology, applied in bandages, absorbent pads, medical science and other directions, can solve the problems of thin and fragile membranes, lack of dermal components, poor mechanical properties, etc., to avoid scarring, shorten wound healing time, Well-integrated effect
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[0027] Example 1:
[0028] 1. Preparation of sustained-release microspheres
[0029] Place 50 parts of castor oil containing 1% Tween-80 in a three-necked bottle, preheat to 50°C, and add 5 parts of a gelatin:chitosan=9:1 mixed solution with a total concentration of 5% under stirring. Stir for 10 minutes to form a water-in-oil emulsion, switch to an ice bath, continue stirring for 10 minutes, add 30 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 3 parts of 1% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000 rpm, 15 min, 4° C.), and dried in air. Then the gelatin microspheres were immersed in a solution containing 0.25% glutaraldehyde and 1% Tween-80, stirred for 12 hours at a temperature of 4°C, and centrifuged. The resulting microspheres were placed in a solution containing 10mM glycine and 1% Tween-80. In the solution, at a temp...
Example Embodiment
[0032] Example 2:
[0033] 1. Preparation of sustained-release microspheres
[0034] Put 80 parts of liquid paraffin containing 2% Span-80 in a three-necked flask, preheat to 50°C, add 8 parts of a total concentration of 8% gelatin: collagen = 8: 2 mixed solution under stirring, and stir to form oil After the water-in-water emulsion was changed to an ice bath, the stirring was continued for 10 minutes. Add 40 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 2 parts of 3% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based composite microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000 rpm, 15 min, 4° C.), and dried in air to obtain uncrosslinked microspheres. The uncrosslinked gelatin-based composite microspheres were immersed in a solution containing 0.5% glutaraldehyde and 1% Span-80, stirred for 12 hours at a temperature of 4°C, and centrifuged. The resulting microspheres were placed c...
Example Embodiment
[0037] Example 3:
[0038] 1. Preparation of sustained-release microspheres
[0039] Put 100 parts of olive oil containing 3% Span-80 in a three-necked bottle, preheat to 50°C, add dropwise a mixed solution of gelatin: collagen: chitosan = 8:1:1 with a total concentration of 10% under stirring 20 parts, stir to form a water-in-oil emulsion, switch to an ice bath, and continue to stir for 10 minutes. Add 50 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 1 part of 5% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based composite microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000 rpm, 15 min, 4° C.), and dried in air to obtain uncrosslinked microspheres. The uncrosslinked gelatin-based composite microspheres were immersed in a solution containing 1% glutaraldehyde and 1% Span-80, stirred for 12 hours at a temperature of 4°C, and centrifuged. The resulting microspheres were placed with 50...
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