Oxidation resistance zymolyte rich in pollen active peptide

A technology of antioxidant enzymes and pollen activity, applied in the fields of peptides, food science, hydrolyzed protein components, etc., can solve the problems of poor food palatability and insignificant biological efficacy, and achieve the effects of high solubility, good application development prospects and wide sources.

Inactive Publication Date: 2007-10-24
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to solve the problems of poor palatability of existing bee pollen, allergic reactions such as stomach pains in some people after taking bee pollen, and insignificant biological efficacy after taking bee pollen in small doses and for a short time; to provide a Antioxidant enzyme hydrolyzate rich in pollen active peptides derived from bee pollen

Method used

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  • Oxidation resistance zymolyte rich in pollen active peptide
  • Oxidation resistance zymolyte rich in pollen active peptide
  • Oxidation resistance zymolyte rich in pollen active peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Preparation of enzymatic hydrolyzate: Weigh a certain amount of broken wall rape bee pollen, add it to the buffer system with a pH value of 9 at a ratio of material to water of 1:10, mix well, adjust the temperature to 50°C, Add alkaline protease to hydrolyze at 1500U / g. After reacting for 3 hours, inactivate the enzyme at 90°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, remove macromolecular compounds such as proteins from the supernatant with an ultrafiltration membrane with a relative molecular weight cut-off > 10000 D, and filter the membrane permeated part (that is, the small molecular compound part) with a cut-off filter. The ultrafiltration or nanofiltration membrane with a relative molecular weight <400D removes monosaccharides, amino acids, ionic components, etc., and the filtrate is freeze-dried. After testing, after rape bee pollen was hydrolyzed by alkaline protease and membrane separated, the content of free nitrogen in the water extract of the or...

Embodiment 2

[0026] Preparation of enzymatic hydrolyzate: Weigh a certain amount of broken wall rape bee pollen, add it to the buffer system with a pH value of 6.5 at a material-to-water ratio of 1:10, mix well, adjust the temperature to 50°C, and set the ratio of enzyme to substrate concentration as Add papain at 10000U / g for hydrolysis. After reacting for 3 hours, inactivate the enzyme at 90°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, remove macromolecular compounds such as proteins from the supernatant with an ultrafiltration membrane with a relative molecular weight cut-off > 10000 D, and filter the membrane permeated part (that is, the small molecular compound part) with a cut-off filter. The ultrafiltration or nanofiltration membrane with a relative molecular weight <400D removes monosaccharides, amino acids, ionic components, etc., and the filtrate is freeze-dried.

Embodiment 3

[0028] Preparation of enzymatic hydrolyzate: Weigh a certain amount of broken wall rape bee pollen, add it to the buffer system with a pH value of 7 at a material-to-water ratio of 1:10, mix well, adjust the temperature to 40°C, and adjust the concentration ratio of enzyme to substrate to 800U / g Add neutral protease for hydrolysis. After reacting for 3 hours, inactivate the enzyme at 90°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, remove macromolecular compounds such as proteins from the supernatant with an ultrafiltration membrane with a relative molecular weight cut-off > 10000 D, and filter the membrane permeated part (that is, the small molecular compound part) with a cut-off filter. The ultrafiltration or nanofiltration membrane with a relative molecular weight <400D removes monosaccharides, amino acids, ionic components, etc., and the filtrate is freeze-dried.

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Abstract

The invention discloses an oxidation resistance enzymolysis substance containing a pollen active peptide, which comprises the following steps: breaking the bee pollen wall; adding 8-25 times deionized water after defatting; adjusting pH to 6-10; heating at 30-60 DEG C; adding to prolease in order to the enzymolysis reaction; controlling the temperature at 20-80 DEG C; mixing 30-180 min in the insulation condition; heating quickly at more than 80 DEG C, when the enzymolysis reaction gets to the reduction of the protein content; mixing 10-30 min in the insulation condition; stopping the enzymolysis reaction; reducing the ambient temperature quickly; separating the supernatant from the solid-liquid; separating with the hyperfiltration membrane of intercepting the relative molecular mass > 10000D; separating the filtering solution with the hyperfiltration membrane and the nano filtration membrane of intercepting the relative molecular mass < 400D; drying the filtering liquor. The invention acquires the function of oxidation resistance, which develops the potentiality greatly and applies the prospect well.

Description

technical field [0001] The invention relates to a bee pollen enzymatic hydrolyzate and a preparation method thereof. The enzymatic hydrolyzate has the characteristics of significantly improving the antioxidant capacity of bee pollen and is rich in pollen active peptides. The invention belongs to the technical field of deep processing of natural products and bioengineering, and specifically relates to biopharmaceutical technology or functional food processing technology, in particular to a method for preparing antioxidative enzymatic hydrolyzates rich in pollen active peptides from bee pollen. Background technique [0002] Bee pollen is the pollen collected by bees. In addition to the general pollen components, it also contains a small amount of nectar and secretions added during collection. It has strong physiological activities and can enhance human immune function, anti-tumor, anti-constipation, It delays aging and has a cosmetic effect, and is an ideal natural nutrient wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C12P21/06A23J3/34A61K38/01
Inventor 孙丽萍张智武田文礼董捷朱晓丽
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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