Coating micro-pill phytase and preparation method thereof
A technology of coated pellets and phytase, applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the unmentioned pellets enzyme solubility stability affinity, coating rate, product and environmental pollution , slow function and other issues, to achieve the effect of enhancing the ability of adverse environment, reducing impact and low cost
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Embodiment 1
[0050] With 130kg cornstarch, 60kg corncob powder, 40kg marble powder, 70kg lactose, a total of 300kg of materials were mixed in a mixer for 5 minutes, then 240kg of phytase fermentation liquid was added, (this fermentation liquid was produced by the phytase strain after Submerged liquid fermentation, using glucose, ammonium sulfate and other cultures to produce liquid fermentation products, the main components are water and phytase protein, the enzyme activity is 10000U / g), and continue to mix for 10 minutes.
[0051] Put the mixed material into the hopper of the swing granulator, start the equipment, and make the material into columnar granules with a length of about 3-5mm and a diameter of about 1-3mm. Put the columnar granular material into the inner cavity of the spherical rounder, start the equipment, adjust the rotation speed to 400-600rpm, and discharge the material until the columnar particles become round particles.
[0052] Put 300kg of round granular materials into...
Embodiment 2
[0055] With 100kg cornstarch, 40kg corncob powder, 20kg marble powder, 40kg lactose, 200kg in total were mixed in mixer for 5min, then added 300kg fermented liquid (this fermented liquid is produced phytase bacterial strain through liquid submerged fermentation, utilizes Glucose, ammonium sulfate and other cultures, the liquid fermentation product produced, the main components are water and phytase protein, the enzyme activity is 8000U / g), and continue to mix for 10 minutes.
[0056] Put the mixed material into the hopper of the swing granulator, start the equipment, and make the material into columnar granules with a length of about 3-5mm and a diameter of about 1-3mm. Put the columnar granular material into the inner cavity of the spherical rounder, start the equipment, adjust the rotation speed to 400-600rpm, and discharge the material until the columnar particles become round particles.
[0057] Put 300kg of round granular materials into the drying tower, start the equipme...
Embodiment 3
[0060] Performance Testing
[0061] 1. Appearance: Sensory measurement, using a magnifying glass to check the roundness and uniformity of the particles. The coated micropill phytase prepared by the invention is microspherical, round, regular in shape and uniform in particle size.
[0062] 2. Density: sensory measurement, no broken when twisted by hand.
[0063] 3. Enzyme activity uniformity: After a batch of products are produced, 10 samples are taken at different locations, and according to GB / T18634 Feed Phytase Activity Determination-Spectrophotometric Method, combined with the relevant guidelines of the national testing agency, The phytase activity of each sample was measured and then the coefficient of variation was determined. The coated micropill prepared by the invention has high uniformity of phytase activity content, and the coefficient of variation is less than 5%.
[0064] 4. Particle size: Use a standard sample sieve to randomly test the weight of 30-60 mesh pa...
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