Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Coating micro-pill phytase and preparation method thereof

A technology of coated pellets and phytase, applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the unmentioned pellets enzyme solubility stability affinity, coating rate, product and environmental pollution , slow function and other issues, to achieve the effect of enhancing the ability of adverse environment, reducing impact and low cost

Active Publication Date: 2010-06-02
北京昕大洋科技发展有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method improves the high temperature of the enzyme to withstand granulation and extrusion and maintain good enzyme activity in the feed processing process, it does not mention the solubility, stability at normal temperature, and Affinity with feed, coating rate, etc.
[0006] CN 1775067A discloses a production method of coated granule phytase. The coated granule phytase prepared by the method has good fluidity, dispersibility and temperature resistance, but the method uses an enteric coating material, which is difficult to obtain in the stomach. It is difficult to dissolve in the medium, and it can only dissolve and play a role in the small intestine, and the alkaline environment in the intestine is not conducive to the function of phytase; in addition, the dissolution time of the coating material is 30 minutes, which is longer and plays a slower role; 3. This method uses ethanol as the enteric-coated film dissolution material, which is easy to produce pollution to the product and the environment; 4. Many feed pelleting temperatures reach above 85°C, while the CN 1775067A method only involves the retention rate of 80°C

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] With 130kg cornstarch, 60kg corncob powder, 40kg marble powder, 70kg lactose, a total of 300kg of materials were mixed in a mixer for 5 minutes, then 240kg of phytase fermentation liquid was added, (this fermentation liquid was produced by the phytase strain after Submerged liquid fermentation, using glucose, ammonium sulfate and other cultures to produce liquid fermentation products, the main components are water and phytase protein, the enzyme activity is 10000U / g), and continue to mix for 10 minutes.

[0051] Put the mixed material into the hopper of the swing granulator, start the equipment, and make the material into columnar granules with a length of about 3-5mm and a diameter of about 1-3mm. Put the columnar granular material into the inner cavity of the spherical rounder, start the equipment, adjust the rotation speed to 400-600rpm, and discharge the material until the columnar particles become round particles.

[0052] Put 300kg of round granular materials into...

Embodiment 2

[0055] With 100kg cornstarch, 40kg corncob powder, 20kg marble powder, 40kg lactose, 200kg in total were mixed in mixer for 5min, then added 300kg fermented liquid (this fermented liquid is produced phytase bacterial strain through liquid submerged fermentation, utilizes Glucose, ammonium sulfate and other cultures, the liquid fermentation product produced, the main components are water and phytase protein, the enzyme activity is 8000U / g), and continue to mix for 10 minutes.

[0056] Put the mixed material into the hopper of the swing granulator, start the equipment, and make the material into columnar granules with a length of about 3-5mm and a diameter of about 1-3mm. Put the columnar granular material into the inner cavity of the spherical rounder, start the equipment, adjust the rotation speed to 400-600rpm, and discharge the material until the columnar particles become round particles.

[0057] Put 300kg of round granular materials into the drying tower, start the equipme...

Embodiment 3

[0060] Performance Testing

[0061] 1. Appearance: Sensory measurement, using a magnifying glass to check the roundness and uniformity of the particles. The coated micropill phytase prepared by the invention is microspherical, round, regular in shape and uniform in particle size.

[0062] 2. Density: sensory measurement, no broken when twisted by hand.

[0063] 3. Enzyme activity uniformity: After a batch of products are produced, 10 samples are taken at different locations, and according to GB / T18634 Feed Phytase Activity Determination-Spectrophotometric Method, combined with the relevant guidelines of the national testing agency, The phytase activity of each sample was measured and then the coefficient of variation was determined. The coated micropill prepared by the invention has high uniformity of phytase activity content, and the coefficient of variation is less than 5%.

[0064] 4. Particle size: Use a standard sample sieve to randomly test the weight of 30-60 mesh pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
viscosityaaaaaaaaaa
viscosityaaaaaaaaaa
Login to View More

Abstract

The invention relates to coated micro-pellets phytase and the preparation method thereof. The coated micro-pellets phytase of the invention comprises micro-pellets phytase and a coating layer, the grain size of the coated micro-pellets phytase is about 30 to 60 meshes, the grains are dense, and the volume weight is feasible, wherein, the micro-pellets phytase comprises carriers and phytase in an effective amount, and all carrier components in the general carrier quantity have the proportion that corn starch is 30 to 50 wt percent, corn cob meal is 10 to 30 wt percent, marble dust is 5 to 15 wtpercent, and lactose is 10 to 25 wt percent; the coating layer comprises hydroxypropyl emthylcellulose, hydroxypropyl cellulose, polyacrylic acid resin, and polyvinylpyrrolidone, and the proportion of the components is 10 to 30 wt percent:10 to 30 wt percent:30 to 50 wt percent:10 to 30 wt percent. The invention also discloses the preparation method of the coated micro-pellets phytase. The invention handpicks unique modeling palletizing and coating material, can keep the stability of the enzyme activity when the phytase products are used and stored, and increases the capacity of resisting badexternal environments of high temperature, high humidity, etc.

Description

technical field [0001] The invention relates to a coated pellet phytase and a preparation method thereof. Background technique [0002] Phosphorus is one of the mineral elements necessary for animal growth, reproduction, bone calcification and metabolism. Due to the important role of phosphorus, it is very important to provide adequate phosphorus in animal diets. Although plant-based feed materials contain a large amount of phosphorus, 60-80% of them are in the form of phytate phosphorus, but in monogastric animals and aquatic products, there is a lack of enzymes required to decompose phytate phosphorus, and the utilization rate of phosphorus is only 0. ~30%, so a large amount of inorganic phosphorus needs to be added to the feed to supplement the deficiency of phosphorus, which causes a large increase in the cost of the feed. At the same time, the phytate phosphorus in the feed cannot be used by animals, and a large amount of it is excreted outside the body of the animal, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/16C12N11/00
Inventor 李光智
Owner 北京昕大洋科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products