Technique for producing gene rHu(Cu/Zn-SOD) with one-step method

Abstract

The invention discloses an enzyme chemistry of biological engineering, in particular to a technique of applying an one-step method for preparing a gene recombinant human copper and zinc superoxide dismutase; the main technology is that a human SOD engineering bacterium is fermented and amplified, the bacterium is centrifugally collected, and then composite crushing, gradient temperature increase,the centrifugal collection of the supernatant liquid, filtration, ultra-filtration and freeze-drying are carried out, and the technique eliminates the processes of extraction, ion exchange, molecularsieve column chromatography and other separation and purification processes of the traditional preparation technique of biological engineering. The production flow is greatly shortened, each production can save the time of 30 hours, the production cost is reduced by 30 percent, and the specific activity of the human SOD product is not less than 10000u / mg (prot). The gene recombinant human SOD canbe widely applied in medicines, health care products, cosmetics, wine products, toothpastes and other SOD products.

Description

technical field [0001] The invention relates to enzyme chemistry in bioengineering, more specifically, it is a method for preparing gene recombined human source copper and zinc superoxide dismutase by one-step method. Background technique [0002] Superoxide dismutase (SOD) is a new type of enzyme preparation containing metal ions, which is widely distributed in various organisms, almost from animals to plants. It is internationally recognized as a special enzyme for scavenging oxidative free radicals (also known as free radicals). The human body will produce a large number of free radicals of aerobic elements, called superoxide free radicals, under normal metabolism or various stimuli. Excessive accumulation of superoxide free radicals in the human body can accelerate cell aging and reduce the immune function of the body, and even induce tumors. SOD can promote the decomposition of superoxide radicals, because the superoxide anion, which is a harmful substance, reacts with...

AI Technical Summary

Problems solved by technology

At present, there are generally two methods for the production of SOD. The first method is the traditional method, which is to extract SOD from animal blood. Due to the risk of exogenous infection of SOD in blood products and the antigenicity of SOD in non-human products when used in humans, in the It is difficult to play a role in medical treatment, and the cost is high, and the source of raw materials is limited. Europe has banned long-term use on the human body since 1999; the second method is to extract SOD from plants. Although it can eliminate the disadvantages of viruses and exogenous pollution, but Difficult to achieve industrial mass production due to low SOD content in plants

The above-mentioned production process of extracting SOD all adopts separation and purification processes such as extraction, ion exchange, and molecular sieve in the traditional method, and the extraction process is complicated and the yield is low.

At present, it has not been found that the application of contemporary biological science and technology to the SOD industry, the method of preparing genetically recombined human copper and zinc superoxide dismutase by one-step method, the production of SOD by genetic recombination is the most cutting-edge technology in the world, and the production of SOD by gene recombination has infinite Exogenous pollution, no immune rejection, no protein species difference, no virus residue, high purity, high activity, most suitable for human body, is a revolution in the SOD industry from technology to quality

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