Lactobacillus reuteri hollow micro-capsule and its preparing method

Abstract

The invention discloses a method for preparing a probiotic hollow microcapsule of Lactobacillus reuteri, which includes: firstly separating and purifying the Lactobacillus reuteri, then adding 5-20g of starch, 5-20g of carboxymethyl cellulose, 2-5g of sodium cellulose sulfate and 5-20g of calcium chloride into 1000ml of cultured seed extractive solution, preparing the hollow microcapsule through an extrusion method, then preparing a fermentation culture medium, fermenting and culturing for a period of time under nitrogenous atmosphere, finally applying the membrane on the capsule and drying, then obtaining the probiotic hollow microcapsule of Lactobacillus reuteri. The invention provides the method for preparing a hollow microcapsule preparation of the Lactobacillus reuteri, which prolongs the storage period of the Lactobacillus reuteri and is convenient to store, transport and use. The prepared probiotic hollow microcapsule of Lactobacillus reuteri has advantages of prolonging the survival time of probiotics at a room temperature and preventing the probiotcs from being killed by gastric acid after oral intake to allow the probiotics to enter the intestinal tract, targeted delivering and playing curative effect.

Description

technical field [0001] The invention relates to a preparation method of Lactobacillus reuteri probiotic hollow microcapsules. Background technique [0002] Lactobacillus reuteri (Latin name: Lactobacillus reuteri) is a beneficial lactic acid bacterium that inhabits the intestinal tract of humans and animals and has the ability to adhere to the intestinal epidermis. Studies have shown that the reuterin, protein, organic acid and other substances produced by the metabolism of Lactobacillus reuteri can widely inhibit the growth of gram-positive bacteria, gram-negative bacteria, yeast, fungi and pathogens, etc. Broad-spectrum antibacterial effect. Lactobacillus reuteri can be used as a feed additive to improve the distribution of intestinal flora in animals and inhibit the reproduction of harmful bacteria, thereby avoiding some intestinal diseases in animals. Lactobacillus reuteri probiotics can also be used to improve the decline in the number of beneficial bacteria in the hu...

AI Technical Summary

Problems solved by technology

[0003] Probiotic products are mainly live bacteria preparations. At present, Lactobacillus reuteri probiotics have appeared. In order to ensure the number of viable bacteria within the validity period, they are generally stored at a low temperature of 2-8°C, which brings great challenges to storage, transportation and sales. big inconvenience

Lactobacillus reuteri is an obligate anaerobic bacterium, which is easily affected by environmental factors such as oxygen, temperature, acid and alkali, so it is difficult for liquid bacterial agents to maintain its high activity for a long time

For probiotics to have a beneficial effect on the human body, they must first pass through the esophagus and stomach to reach the end of the intestinal tract and colonize on the intestinal mucosa. However, when the oral Lactobacillus reuteri liquid preparation passes through the stomach of humans and animals, The acidic environment (pH of about 2) and bile in the stomach will kill Lactobacillus reuteri and sharply reduce the number of viable bacteria, resulting in the inability of Lactobacillus reuteri to colonize in large quantities in the intestinal tract, thus affecting Lactobacillus reuteri Therapeutic and health effects of regulating intestinal flora

According to reports, the survival rate of oral freeze-dried bacterial agents in the stomach is only about one in a million, and the survival rate is extremely low.