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Artificial crystalline len with transforming growth factor resistant beta2 antibody membrane on surface and manufacturing method thereof

A transforming growth factor, intraocular lens technology, applied in the direction of prosthesis, intraocular lens, medical science, etc., can solve the problems of toxic and side effects, complex manufacturing process, and short time to maintain effective concentration, and achieve the goal of inhibiting post-onset cataract, The manufacturing method is simple and reasonable, and the effect is beneficial to the recovery of vision

Active Publication Date: 2008-09-24
SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although they can play a role in inhibiting the proliferation of residual lens epithelial cells in situ by local high-concentration drugs, and have the effect of in vitro tests, they have been found to have the following disadvantages in vivo tests: 1) coated on the surface of the intraocular lens Antiproliferative cytotoxic drugs or drug sustained release carriers, which have potential toxic side effects on corneal endothelial cells, iris, ciliary body epithelial cells, retina and other intraocular tissues; in addition, other delivery methods include intracapsular Drug lavage, intraocular implantation of biodegradable polymer drug slow-release carriers, etc. The disadvantage of the former is that the effective concentration is maintained for a short time and it is easy to affect other tissues in the eye, while the latter is easy to affect intraocular tissues or Interfere with other operations, and once toxic side effects occur, they need to be removed immediately; 2) The stability of the drug coating on the surface of the intraocular lens is relatively poor, so it is not reliable to use; 3) Their manufacturing process is relatively complicated

Method used

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  • Artificial crystalline len with transforming growth factor resistant beta2 antibody membrane on surface and manufacturing method thereof
  • Artificial crystalline len with transforming growth factor resistant beta2 antibody membrane on surface and manufacturing method thereof
  • Artificial crystalline len with transforming growth factor resistant beta2 antibody membrane on surface and manufacturing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The intraocular lens with anti-transforming growth factor β2 antibody film on the surface of this embodiment is an intraocular lens with anti-transforming growth factor β2 antibody film on the surface of the optical part and loops of the intraocular lens.

[0027] The anti-transforming growth factor β2 antibody in this example is a kind of immunoglobulin, which can inhibit post-cataract through the way of immune cell biology, because the key factor causing post-cataract is transforming growth factor β2, so adopt Anti-transforming growth factor β2 antibody can inhibit the effect of transforming growth factor β2, and inhibit extracellular matrix deposition and fibrosis with its high specificity, so it can play a role that other drugs lack in fibrosis-based post-cataract. Targeting effect, and the human antibody is not immunogenic and toxic. Therefore, after the intraocular lens with anti-transforming growth factor β2 antibody film on the surface of this embodiment is impl...

Embodiment 2

[0033] The intraocular lens used in this embodiment is a kind of in polymethyl methacrylate or soft hydrophobic polyacrylate material, and the difference from Example 1 is the method for pretreatment of the surface of the intraocular lens to obtain a negative charge, and then assemble The reagents and methods used in the process are also the same as in Example 1, that is, positively charged polylysine and negatively charged anti-transforming growth factor β2 antibody. The difference with the surface pretreatment method of Example 1 is that: after the intraocular lens is cleaned and dried according to the method of Example 1, it is immersed in a 30% sodium hydroxide aqueous solution and hydrolyzed at 80°C for 30 minutes, and its surface can produce With a large number of carboxyl ions, the surface is negatively charged under aqueous conditions.

[0034] The commonality between this embodiment and Example 1 is that the surface of the intraocular lens is negatively charged after ...

Embodiment 3

[0039] Take intraocular lens, its material can be a kind of in polymethyl methacrylate, silicone gel, soft hydrophobic polyacrylate etc., be the intraocular lens of material as silicone gel, clean and bake according to the method of embodiment 1 After drying, put it into a low-temperature plasma generator, the power of the generator is 60W, the radio frequency is 13.5kHz, the gas inside is ammonia gas, the gas pressure is maintained at 50-60Pa, and the plasma glow discharge is used for 2-5 Minutes, then take out the intraocular lens, which has amino functional groups on its surface; immerse it in a hydrochloric acid solution with a concentration of 1mol / L for 30 minutes to convert the amino hydrochloride into ammonia ions, rinse with deionized water, and blow dry with nitrogen to obtain positive charged IOL surface; then immerse the above-mentioned positively charged IOL in 0.01 mg / mL negatively charged heparin aqueous solution for 120 minutes, rinse with deionized water, and b...

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Abstract

The invention provides an artificial lentis, which contains anti-transforming growth factor beta 2 antibody membrane on the surface and can inhibit intercurrent post-cataract after cataract surgery, and also provides a the production method thereof. The production method includes the steps that: the artificial lentis is charged with positive electricity or negative electricity after the artificial lentis is cleaned, dried, and pretreated on the surface; the artificial lentis is soaked in a polyelectrolyte solution the charge of which is opposite to the surface charge of the artificial lentis for adsorbing, and rinsing the artificial lentis by deionized water, and drying the artificial lentis by nitrogen gas; the artificial lentis is soaked in a phosphate buffering solution of anti-transforming growth factor beta 2 antibody, the pH value of which is 4-10, and the carried charge of which is opposite to that of the polyelectrolyte, for adsorption; finally, the artificial lentis is rinsed by phosphate buffering solution, and the artificial lentis is dried by nitrogen gas; the alternating assembly steps are repeated. The artificial lentis of the invention can inhibit the transformation and differentiation as well as cyst membrane shrinkage of the lentis epithelial cells in a target way, and then interdicts the occurrence of the post-cataract, and has excellent biocompatibility. The production method of the invention is scientific and simple, and can ensure the activity under a dry state and the safety and reliability during medical transplantation of the anti-transforming growth factor beta 2.

Description

technical field [0001] The invention relates to an intraocular lens used for replacing the intraocular lens in cataract surgery, in particular to an intraocular lens with an anti-transforming growth factor β2 antibody film on the surface capable of inhibiting subsequent postoperative cataract and its manufacturing method . Background technique [0002] After cataract is a complication formed after extracapsular cataract extraction due to the incomplete absorption of the residual lens cortex and the remaining part of secondary opacity, so it is also called posterior capsular opacity or secondary cataract; this eye disease will directly affect to restore the patient's vision. At present, with the improvement of extracapsular cataract extraction, the incidence of post-cataract has dropped from 50% in the early stage, but 14.1-18.8% of postoperative patients still need to receive Nd-YAG laser surgery (that is, neodymium-doped yttrium aluminum Posterior capsulotomy with garnet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/34A61L27/54A61F2/16
Inventor 姚克王瑶
Owner SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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