Small-diameter tissue engineering artificial blood vessel and preparation method thereof
An artificial blood vessel and engineering technology, applied in the field of tissue engineering, can solve the problems of inappropriate selection of seed cells, limitation of blood vessel research and application, insufficient hydrophilicity, etc., and achieve broad social and economic benefits, support and maintain cells Function, reduce the effect of virus mutual infection
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[0061] (1) Preparation of decellularized umbilical vein scaffold:
[0062] Select an isolated umbilical cord that has been placed in 0-4°C heparin saline within 10 minutes and stored within 24 hours for use; then cut a 15cm-long umbilical vein from the umbilical cord; insert a glass rod with an outer diameter of 4-6mm into the umbilical cord Into the lumen of the vein; trim the adventitia of the umbilical vein so that the thickness of the adventitia is about 750 μm; store the umbilical vein in 0.25% trypsin at 4°C for 24 hours, wash it with PBS three times, and then place it in 1% In SDS solution for 24 hours; PBS solution was washed 3 times and placed in penicillin-streptomycin double antibody for 12 hours; finally, PBS was used to wash 3 times for later use; finally, an umbilical vein with a length of about 15 cm was obtained, which was milky white in appearance, soft in texture and smooth on the surface. smooth.
[0063] (2) Preparation of smooth muscle cells:
[0064] Ta...
Embodiment 2
[0079] Example 2 Biological detection of small-caliber tissue engineering artificial blood vessels——Experiment of tensile strength
[0080] The small-caliber tissue engineering artificial blood vessel prepared in Example 1 was placed on the test bench, and a part of the material was taken after a pressure of 250 mmHg for 24 hours. The tensile test showed that the burst pressure of the blood vessel was >300 mmHg.
[0081] It can be seen from the above tensile strength test that the artificial blood vessel of the present invention has very good tensile strength and is suitable for human use.
Embodiment 3
[0082] Example 3 The small-caliber tissue engineering artificial blood vessel of the present invention Zoological experiment of the present invention
[0083] Material selection and methods: Rats are male or female, weighing about 250 grams, about 1 cm of the abdominal aorta of the rat is excised, and the artificial blood vessel is placed at the resected abdominal aorta by end-to-end anastomosis. Suture continuously to complete the animal experiment.
[0084] The small-diameter tissue-engineered artificial blood vessels prepared in Example 1 and the existing GORE-TEX artificial blood vessels were taken, each with a length of 3 to 5 mm, and implanted into the abdominal aorta of different SD rats respectively. The experimental methods were as described above; The artificial blood vessel and the anastomosis between the artificial blood vessel and the coronary artery were observed at day, 2, 4, 8, 16, and 30 days. Observation results: The small-caliber tissue engineering artifici...
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