Small-diameter tissue engineering artificial blood vessel and preparation method thereof

An artificial blood vessel and engineering technology, applied in the field of tissue engineering, can solve the problems of inappropriate selection of seed cells, limitation of blood vessel research and application, insufficient hydrophilicity, etc., and achieve broad social and economic benefits, support and maintain cells Function, reduce the effect of virus mutual infection

Active Publication Date: 2012-11-21
李京倖 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They still have some problems: (1) Insufficient hydrophilicity and weak cell adhesion; (2) Can cause aseptic inflammation; (3) Insufficient mechanical strength; (4) Bioactive substances such as angiogenic factors are easy to filter (5) The price is relatively expensive, there is a certain possibility of synthesis, and the source is not easy
[0005] Non-degradable matrix materials mainly include: 1. Expanded polytetrafluoroethylene (ePTFE), which is currently used in distal limb bypass transplantation, but the source is not easy and expensive, so it is not suitable for large-scale use in China
2. Dacron material, when used, gelatin or even blood is often used to pre-coat the inner membrane of the cavity to prevent bleeding, which limits its further research and application in small-caliber tissue engineering blood vessels
2. Autologous arteries and veins, the source is limited
However, due to inappropriate selection of seed cells, complicated operation, and unsatisfactory long-term effect, no further research was carried out.

Method used

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  • Small-diameter tissue engineering artificial blood vessel and preparation method thereof
  • Small-diameter tissue engineering artificial blood vessel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0061] (1) Preparation of decellularized umbilical vein scaffold:

[0062] Select an isolated umbilical cord that has been placed in 0-4°C heparin saline within 10 minutes and stored within 24 hours for use; then cut a 15cm-long umbilical vein from the umbilical cord; insert a glass rod with an outer diameter of 4-6mm into the umbilical cord Into the lumen of the vein; trim the adventitia of the umbilical vein so that the thickness of the adventitia is about 750 μm; store the umbilical vein in 0.25% trypsin at 4°C for 24 hours, wash it with PBS three times, and then place it in 1% In SDS solution for 24 hours; PBS solution was washed 3 times and placed in penicillin-streptomycin double antibody for 12 hours; finally, PBS was used to wash 3 times for later use; finally, an umbilical vein with a length of about 15 cm was obtained, which was milky white in appearance, soft in texture and smooth on the surface. smooth.

[0063] (2) Preparation of smooth muscle cells:

[0064] Ta...

Embodiment 2

[0079] Example 2 Biological detection of small-caliber tissue engineering artificial blood vessels——Experiment of tensile strength

[0080] The small-caliber tissue engineering artificial blood vessel prepared in Example 1 was placed on the test bench, and a part of the material was taken after a pressure of 250 mmHg for 24 hours. The tensile test showed that the burst pressure of the blood vessel was >300 mmHg.

[0081] It can be seen from the above tensile strength test that the artificial blood vessel of the present invention has very good tensile strength and is suitable for human use.

Embodiment 3

[0082] Example 3 The small-caliber tissue engineering artificial blood vessel of the present invention Zoological experiment of the present invention

[0083] Material selection and methods: Rats are male or female, weighing about 250 grams, about 1 cm of the abdominal aorta of the rat is excised, and the artificial blood vessel is placed at the resected abdominal aorta by end-to-end anastomosis. Suture continuously to complete the animal experiment.

[0084] The small-diameter tissue-engineered artificial blood vessels prepared in Example 1 and the existing GORE-TEX artificial blood vessels were taken, each with a length of 3 to 5 mm, and implanted into the abdominal aorta of different SD rats respectively. The experimental methods were as described above; The artificial blood vessel and the anastomosis between the artificial blood vessel and the coronary artery were observed at day, 2, 4, 8, 16, and 30 days. Observation results: The small-caliber tissue engineering artifici...

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Abstract

The invention discloses a minorcaliber tissue engineering artificial vessel. The excised extract cell umbilical vessel is taken as a bracket, the gap part of the bracket is filled with smooth muscle cells and mechanocyte; the endothelial progenitor cells or mesenchymal stem cells are planted in the surface of the inner wall of the excised extract cell umbilical vessel. The minorcaliber tissue engineering artificial vessel has good biocompatibility and has the abilities of inducing, supporting and maintaining cell functions, thus reducing the reciprocal infection of virus among seeds. The artificial vessel produced by the invention overcomes the problem that the supply of autologous vessel plantation is limited and the failure problem of the synthesized implants for formation of thrombosisand / or endometrial hyperplasia, thus improving the long transplantation patency that the minorcaliber tissue engineering artificial vessel is planted into human body, leading the sufferers to avoid asecondary operation. The raw materials of the artificial vessel are obtained easily, the preparation method is simple, and cost is low, thus being accepted by the sufferers and having vast social benefit and economic benefit.

Description

technical field [0001] The invention relates to an artificial blood vessel, in particular to a small-diameter tissue engineering artificial blood vessel and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Coronary artery bypass grafting (abbreviation: coronary artery bypass grafting) uses autologous vein grafts in most patients (more than 95% of the total number of operations), and the patency rate of vein grafts in 15 years is about 60%. About 10% to 40% of patients after one operation need to undergo secondary or multiple bypass operations. At this time, no autologous blood vessels are available. At present, all clinical small-caliber artificial blood vessels (diameter ≤ 5mm), due to the non-physiological contact surface when anastomosing with human autologous blood vessels, the compliance of artificial blood vessels and autologous blood vessels does not match, resulting in animal experiments and clinical experiment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/50A61F2/06
Inventor 李京倖罗毅李平刘硕赵莉敏张健群尤斌孔晴宇
Owner 李京倖
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