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Polylactic acid electrospinning fibre immobilized laccase and preparation thereof

A technology of immobilizing laccase and electrospun fiber, which is applied in the field of environmental enzyme catalysis, can solve problems such as unstable properties, water body separation, complex processing, etc., to maintain activity and stability, efficient adsorption and immobilization, and less environmental conditions Effect

Inactive Publication Date: 2009-04-15
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing enzyme immobilization materials still have defects such as low immobilization efficiency, complicated processing technology, high production cost, and poor separability to varying degrees.
If inorganic materials such as silica gel and activated carbon are used as immobilized carriers, there are defects such as separation from the water body and loose links between the hydrophilic surface and the enzyme; while chitosan, polymer hydrophilic membranes, etc. unstable
In addition, the existing material preparation process is too cumbersome and the installation method is complicated, and the reactor or fixed bed must be used as the supporting part, which is limited in practical application.

Method used

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  • Polylactic acid electrospinning fibre immobilized laccase and preparation thereof
  • Polylactic acid electrospinning fibre immobilized laccase and preparation thereof

Examples

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preparation example Construction

[0024] The preparation method of the polylactic acid electrospun fiber immobilized laccase comprises two steps: the synthesis of the carrier and the immobilization of the laccase;

[0025] The synthesis steps of the carrier include:

[0026] 1) Dissolve polylactic acid (racemic) particles with a molecular weight of 100,000 in dichloromethane, add a hydrophilic block copolymer surfactant, mix evenly and stir for more than 1 hour to obtain a polylactic acid / block copolymer mixture Solution, wherein the amount of the block copolymer is 5% to 10% of the weight of the polylactic acid;

[0027] 2) The high-voltage electrospinning equipment consists of a high-voltage power supply, a spinneret and a negative plate. The mixed gel obtained in 1) was introduced into a high-voltage electrospinning device. Adjust the power supply voltage to 12-15 kV, the distance between the spinneret and the negative plate is 10 cm, and adjust the spinneret to obtain stable and continuous spraying.

[...

Embodiment 1

[0043] 1.2 g of racemic polylactic acid was dissolved in 10 g of methylene chloride to form a 10.7 g / g methylene chloride solution. Add 0.06 g of F108 block copolymer surfactant and keep stirring until completely dissolved. Introduce a high-voltage electrospinning device to be spun. Adjust the voltage of the high-voltage power supply to 14 kV, and the distance between the spinneret and the plate is 10 cm to obtain a stable and continuous jet flow. The negative plate is covered with aluminum foil to collect fibrous products. After 2 hours, when the fiber membrane reaches a certain thickness, stop spinning. The resulting fiber membrane was irradiated under an infrared lamp for 30 minutes to completely volatilize the dichloromethane. The fiber membrane was rinsed three times with deionized water to remove the residual hydrophilic block copolymer on the surface. The obtained fiber film is dried at 40 degrees for subsequent use. That is, the electrostatic spinning polylactic a...

Embodiment 2

[0046] 1.3 g of racemic polylactic acid was dissolved in 10 g of methylene chloride to form a 11.5 g / g methylene chloride solution. Add 0.1 g of F108 block copolymer surfactant and stir until completely dissolved. The voltage of the high-voltage power supply was adjusted to 12 kV, and the distance between the spinneret and the plate was 10 cm to obtain a stable and continuous jet flow. After 2 hours, when the fiber membrane reaches a certain thickness, stop spinning. Irradiate under infrared light for 30 minutes to completely volatilize dichloromethane. The fiber membrane was rinsed three times with deionized water to remove the residual hydrophilic block copolymer on the surface. The obtained fiber film is dried at 40 degrees for subsequent use. The specific surface area of ​​this sample was 43 m2 / g. The morphology of the fiber was characterized under a scanning electron microscope. The average diameter of the fiber was about 500 nanometers, and the overall shape was comp...

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Abstract

The invention relates to an immobilized enzyme catalyst-an electrospinning polylactic acid immobilized laccase used for decomposing chlorophenols pollutants in the environment and a preparation method thereof. The immobilized laccase takes a HMW (high molecular weight) polylactic acid as a vector and immobilizes the laccase on the vector through chemical bonding and cross bonding. The preparation method thereof includes two steps of the composing of the vector and the immobilizing of the laccase. The composing of the vector takes a high voltage static electrospinning technology for preparing an organic liquid containing the polylactic acid into a non-spinning fiber membrane with a fiber diameter of nanometer grade; the immobilizing of the laccase is to mix the vector with the laccase which react in a cushion liquid with the pH value equal to 4.01 for obtaining the immobilized laccase at the temperature of 4 DEG C; and then the immobilized laccase is washed and dried for obtaining the finished product of the immobilized laccase. The invention provides the novel immobilized laccase catalyst which has the advantages of good stability, high activity, convenient preparation and fast arranging.

Description

technical field [0001] The invention belongs to the technical field of environmental enzyme catalysis, in particular to a polylactic acid electrospun fiber immobilized laccase catalyst which can be used to degrade chlorophenol organic pollutants and a preparation method thereof. Background technique [0002] Laccase (Laccase, E.C.1-10-3-2) is a sugar-containing polyphenol oxidase with copper as the molecular core. Copper ion is the catalytic active center of laccase and the determinant of the catalytic activity of laccase. Laccase generally contains four copper ions, which can be divided into three types according to their spectral characteristics: one type I copper ion and one type II copper ion, two type III copper ions, and type I copper ion 2+ and type II Cu 2+ It is paramagnetic. Type I Cu 2+ Blue in color with a characteristic absorption peak at 614nm; type II Cu 2+ No characteristic absorption spectrum; Type III Cu 2 4+ For coupled ion pairs (Cu 2+ -Cu 2+ ), ...

Claims

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Application Information

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IPC IPC(8): C12N11/08C12N11/06
Inventor 牛军峰沈珍瑶殷立峰刘佳李阳
Owner BEIJING NORMAL UNIVERSITY
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