Corn and composite PCR detecting method of fumonisin toxigenic strain in corn products

A detection method and hummus technology are applied in the field of crop disease prevention and phytosanitary, which can solve the problems of not directly revealing the toxigenicity of the strain to be tested, and not completely guaranteeing the reliability of the results, saving detection time, improving accuracy, The effect of high detection accuracy
CN101418337AInactive Publication Date: 2009-04-29ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2009-04-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a composite PCR detection method for fumonisin toxicogenic strains in corn and corn products, wherein a plurality of pairs of primers are designed by application of DNAman sequence analysis software respectively on the basis of polyketide synthetase genes FUM1, serine-hexadecanoyl transferase genes FUM8 and longevity guarantee factor genes FUM17 required for biosynthesis of fumonisin, and primer matched groups with optimized specificity are obtained through experimental comparison of the primers and fusarium specific primers IstF / IstR; the fumonisin toxicogenic strains are simultaneously detected in the same reaction system; and the plurality of pairs of primers are subjected to amplification simultaneously in the same reaction system by the composite PCR technology. The composite PCR detection method comprises the following detection steps: template DNA is directly extracted from a sample to be detected by the improved SDS method; and the template DNA is subjected to composite PCR reaction, electrophoresis, dyeing and rinsing, a gel imaging device is used for observing the result and taking a picture, and finally the detection result is obtained after spectrogram analysis. The composite PCR detection method improves the detection accuracy, saves the detection time and can be massively promoted by means of a reagent kit.
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Description

technical field

[0001] The "complex PCR detection method for fumonisin-producing strains in corn and its products" of the invention belongs to the technical field of crop disease prevention and plant quarantine. Background technique

[0002] Fumonisins are a group of mycotoxins mainly produced by fungi of the genus Fusarium, such as Fusarium verticillioides (Fusarium moniliforme) and Fusarium proliferatum. It not only has acute toxicity and potential carcinogenicity to some livestock, such as causing equine encephalitis, pig pulmonary edema or liver cancer in rats, but also can cause esophageal cancer and neural tube defects in humans, seriously threatening food safety and human and animal health. healthy. Due to its wide distribution and strong toxicity, fumonisin has become another new hotspot of mycotoxin research after aflatoxin. In recent years, elevated levels of fumarin in corn have attracted the attention not only of corn growers, but also of livestock farmers, agr...

Claims

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