Method for producing 3-hydracrylic acid

A technology of hydroxypropionic acid and amino acid, applied in the field of production of 3-hydroxypropionic acid, which can solve problems such as difficult large-scale production and large-scale production

Inactive Publication Date: 2009-06-03
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because 3HP is difficult to produce on a large scale, therefore, the PHA containing 3HP component is also difficult to produce on a large scale

Method used

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  • Method for producing 3-hydracrylic acid
  • Method for producing 3-hydracrylic acid
  • Method for producing 3-hydracrylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, wild-type Pseudomonas putida KT2440 utilizes 1,3-propanediol to produce 3HP

[0024] Strain: Pseudomonas putida KT2440 (DSMZ, catalog number: DSM6125 )

[0025] The medium is composed of substances with the following concentrations: 10g / L peptone, 5g / L yeast extract, 10g / L sodium chloride, 10g / L 1,3-propanediol, and the pH value is 6.7-7.2. The solvent of the culture medium is water. Sterilize at 121°C for 20 minutes.

[0026] Culture method: Pseudomonas putida KT2440 (DSMZ, commodity catalog number: DSM6125) is connected 2 rings with inoculation loop under aseptic condition in the 500ml Erlenmeyer flask that 50ml above-mentioned culture medium is housed, at 13mm radius of rotation, rotating speed is 225rpm / min Incubate for 48 hours at 30°C in a shaker. The experiment was repeated three times, each with 10 bottles.

[0027] Processing and testing: the fermentation broth was centrifuged to remove bacteria, and 3ml of the supernatant was placed in an est...

Embodiment 2

[0036] Embodiment 2, wild-type Pseudomonas putida KT2442 utilizes 1,3-propanediol to produce 3HP

[0037] Bacteria: Pseudomonas putida KT2442 (Tsinghua University) (Franklin, F.C.H., Bagdasarian, M., Bagdasarian, M.M.and Timmis, K.N., 1981. Molecular and functional analysis of the TOL plasma pWW0 from Pseudomonas putida and cloning of genes for the entire romatire meta-cleavage pathway. Proceedings of the National Academy of Sciences USA 78, pp.7458-7462)

[0038] The medium is composed of substances with the following concentrations: 10g / L peptone, 5g / L yeast extract, 10g / L sodium chloride, 10g / L 1,3-propanediol, and the pH value is 6.7-7.2. The solvent of the culture medium is water. Sterilize at 121°C for 20 minutes.

[0039] Culture method: Put Pseudomonas putida KT2442 under sterile conditions into 2 rings with an inoculation loop in a 500ml Erlenmeyer flask containing 50ml of the above-mentioned medium, and culture it in a shaker with a rotation radius of 13mm and a ro...

Embodiment 3

[0041] Embodiment 3, wild-type Aeromonas hydrophila, ATCC7966 utilizes 1,3-propanediol to produce 3HP

[0042] Strain: Aeromonas hydrophila ATCC7966 (American Type Type Collection Center ATCC, catalog number: ATCC7966)

[0043] The medium is composed of substances with the following concentrations: 10g / L peptone, 5g / L yeast extract, 10g / L sodium chloride, 10g / L 1,3-propanediol, and the pH value is 6.7-7.2. The solvent of the culture medium is water. Sterilize at 121°C for 20 minutes.

[0044] Culture method: Put Aeromonas hydrophila, ATCC7966 (ATCC, catalog number: ATCC7966) under aseptic conditions in a 500ml Erlenmeyer flask containing 50ml of the above-mentioned medium with an inoculation loop, and rotate it at 13mm Incubate at 30° C. for 48 hours in a shaker with a radius and rotation speed of 225 rpm / min. The experiment was repeated three times, each with 10 bottles.

[0045] Processing and testing: the fermentation broth was centrifuged to remove bacteria, and 3ml of...

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Abstract

The invention discloses a method for producing 3-hydracrylic acid. The method is characterized by taking 1,3-propylene glycol as a raw material, and producing the 3-hydracrylic acid by microbes which express 1,3-propylene glycol dehydrogenase and aldehyde oxidase. The microbes which express the 1,3-propylene glycol dehydrogenase and the aldehyde oxidase are microbes which naturally express the 1,3-propylene glycol dehydrogenase and the aldehyde oxidase, or recombinant microbes which can express the 1,3-propylene glycol dehydrogenase and the aldehyde oxidase and are obtained by transferring coding gene of 1,3-propylene glycol dehydrogenase and coding genes of the aldehyde oxidase into a microbe host. By the method, the 1,3-propylene glycol is taken as the raw material, content of the 3-hydracrylic acid per liter of a culture medium is up to 0.24-12.1g.

Description

technical field [0001] The present invention relates to a method for producing 3-hydroxypropionic acid. Background technique [0002] 3-Hydroxypropionic acid (3HP) is a viscous liquid with a logarithmic acid dissociation constant (pKA) of 4.5 in water, easily soluble in water and ethanol, and miscible with ether (Merck Index, 11 th Edition, 4681). 3HP has a wide range of applications, and can be used as a precursor for chemical synthesis and drug synthesis to produce coatings, adhesives, water treatment chemicals, and personal skin care products. Currently reported chemical products that can be synthesized from 3HP include Ethyl ethoxy propionate (EEP), acrylamide compounds, acrylates, acrylic acid polymers, and 1,3-malonic acid. 3HP can also be used as a precursor for the synthesis of ultra-high-performance polyester plastic PHP and a high-performance monomer component precursor for the synthesis of high-performance biodegradable plastic polyhydroxyalkanoate (PHA) (MattC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12N1/20C12N1/21C12N15/53C12N15/70C12N15/74C12R1/40C12R1/19C12R1/15C12R1/01
Inventor 陈国强石振宇张磊吴琼
Owner TSINGHUA UNIV
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