Starch synthase gene promoter with seed-specific expression and application thereof

Abstract

In the invention, a Genomic walking method is employed to design a specific nested primer according to a cDNA sequence (GenBank Accession No.: AF036891) of a maize dull 1 gene, maize genome is taken as a template to separate a maize starch synthase gene dull 1 promoter from maize. The promoter has a spatiotemporal specific expression property, can drive target genes to efficiently express in plant seeds at grain filling stage, but is not expressed at leaves, leaf veins and roots, and has wide use in research of plant bioreactor. The promoter lays a foundation for researching an expression regulating and control mechanism of plant starch anabolism, creates a condition for starch biosynthesis regulation and control in gene engineering technology, and plays an important role in the research of plant starch improvement and the plant bioreactor.

Description

Technical field

[0001] The present invention relates to the fields of plant molecular biology and plant genetic engineering, in particular to a seed-specifically expressed starch synthase gene promoter and its application. Background technique

[0002] Seeds are the main source of food for humans and animals, as well as raw materials for many industries, and are closely related to human production and life. However, due to the limitations of plants themselves, the protein and other ingredients contained in seeds cannot fully meet human needs. With the rapid development of modern molecular biology, the application of genetically modified plants in theoretical research and plant breeding has become more and more extensive. Through DNA recombination technology, plants can not only obtain some insect resistance, disease resistance, and stress resistance that they do not possess. , It can also synthesize some essential amino acids that it does not have in the seeds to improve the nutr...

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