Preparation method of immobilized lipase used for grease hydrolysis
A technology of immobilized lipase and oil hydrolysis, which is applied in the direction of fixing on/in organic carriers, fermentation, etc., can solve the problems of unbearable actual production, increase of environmental control costs, and environmental harm, and achieve low-cost sources, Low cost, environment-friendly effect
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Embodiment 1
[0023] 1) Dissolve cellulose acetate in a dimethylacetamide / acetone mixture with a volume ratio of 3:2, stir and dissolve at room temperature to form a homogeneous solution with a concentration of 130 g / L, and let stand to remove air bubbles. obtain spinning solution;
[0024] 2) carry out electrospinning with the spinning solution that makes in step 1), spinning flow rate 1mL / h, spinning voltage is 17kV, and the distance from nozzle to receiving plate is 15cm, makes cellulose acetate superfine fiber film, SEM photo see figure 1 ;
[0025] 3) Place the cellulose acetate microfiber membrane in an ethanol solution of potassium hydroxide with a concentration of 0.5M, soak for 3 hours, take out the membrane after the reaction is completed, rinse it with deionized water several times, and dry it for use;
[0026] 4) Soak the cellulose acetate microfiber membrane obtained in step 3) with absolute ethanol, then immerse in epichlorohydrin, take it out after reacting at 37°C for 3 ho...
Embodiment 2
[0030] The steps are the same as in Example 1, but in step 1), cellulose acetate butyrate is used instead of cellulose acetate, and the enzyme loading is 17 mg enzyme / g membrane. Electron micrographs of cellulose acetate butyrate microfiber membranes are shown in figure 2 .
[0031] The prepared cellulose acetate butyrate microfiber membrane immobilized lipase was used for soybean oil hydrolysis, the activity was 267U / g immobilized enzyme; for castor oil hydrolysis, the activity was 256U / g immobilized enzyme; For the hydrolysis of seed oil, the activity is 268U / g immobilized enzyme; for the hydrolysis of peanut oil, the activity is 264U / g immobilized enzyme; for the hydrolysis of cottonseed oil, the activity is 237U / g immobilized enzyme; for the hydrolysis of rice bran oil, the activity is 248U / g immobilized enzyme; for algae oil hydrolysis, the activity is 249U / g immobilized enzyme; for the mixed oil hydrolysis of soybean oil and castor oil with a volume ratio of 1:1, the a...
Embodiment 3
[0033]1) Dissolve cellulose acetate butyrate in a dimethylacetamide / acetone mixture with a volume ratio of 1:1, stir and dissolve at room temperature to form a uniform solution with a concentration of 20g / L, and let it stand to remove air bubbles to obtain spinning solution;
[0034] 2) Electrospinning is carried out with the spinning solution prepared in step 1), the spinning flow rate is 5mL / h, the spinning voltage is 10kV, and the distance from the nozzle to the receiving plate is 10cm, and cellulose acetate butyrate superfine fibers are obtained membrane;
[0035] 3) Place the cellulose acetate butyrate ultrafine fiber membrane in an ethanol solution of potassium hydroxide with a concentration of 1M, soak for 1 hour, take out the membrane after the reaction is completed, rinse it several times with deionized water, and dry it for use;
[0036] 4) Soak the cellulose acetate butyrate superfine fiber membrane obtained in step 3) with absolute ethanol, then immerse in epichlo...
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