Method for deeply processing deer placenta

A technology for deep processing and deer tires, applied in the fields of food science and bioengineering, can solve the problems of resource waste, environmental pollution, waste of beneficial components of deer tires, etc., and achieve the effects of reducing processing costs, avoiding environmental pollution, and enhancing immunity.

Inactive Publication Date: 2010-05-12
铁岭鹿宝堂药业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, during the processing and production of the original deer tires, the residues produced are directly discarded, which not...

Method used

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  • Method for deeply processing deer placenta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the preparation of active deer placenta dry powder

[0015] 1) Take fresh deer placenta to remove tendons and membranes, and cut it into pieces; according to the weight ratio, deer placenta:normal saline is 1:1.5-2.5, take the material, grind it in a grinder, and fully homogenize it; completely freeze it at -40~-20°C, Take it out and thaw it completely at room temperature, freeze and thaw repeatedly 3 to 5 times, centrifuge at 5000 rpm for 10 minutes, the supernatant is the placenta extract, and the precipitate is for later use; wherein, the physiological saline is 0.9% sodium chloride solution.

[0016] 2) Take the placenta extract, add acetone according to the volume ratio of placenta extract and acetone at 3:1, mix well, centrifuge at 8000rpm for 10min, and take the supernatant. According to the volume ratio of the collected supernatant and acetone is 3:1, add acetone, mix well, centrifuge at 8000rpm for 20min, collect the supernatant and distill under ...

Embodiment 2

[0023] Embodiment 2, the preparation of pure product of deer fetus immunomodulatory factor

[0024] 1) Get the active deer placenta extract obtained in Example 1, through ultrafiltration (ultrafiltration template 10000NMWL, ultrafiltration pressure: 0.5kg / cm 2 ) and dialysis (molecular weight cut-off 8000dal), the active ingredients with a molecular weight of 3000-8000dal were collected, desalted by gel filtration chromatography (Sephadex G25), and sterilized (0.22 μm membrane) to obtain the extract of deer fetal immune regulatory factor.

[0025] 2) The obtained deer fetal immunomodulatory factor extract was further freeze-dried to obtain a pure deer fetal immune regulatory factor.

[0026] The yield of deer fetus immunoregulatory factor is: 3.5g of pure deer fetus immune regulator factor can be obtained per kilogram of deer fetus.

[0027] Test results:

[0028] The antitumor activity and lymphocyte proliferation activity of deer fetal immunomodulatory factor were determin...

Embodiment 3

[0032] The preparation of embodiment 3 compound amino acids

[0033] 1) with the step 1) of embodiment 1.

[0034]2) Weigh the precipitate, add 6 liters of 3mol / L sulfuric acid per kilogram of the precipitate, hydrolyze for 30 minutes at a microwave power of 500W, filter to obtain the filtrate, adjust the filtrate to pH6.0 with NaOH, and obtain the compound amino acid. After preparing the deer fetus extract solution per kilogram of deer fetus, about 0.5-0.6kg of leftovers can be produced. Microwave-assisted acid hydrolysis can produce 730 mg of compound amino acids per gram of waste precipitation, and the content of each amino acid is shown in Table 1.

[0035] The sulfuric acid used can also be replaced by hydrochloric acid or nitric acid.

[0036] Test results:

[0037] The protein content in compound amino acids was determined by Folin-phenol method, the degree of hydrolysis was determined by formaldehyde titration and Kjeldahl method, and the amino acid content of compo...

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Abstract

The invention relates to a method for deeply processing deer placenta. The method comprises the technical scheme of selecting deer placenta and normal saline in a weight ratio of 1:1.5-2.5, grinding and homogenizing the mixture; refrigerating the mixture at the temperature of between 40 and 20 DEG C below zero, melting the mixture at room temperature, performing refrigeration and melting repeatedly, and centrifuging the mixture to collect supernatant, wherein the supernatant is placenta extract and precipitate is for later use; adding acetone into the placenta extract, evenly mixing, and centrifuging the mixture to collect the supernatant; adding acetone into the supernatant in the same ratio, evenly mixing and centrifuging the mixture, distilling the supernatant under reduced pressure, and sterilizing and ultrafiltering the supernatant to prepare active deer placenta extract; selecting the active deer placenta extract to collect component comprising molecular weight of between 3,000 and 8,000 dal through ultrafiltration and dialysis, desalinizing the supernatant through gel-filtration chromatography, and sterilizing the supernatant through membranes to prepare deer placenta immunoregulatory factor extract; and preparing compound amino acid by using microwave to assist acid hydrolysis of precipitate. The method can extract more bioactivator from the deer placenta, not only ensures that waste protein resources can be utilized sufficiently, but also can reduce the treatment cost for the protein, and avoids causing environmental pollution at the same time.

Description

technical field [0001] The invention belongs to the fields of food science and bioengineering, in particular to a method for deep processing deer placenta by using bioengineering technology to obtain new products with biological activity. Background technique [0002] Placenta is also known as "Placenta hominid". Wide range of clinical applications, often used for fatigue and weakness, bone steaming and night sweats, cough and asthma due to deficiency of both lungs and kidneys, impotence and seminal emission due to deficiency of kidney qi, decline of essence and blood, infertility, infertility, and oliguria. As the link between the mother and the fetus during pregnancy, it has important physiological functions, can secrete a variety of hormones, enzymes, contains a variety of proteins, antibodies, phospholipids, growth factors, cytokines and small proteins that can stimulate the antibody immune system Molecular active substances. Placenta has the effect of enhancing resist...

Claims

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Application Information

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IPC IPC(8): A23L1/312A23L1/313A23L1/30A23K1/10A23K10/20A23L13/20A23L13/30
Inventor 胡风庆边媛媛
Owner 铁岭鹿宝堂药业集团有限公司
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