Method for producing ethanol by fermenting straws

An ethanol and straw technology, applied in the biological field, can solve the problems of accumulation, coenzyme redox imbalance, affecting ethanol yield, etc., and achieve the effects of increasing ethanol yield, shortening fermentation period, and improving utilization efficiency

Active Publication Date: 2010-05-12
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, these two coenzymes cannot be directly converted in Saccharomyces cerevisiae, and their regeneration processes are independent of each other, resulting in an intracellular coenzyme redox imbalance; on the other hand, under the oxygen-limited conditions of ethanol fermentation, NADH accumulation in yeast cells , cannot be reduced in time to the coenzyme NAD required by xylitol dehydrogenase + , so that the intermediate product xylitol accumulates, thereby affecting the yield of ethanol

Method used

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  • Method for producing ethanol by fermenting straws
  • Method for producing ethanol by fermenting straws
  • Method for producing ethanol by fermenting straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 The isomerization test of xylose

[0040] Use commercially available xylose isomerase to carry out xylose conversion xylulose test, the total reaction volume is 10L, under 120r / min stirring, the reaction system is: D-xylose 30% (W / V), CoCl 2 ·6H 2 O 2×10 -4 mol / L, MgSO 4 ·7H 2 O 2×10 -3 mol / L, the amount of adding xylose isomerase is 10 6 U / L. React at pH 5.0 and 35°C, and take the reaction solution after 12 hours for processing. The analysis was carried out by high performance liquid chromatography.

[0041] 1. Preparation of standard solution: take 500 mg each of xylose standard product and xylulose standard product respectively, and use double distilled water (ddH 2 O) dissolve and make up to 50ml.

[0042] 2. Treatment of the isomerization reaction solution: Centrifuge the reaction solution at 12,000 rpm for 20 minutes, take the supernatant for ultrasonic degassing, and filter it with a 0.45 μm organic phase filter.

[0043] 3. The chromatographi...

Embodiment 2

[0046] Example 2 Synchronous saccharification of corn stalks and xylose isomerization fermentation to produce ethanol after dilute acid pretreatment

[0047] 1. Dilute acid pretreatment of corn stalks

[0048] Take 10kg of dry corn stalks, add water at a solid-to-liquid ratio of 1:6, and then add sulfuric acid at a ratio of 1:2, heat to 120°C for 2 hours, centrifuge, and wash twice with warm water at 60°C. Take solid phase analysis: moisture is 63.46%, except moisture, cellulose in dry material is 58.64%, hemicellulose 4.37%, lignin 25.21%, ash 4.29%.

[0049] 2. Lignocellulose saccharification and xylose isomerization fermentation to produce ethanol after pretreatment

[0050] Put 1.5kg of the above-mentioned pretreated lignocellulose (dry material) into a 12L fermenter, add 4.5L of distilled water, heat to 115°C for 20min, and then add enzyme preparation when the temperature drops to 50°C, every 1g of the above Add 20U cellulase, 110U xylanase and 100U xylose isomerase to th...

Embodiment 3

[0051] Example 3 Synchronous saccharification of corn stalks and xylose isomerization fermentation to produce ethanol after alkali pretreatment

[0052] 1. Alkaline pretreatment of corn stalks

[0053] Take 10 kg of dried corn stalks, add water at a solid-to-liquid ratio of 1:6, and then add 1.5% NaOH by weight, heat to 100°C for 1 hour, centrifuge, and wash twice with warm water at 60°C. Take the solid phase analysis: water content 68.46%, except water, cellulose 62.3%, hemicellulose 23.6%, lignin 7.2%, ash content 4.7% in the dry material.

[0054] 2. Lignocellulose saccharification and xylose isomerization fermentation to produce ethanol after pretreatment

[0055] Put 1.5kg of the above-mentioned pretreated lignocellulose (dry material) into a 12L fermenter, add 4.5L of distilled water, heat to 115°C and keep it for 20min, when the temperature drops to 50°C, add enzyme preparation, every 1g of the above dry 30U cellulase, 300U xylanase and 280U xylose isomerase were adde...

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Abstract

The invention provides a method for producing ethanol by synchronizing saccharification, isomerization and fermentation of lignocellulose. In the method, cellulase, xylanase and xylose isomerase are used to enzymolyze the lignocellulose, and enzymolysis products are fermented to obtain the ethanol. The method adopts the synchronous saccharification, isomerization and fermentation of the lignocelluloses to produce the ethanol, and has the advantages of reducing the negative feedback inhibition of the enzymolysis products on the cellulase and the xylanase, increasing enzymolysis rate, shortening a fermentation period, reducing production cost and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to enzymatically hydrolyzing lignocellulose into glucose and xylulose by using cellulase, xylanase and xylose isomerase, and yeast uses glucose and xylulose as carbon sources to ferment A method of producing ethanol. Background technique [0002] In the process of studying cellulose fermentation to produce ethanol, in order to prevent sugar accumulation and products from inhibiting enzymatic hydrolysis, and improve the catalytic hydrolysis efficiency of cellulase, the Simultaneous Saccharification and Fermentation (SSF) mode was proposed, which has received extensive attention. Pay attention to. In the process of simultaneous saccharification and fermentation, the monosaccharides produced by enzymatic hydrolysis are digested by yeast in time, and the concentration of monosaccharides in the system is kept at a low level, which can achieve the purpose of eliminating the inhibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 李荣杰薛培俭徐斌段绪果薛亮胡长浩
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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