Method for synthesizing betulic acid by carrying out biocatalysis on betulin

Abstract

The invention discloses a method for synthesizing betulic acid by carrying out biocatalysis on betulin, comprising the following steps: (1) preparing armillaria luteo-uirens ZJUQH wet cells; (2) adding armillaria luteo-uirens ZJUQH wet cells into ionic liquid or a two-phase system for pre-culture to obtain a pre-culture system; (3) adding betulin solution in the pre-culture system for fermentation culture for 6-24 hours at 25-30 DEG C to obtain transformed culture solution; (4) processing the transformed culture solution to obtain betulic acid. The two-phase system is mixed solvent of ionic liquid and normal hexane, or mixed solvent of ionic liquid and phosphoric acid buffer solution. Compared with a conventional method, the inventive method has higher product yield, shorter catalytic reaction time, simple product separation, lower volatility of ionic liquid system, better biocompatibility and no pollution to the environment.

Description

technical field

[0001] The invention relates to the fields of bioengineering and microbial fermentation, in particular to a method for biocatalyzing betulin to synthesize betulinic acid. Background technique

[0002] Biocatalysis is the process of using enzymes or microbial cells to catalyze certain chemical reactions. It has the advantages of strong specificity, high catalytic efficiency, environmental friendliness, and mild operating conditions. Compared with biocatalytic reactions in aqueous phase, non-aqueous phase biocatalysis has its unique advantages. However, traditional organic solvents usually limit the activity and selectivity of enzymes, and cause a lot of industrial pollution.

[0003] Ionic liquids (ILs), as a new type of green solvent, are salts composed of organic cations and inorganic anions or organic anions, which are liquid at room temperature or lower temperature (<100°C), have negligible vapor pressure, It has the characteristics of stability, high...

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