Unlock instant, AI-driven research and patent intelligence for your innovation.

Variable-volume microfluidic chip for colorimetric assay

A microfluidic chip, colorimetric technology, applied in color/spectral property measurement, measurement device, material analysis by observing the effect on chemical indicators, etc. The operation steps are lengthy and cumbersome, etc.

Inactive Publication Date: 2011-12-07
FUDAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] It can be seen from the above examples that the traditional colorimetric method is a pure chemical detection method, which takes a long time and has cumbersome procedures. , or a calibration curve needs to be drawn, or there are many interference factors, or more expensive instruments are required, and the professional skills of the operators are high, which can only be done in the laboratory, which cannot meet the requirements of online measurement, such as field testing, sudden On-site monitoring of sudden pollution accidents, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Variable-volume microfluidic chip for colorimetric assay
  • Variable-volume microfluidic chip for colorimetric assay
  • Variable-volume microfluidic chip for colorimetric assay

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0039] Example 1: Determination of dissolved oxygen in water by iodometric method (Winkler method)

[0040] 1. Traditional iodometric method

[0041] The principle of the traditional iodometric method is to add manganese sulfate and alkaline potassium iodide to the water sample to form manganese hydroxide precipitate. At this time, the nature of manganese hydroxide is extremely unstable, and it rapidly combines with dissolved oxygen in water to form manganese oxide. Adding concentrated sulfuric acid makes the combined dissolved oxygen (as MnMnO 3 Exist in the form of) react with the potassium iodide added in the solution to precipitate iodine I 2 . Because solid iodine I 2 The solubility in water is very small (0.0013mol / L), but I 2 It is easily soluble in KI solution, when I 2 In KI solution with I 3 - form exists. Quantitatively released I 2 , with starch as indicator, sodium thiosulfate Na 2 S 2 o 3 Titrate the standard solution until the blue color disappears ...

example 2

[0055] Example 2: Detection of total phosphorus in water

[0056] 1. Phosphomolybdenum blue method

[0057] Inject the sample into the reaction tank 3, then inject the ammonium molybdate-sulfuric acid solution, mix well, inject the mixed solution of potassium antimony tartrate-sodium sulfate-ascorbic acid, and measure the absorbance with a 700nm yellow LED after mixing well.

[0058] 2. Colorimetric determination of yellow complexes in molybdate-vanadate solution

[0059] Inject the sample into the reaction cell (3), then inject the molybdic acid-ammonium vanadate solution, mix well, and measure the absorbance with a blue LED with a wavelength of 420nm.

[0060] Phosphorus absorbance value = sample absorbance value - digested blank solution absorbance value

[0061] Note: Environmental water samples need to be tested after high temperature and high pressure or ultraviolet digestion.

example 3

[0062] Example 3: Detection of total nitrogen in water

[0063] 1. National standard method (alkaline potassium persulfate-ultraviolet spectrophotometry)

[0064] After the water sample undergoes potassium sulfate-high temperature and high pressure thermal oxidation or ultraviolet light oxidation, titanium dioxide photocatalytic oxidation or ultrasonic-ozone oxidation, inject sodium hydroxide solution or sulfuric acid solution to adjust the pH to 5-9, then inject it into reaction pool 3, and then inject potassium persulfate Solution, mix well, inject hydrochloric acid after cooling, measure absorbance with ultraviolet light with wavelength of 220nm and 275nm respectively after mixing.

[0065] From A=A 220 -2A 275 Calculate the absorbance of the sample and subtract the absorbance of the blank to obtain the corrected absorbance, and use the calibration curve to calculate the corresponding total nitrogen content.

[0066] Note:

[0067] (1) Dissolved organic matter has a st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of environment and water quality monitoring, in particular to a variable-volume microfluidic chip for colorimetric assay. In the invention, automatic operation can realized on fussy liquid adding procedures by the variable-volume microfluidic chip and a pump valve arranged the periphery of the variable-volume microfluidic chip, and the color changes ofa reactant or an indicator is detected by a photoelectric detector so as to improve the detection sensitivity and detection limit, the needed sample quantity is few, the detection time is short, and the online measurement can be realized in real time. The invention can be used to detect dissolved oxygen, biochemical oxygen demand, total phosphorus, total nitrogen, ozone, chlorine, organics, heavymetal element and other indexes, and is suitable for sudden pollution incident emergency monitoring and field site test.

Description

technical field [0001] The invention belongs to the technical field of environmental detection, and specifically relates to a microfluidic chip for measuring various components in water by using the principle of colorimetry, and is mainly used in multiple fields such as environmental monitoring, water quality monitoring, scientific research, and teaching. Background technique [0002] Colorimetry is a widely used traditional chemical analysis method, which can measure dissolved oxygen (DO) and biochemical oxygen demand (BOD) in water. 5 20 ), ozone, residual chlorine, chlorine dioxide (ClO 2 ) and organic and inorganic reducing substances in water (such as S 2- , SO 3 2- , S 2 o 3 2- , Sn 2+ etc.), the determination of total phosphorus and total nitrogen. [0003] The principle of determination of dissolved oxygen in water is to use the redox characteristics of iodine to perform colorimetric determination through the yellow color of iodine itself or adding starch as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/01G01N21/75G01N21/79
Inventor 鲍军波隋国栋
Owner FUDAN UNIV