Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for material with high density fixed biologically functional molecule

A technology of functional molecules and biologically active molecules, which is applied in the fields of tissue engineering, polymer materials, and biological detection, can solve the problems of low density of immobilized biomolecules and complicated reaction process, and achieve the promotion of cell adhesion and spreading, simple operation, and applicability wide effect

Inactive Publication Date: 2010-08-25
WUHAN UNIV OF TECH
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: aiming at the problems of complex reaction process and low density of immobilized biomolecules existing in the current bioactive surface preparation method, to provide a method that is easy to operate, can immobilize biomolecules at high density and repel non-specific proteins at the same time. Surface Modification Methods by Adsorption

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for material with high density fixed biologically functional molecule
  • Preparation method for material with high density fixed biologically functional molecule
  • Preparation method for material with high density fixed biologically functional molecule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 2.2 mL of methacryloyl chloride in 10 mL of chloroform was added dropwise to a solution of 2.3 g of NHS and 3.3 mL of triethylamine in 20 mL of chloroform at 0°C. After the dropwise addition was completed, the reaction was carried out at room temperature for 4 hours. The reaction solution was washed with saturated ice brine, and the organic phase was dried with anhydrous magnesium sulfate. The precipitate was filtered, the solvent was concentrated, and then recrystallized in a mixed solution of 0.8 mL ethyl acetate and 6 mL n-hexane to obtain NHSMA as a monomer.

[0036] Under a nitrogen atmosphere, 7.93 g of OEGMA, 312 mg of 2,2-bipyridine and 143 mg of cuprous bromide were dissolved in 15 mL of a mixed solution of methanol and water (4:1). The above solution was ultrasonically transferred to a silicon chip with α-bromobutyryl bromide initiator fixed on the surface for 5 minutes, reacted at room temperature for 2 hours, then stopped the reaction with copper bromide / bi...

Embodiment 2

[0039] 2.2 mL of methacryloyl chloride in 10 mL of chloroform was added dropwise to a solution of 2.3 g of NHS and 3.3 mL of triethylamine in 20 mL of chloroform at 0°C. After the dropwise addition was completed, the reaction was carried out at room temperature for 4 hours. The reaction solution was washed with saturated ice brine, and the organic phase was dried with anhydrous magnesium sulfate. The precipitate was filtered, the solvent was concentrated, and then recrystallized in a mixed solution of 0.8 mL ethyl acetate and 6 mL n-hexane to obtain NHSMA as a monomer.

[0040] Under a nitrogen atmosphere, 7.93 g of OEGMA, 312 mg of 2,2-bipyridine and 143 mg of cuprous bromide were dissolved in a mixed solution of 15 mL of methanol and water (1:1). After the above solution was ultrasonicated for 5 minutes, it was transferred to a silicon wafer with α-bromobutyryl bromide initiator fixed on the surface, reacted at room temperature for 2 hours, and the surface was cleaned with ...

Embodiment 3

[0043] 2.2 mL of methacryloyl chloride in 10 mL of chloroform was added dropwise to a solution of 2.3 g of NHS and 3.3 mL of triethylamine in 20 mL of chloroform at 0°C. After the dropwise addition was completed, the reaction was carried out at room temperature for 4 hours. The reaction solution was washed with saturated ice brine, and the organic phase was dried with anhydrous magnesium sulfate. The precipitate was filtered, the solvent was concentrated, and then recrystallized in a mixed solution of 0.8 mL ethyl acetate and 6 mL n-hexane to obtain NHSMA as a monomer.

[0044] Under a nitrogen atmosphere, 7.93 g of OEGMA, 312 mg of 2,2-bipyridine and 143 mg of cuprous bromide were dissolved in 15 mL of a mixed solution of methanol and water (2:1). The above solution was ultrasonicated for 5 minutes and then transferred to a silicon wafer with α-bromobutyryl bromide initiator fixed on the surface. After reacting for 2 hours at room temperature, the surface was cleaned with a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method for material with bioactivity functional surface, which comprises the steps of: grafting polymethylacrylic acid oligomerization gylcol ester (POEGMA) with excellent rejecting non-specific protein adsorption capacity on the surface of the material by an atom transfer radical polymerization (ATRP) technique; then grafting polymethylpentene acryloyl oxylsuccinimide at the tail end of the POEGMA by using a secondary initiation of ATRP; and fixing biological molecules containing amino in a high-density way by using a larger number of active ester group contained in the side chain of PNHSMA. The bioactivity functional surface provided by the method can obviously increase the grafting density of the surfaces of the biological molecules, simultaneously reduces the undesirable influence caused by non-specific protein adsorption, effectively keeps the activity of the fixed biological molecules and can fix various biological molecules and is suitable for more extensive biomedical fields.

Description

technical field [0001] The invention relates to the fields of biological detection, tissue engineering and polymer materials, in particular to a method for preparing a biological functional surface capable of high-density immobilization of biological molecules while repelling non-specific protein adsorption. Background technique [0002] Using biological principles to immobilize biologically active substances such as proteins, cell growth factors, enzymes, and polypeptides on the surface of the material can effectively improve various biological functions of the material (such as anticoagulant performance, activity of binding to antibodies, and ability to bind to cells) Adhesion, etc.), which has important application value in various biomedical fields such as biological detection, tissue engineering, protein separation and purification, and artificial implant materials (Pascal J.et al.Angew.Chem.Int.Ed., 2008 , 47: 9618-9647; Chen H. et al. Prog. Polym. Sci., 2008, 33: 1059...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L27/54A61L27/16A61L33/06G01N1/28G01N33/545C08F293/00C03C17/28C04B41/48C07K1/14C07K17/08
Inventor 陈红张燕霞于谦
Owner WUHAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com