Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Effective phenylamine degrading strain as well as use and use method thereof

An aniline-degrading bacteria and high-efficiency technology, applied in chemical instruments and methods, biochemical equipment and methods, microorganism-based methods, etc., can solve the problems of high toxicity of aniline, difficult degradation, slow degradation rate, etc. Ease of operation and robust effect

Inactive Publication Date: 2010-09-15
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of frequent aniline pollution accidents, high toxicity of aniline, difficult degradation, and slow degradation rate, and provide a high-efficiency degrading bacterium capable of rapidly degrading high-concentration aniline and its application in dealing with sudden pollution accidents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective phenylamine degrading strain as well as use and use method thereof
  • Effective phenylamine degrading strain as well as use and use method thereof
  • Effective phenylamine degrading strain as well as use and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Screening and isolation of aniline-degrading bacteria Rhodoccocus sp.AN-P1 and treatment of aniline wastewater

[0012] Collect activated sludge from the aerobic pool of Chengdu No. 1 Sewage Treatment Plant. The sludge samples were enriched and cultured at 10% inoculum volume at 30°C and 160r / min, gradually increasing the concentration of aniline. Then streak and separate on MSB agar plates containing 1000mg / L aniline, select colonies with different morphological characteristics, retransfer to MSB medium with 1000mg / L aniline, culture under the same conditions to verify its aniline degradation ability, and further purify Until a pure culture strain with degradation ability is obtained.

[0013] Synthetic Medium (MSB): Contains Na per 1L 2 HPO 4 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, trace element solution 5mL, aniline 1g. Add 15g / L agar powder to the solid medium.

[0014] A highly efficient aniline-degrading bacterium AN-P1 was finally isolated and...

Embodiment 2

[0018] Example 2: AN-P1 Simulated Emergency Treatment of Aniline Sudden Pollution Accident Waste Liquid

[0019] A certain amount (500mg / L, 1000mg / L, 2000mg / L) of aniline is added to domestic sewage to simulate the impact of aniline entering the drainage pipe network on the sewage biological treatment system after a sudden pollution accident. The simulated experimental system is divided into two groups, one group is the control system, that is, the conventional activated sludge treatment system (MLSS=2000±100mg / L); the second group is the bio-augmented emergency treatment with the inoculation amount of 0.3‰AN-P1 strain System (MLSS=2000±100 mg / L). 30°C, natural pH, 160rpm shaker cultivation simulation treatment (dissolved oxygen controlled at 3-5mg / L). The 500mg / L group was sampled every 1h to determine the degradation effect of aniline and COD; the 1000mg / L and 2000mg / L groups were sampled every 4h to determine the degradation effect of aniline and COD. The treatment results...

Embodiment 3

[0029] Example 3: Emergency treatment of waste liquid from aniline pollution accident based on SBR process

[0030] Take domestic sewage plant sludge and sewage to build an SBR process system. The reactor numbers are No. 1 (conventional SBR system) and No. 2 (emergency SBR system), with an effective volume of 4.5L, MLSS=2000±100mg / L, and a steam-water ratio of 32: 1 (dissolved oxygen 3~5mg / L), room temperature, HRT during normal operation is 8h, after 7 days of stable operation, add 2000mg / L aniline to No. 1 and No. 2 reactors, and inoculate AN-P1 bacteria into No. , Sampling and determination of aniline content and COD every four hours. The measurement results are shown in Table 2. After the emergency treatment, the normal operation of the SBR process was resumed to treat domestic sewage, and the effluent quality indicators of COD and ammonia nitrogen were tested. The test results are shown in Table 3.

[0031] As shown in Table 4, the test results of aniline and COD show t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of environment protection, in particular an effective phenylamine degrading strain as well as use and use method thereof. In the invention, a code number of the effective phenylamine degrading bacteria is AN-P1; and the effective phenylamine degrading bacteria is authenticated to be rhodococcus sp, which is preserved in China committee for culture preservation of general microorganism centre with the preservation number of CGMCC No.2783 in DEC.4th, 2008. The effective phenylamine degrading strain is applied to high-concentration phenylamine waste water treatment or phenylamine pollution incident emergency treatment. The effective phenylamine degrading strain has the characteristics of strong endurance capability of high-concentration phenylamine, high degradation speed, no secondary pollution, safe use, no bad influence on microorganism in an original processing system and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a high-efficiency degrading bacterium capable of rapidly degrading aniline and its application in biological treatment of high-concentration aniline wastewater and emergency treatment of aniline sudden pollution accidents. Background technique [0002] Aniline is the main harmful pollutant in the wastewater discharged from oil refinery, dyestuff, pesticide, medicine and other industries, and is the most common water pollutant. Aniline is a poisonous and harmful "three-cause" substance that endangers human health. At present, physicochemical and biological methods are mainly used for the treatment of aniline wastewater, but there are problems such as low treatment efficiency, high cost, serious secondary pollution, and complicated operation in the treatment of aniline by physicochemical methods; as the most widely used wastewater treatment process, bio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C02F3/34C02F1/58C02F3/12C12R1/01C02F101/38
CPCY02W10/10
Inventor 李旭东武洪杰谭周亮
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com