Method for extracting cobamamide

Abstract

The invention relates to a method for preparing cobamamide. The method comprises the following steps of: a, taking vitamin B 12 fermentation solution, and hydrolyzing and filtering under the light-resistant condition; b, adsorbing filter liquor by using a macroporous resin column, and washing and desorbing to obtain primary desorption solution; c, evaporating and concentrating the primary desorption solution to obtain concentrated solution; d, adding a flocculating agent into the concentrated solution for filtering to obtain purification solution; e, adsorbing the purification solution by using a chromatographic resin column, and developing and desorbing to obtain secondary desorption solution; f, adsorbing the secondary desorption solution by using an aluminum oxide column, and developing and desorbing to obtain crystallizing stock solution; and g, adding acetone into the crystallizing stock solution with stirring for crystallizing, and filtering and drying to obtain a finished product. The impurity content of a product obtained by the method is below 1.0 percent, the cobamamide content is over 98.0 percent, the yield of the product is over 50 percent, and the total yield of vitamin B 12 is over 80 percent. The method has the characteristics of simplicity, convenience, easy operation, high extraction yield, high purity of the product and low production cost.

Description

technical field [0001] The invention relates to an extraction method of adenosylcobalamin, in particular to an improved extraction method of adenosylcobalamin. Background technique [0002] Adenosylcobalamin is a B vitamin 12 a type of coenzyme B 12 , it is a complex complex composed of cobalt ion as the center and four pyrrole rings, benzimidazole and 5-deoxyriboadenosine, molecular formula: C 72 h 100 CoN 18 o 17 P, molecular weight: 1579.57, its chemical structure is as follows: [0003] [0004] Adenosylcobalamin is dark red crystal or crystalline powder, odorless and tasteless, easy to absorb moisture in the air, and easy to decompose when exposed to light. Adenosylcobalamin belongs to vitamin drugs and is mainly used for the treatment of pernicious anemia and other megaloblastic anemia. It can also be used for the treatment of various malnutrition and hemorrhagic anemia, neuritis, neuralgia and neurological disorders. It can also treat leukopenia caused by ra...

AI Technical Summary

Problems solved by technology

It is especially distressing to the technicians that the crystallization process cannot be stirred, otherwise there will be turbidity, which will result in a low crystallization yield.

Although the product quality of the prior art can meet the quality requirements of the Chinese Pharmacopoeia, the quality is still general, and the yield of adenosylcobalamin in this process is only 30%, the total yield of the system is only 60%, and the alumina regeneration steps are cumbersome. The operation intensity is high, the amount of waste produced is large, and the investment in environmental protection is high, resulting in high production costs

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