Method for extracting cobamamide

A technology of adenosylcobalamin and an extraction method, which is applied in the field of improved adenosylcobalamin extraction, can solve the problems of large waste production, turbidity, inability to start stirring, etc., and achieves the effects of increasing batch processing volume and increasing yield

Active Publication Date: 2011-01-19
HEBEI HUARONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is especially distressing to the technicians that the crystallization process cannot be stirred, otherwise there will be turbidity, which will result in a low crystallization yield.
Although the product quality of the prior art can meet the quality requirements of the Chinese Pharmacopoeia, the quality is still general, and the yield of adenosylcobalamin in this process is only 30%, the total yield of the system is only 60%, and the alumina regeneration steps are cumbersome. The operation intensity is high, the amount of waste produced is large, and the investment in environmental protection is high, resulting in high production costs

Method used

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  • Method for extracting cobamamide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] will contain vitamin B 12 20Kg of fermented liquid is hydrolyzed and filtered to obtain the filtrate, and then the filtrate is adsorbed on about 10m 3 In the macroporous resin, wash and desorb to obtain a solution, and the solution is concentrated to obtain a concentrate; add divalent zinc salt or trivalent aluminum salt, vitamin B 12 : flocculant=20: 1 (molar ratio), adjust pH to 7.5 with 30% sodium hydroxide, after filtering, then suspend filter cake with water and filter again, repeat this operation several times, combine all filtrate vitamin B 12 The purified solution; the purified solution is adsorbed on 3m 3 In the chromatographic resin, use a low proportion of acetone water after washing with water, and the weight ratio of acetone and water can be selected to be 15:100. The solution is developed to obtain other types of vitamin B containing 5.0Kg 12 developing solution, and other types of vitamin B 12 After the ribbon of color is completely removed, then use ...

Embodiment 2

[0031] will contain vitamin B 12 20Kg of fermented liquid is hydrolyzed and filtered to obtain the filtrate, and then the filtrate is adsorbed on about 10m 3 In the macroporous resin, wash and desorb to obtain a solution, and the solution is concentrated to obtain a concentrate; add divalent zinc salt or trivalent aluminum salt, vitamin B 12 : flocculant=10: 1 (molar ratio), adjust pH to 7.2 with 30% sodium hydroxide, after filtering, then water suspension filter cake filters again, repeats this operation several times, merges all filtrate vitamin B 12 The purified solution; the purified solution is adsorbed on 3m 3 In the chromatographic resin, use low proportion of acetone water after washing with water, that is, the weight ratio of acetone and water is 5: 100 solution to develop the layer, and obtain other types of vitamin B containing 4Kg 12 developing solution, and other types of vitamin B 12 After the ribbon of color is completely removed, then use a high proportion o...

Embodiment 3

[0033] will contain vitamin B 12 20Kg of fermented liquid is hydrolyzed and filtered to obtain the filtrate, and then the filtrate is adsorbed on about 10m 3 In the macroporous resin, wash and desorb to obtain a solution, and the solution is concentrated to obtain a concentrate; add divalent zinc salt or trivalent aluminum salt, vitamin B 12 : flocculant=15: 1 (molar ratio), adjust pH to 7.4 with 30% sodium hydroxide, after filtering, then water suspension filter cake filters again, repeats this operation several times, merges all filtrate vitamin B12 The purified solution; the purified solution is adsorbed on 3m 3 In the chromatographic resin, use the acetone aqueous solution of low proportion after washing with water, the weight ratio of acetone and water is 10: 100, carry out development layer, obtain the other type vitamin B that contains 3.8Kg 12 developing solution, and other types of vitamin B 12 After the color band of color band is completely removed, then use the a...

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Abstract

The invention relates to a method for preparing cobamamide. The method comprises the following steps of: a, taking vitamin B 12 fermentation solution, and hydrolyzing and filtering under the light-resistant condition; b, adsorbing filter liquor by using a macroporous resin column, and washing and desorbing to obtain primary desorption solution; c, evaporating and concentrating the primary desorption solution to obtain concentrated solution; d, adding a flocculating agent into the concentrated solution for filtering to obtain purification solution; e, adsorbing the purification solution by using a chromatographic resin column, and developing and desorbing to obtain secondary desorption solution; f, adsorbing the secondary desorption solution by using an aluminum oxide column, and developing and desorbing to obtain crystallizing stock solution; and g, adding acetone into the crystallizing stock solution with stirring for crystallizing, and filtering and drying to obtain a finished product. The impurity content of a product obtained by the method is below 1.0 percent, the cobamamide content is over 98.0 percent, the yield of the product is over 50 percent, and the total yield of vitamin B 12 is over 80 percent. The method has the characteristics of simplicity, convenience, easy operation, high extraction yield, high purity of the product and low production cost.

Description

technical field [0001] The invention relates to an extraction method of adenosylcobalamin, in particular to an improved extraction method of adenosylcobalamin. Background technique [0002] Adenosylcobalamin is a B vitamin 12 a type of coenzyme B 12 , it is a complex complex composed of cobalt ion as the center and four pyrrole rings, benzimidazole and 5-deoxyriboadenosine, molecular formula: C 72 h 100 CoN 18 o 17 P, molecular weight: 1579.57, its chemical structure is as follows: [0003] [0004] Adenosylcobalamin is dark red crystal or crystalline powder, odorless and tasteless, easy to absorb moisture in the air, and easy to decompose when exposed to light. Adenosylcobalamin belongs to vitamin drugs and is mainly used for the treatment of pernicious anemia and other megaloblastic anemia. It can also be used for the treatment of various malnutrition and hemorrhagic anemia, neuritis, neuralgia and neurological disorders. It can also treat leukopenia caused by ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/167C07H1/08
Inventor 陈学军李振东任静任杰王欢牛静霞左志勇赵强赵浪涛李树民谭坤王旭
Owner HEBEI HUARONG PHARMA
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