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Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof

A technology of cellulomonas and bacterial strains, applied in fiber treatment, plant fiber, biochemical fiber treatment, etc., can solve the problems of unrealized industrialization, no report on the application of hemp fiber colloid decomposition, etc., and shorten the degumming cycle , Improve the combing output rate, and the effect of not being easily polluted

Inactive Publication Date: 2011-04-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of microorganisms and the enzymes they produce in flax degumming can not only improve the yield and quality of fibers, consume less energy, be safe to use, and reduce environmental pollution, it has a good prospect, but its industrialization has not been realized.
Although cellulomonas strains and their hydrolytic enzymes have been used for taxane conversion (CN200710012698.9), lignocellulose decomposition (CN200910085651.4) and plant cellulolytic agents (CN200610071920.8), they have not been found in flax The Application Report in Decomposition of Colloids of Etc. Hemp Fiber

Method used

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  • Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof
  • Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof
  • Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Screening and Obtaining of Flax Degumming Strains

[0058] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0059] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0060] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of col...

Embodiment 2

[0079]In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g of potassium dihydrogen phosphate, 0.0177g of dipotassium hydrogen phosphate, 0.0588g of sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature ...

Embodiment 3

[0089] The flax degumming process is as follows:

[0090] Put 5g of the original flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, the liquid volume is 100ml, degumming in a shaker at 40°C and 200rpm for 1 day , 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the linen, and stop degumming.

[0091] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached more than 90 percent.

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Abstract

The invention relates to a cellulomonas fimi DA 8 bacterial strain as well as an acquiring method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has flax degumming activity. The cellulomonas fimi bacterial strain screened by the method has the advantages of large enzyme yield, complete enzyme system, no toxicity, rough culturing condition and the like; after used for flax degumming, the composite degumming enzyme solution not only greatly shortens a degumming period, but also reduces the cost, the fiber splitting degree is improved, and the degumming efficiency reaches 95%. The degumming method requires basically the same equipment required in the traditional warm water degumming method so that the method is especially suitable for improving a process on the basis of the traditional equipment in old enterprises; the cellulomonas fimi bacterial strain screened with the method not only can be used for flax raw-stem degumming, roving scouring, flax fabric biological pretreatment, but also can be used for bast fiber degumming and biological pretreatment of bast fiber plants, such as ramie, jute, hemp and the like.

Description

Technical field [0001] The invention belongs to the field of Cellulomonas strains and their applications, and particularly relates to a Cellulomonas DA8 strain and its obtaining method and application. Background technique [0002] Linen textiles are favored by consumers due to their "green and health-care" characteristics and are one of the most popular textile products in the international market. There are more than 20 countries and regions in the world where fiber flax is grown. In 2004, my country's fiber flax planting area reached 160,000 acres, ranking first in the world. Heilongjiang Province is my country's main fiber flax production base, with planting area and output accounting for more than 70% of the country's total. However, my country's annual flax fiber output is only 40,000-50,000 tons, with a gap of more than 50%. The deficiencies in flax degumming technology result in poor fiber quality, low flax length and carding rate, weak strength, and poor spinnabi...

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04D06M16/00C12S11/00C12R1/01D06M101/06
Inventor 董政娥丁若垚郁崇文张兴群
Owner DONGHUA UNIV
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