Method for fractionation of soybean 7S globulins and 11S globulins of low phytic acid

A technology for fractionation and soybean protein, applied in the field of fractions rich in 11S globulin, which can solve the problems of solubility change, reduction of phytic acid enrichment, impact on component purity, etc., to improve fractionation efficiency, increase nutritional value, Effect of avoiding the desalination step

Inactive Publication Date: 2011-11-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can significantly reduce the enrichment of phytic acid in the two fractions. When no precipitant is used, the solubility of the soybean protein component that has undergone enzymatic hydrolysis to reduce phytic acid is significantly changed, which is conducive to the further purification of the 11S insoluble fraction. However, since the precipitation of the 11S fraction is carried out abov

Method used

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  • Method for fractionation of soybean 7S globulins and 11S globulins of low phytic acid
  • Method for fractionation of soybean 7S globulins and 11S globulins of low phytic acid
  • Method for fractionation of soybean 7S globulins and 11S globulins of low phytic acid

Examples

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Embodiment 1

[0028] The defatted soybean meal (NSI=91, protein weight content 49%) was crushed and passed through a 60-mesh sieve to obtain defatted soybean powder, mixed with water at a mass ratio of 1:15, extracted at room temperature and pH 8.0 for 1 hour, and then centrifuged The defatted soybean protein extract is obtained. The obtained defatted soybean protein extract was adjusted to pH 5.8 with hydrochloric acid and heated to 40°C. Add phytase ("PHYTASE", produced by BASF, Germany) to the solution (phytic acid content is 1.80% by weight of protein) in an amount of 800 μ per gram of protein, and perform enzyme treatment for 2 hours. Adjust the pH to 6.8 with 2mol / L hydrochloric acid, and add calcium chloride, the concentration of calcium chloride in the solution is 25mM. After stirring at room temperature for 10 min, centrifuge with a batch centrifuge (3000 g) to separate the soluble part containing 7S globulin and the insoluble part containing 11S globulin, and the feed solution is...

Embodiment 2

[0041] The defatted soybean meal (NSI=91, protein content 49%) was crushed and passed through a 60-mesh sieve to obtain defatted soybean powder, mixed with water at a mass ratio of 1:15, extracted at room temperature and pH 8.0 for 1 hour, and then centrifuged to obtain Defatted Soy Protein Extract. The resulting defatted soybean milk was adjusted to pH 6.8 with hydrochloric acid and heated to 50°C. Add German BASF "PHYTASE" phytase to this solution (phytic acid content is 1.80% protein weight), the addition amount is 600 μ of phytase per gram of protein, and carry out enzyme treatment for 2 hours. Adjust the pH to 6.4 with 2mol / L hydrochloric acid, add 5mM calcium chloride, stir at room temperature for 10min, and use a decanter to separate the soluble part containing 7S globulin and the insoluble part containing 11S globulin. part. During centrifugation, the feed solution was kept at room temperature. The electrophoretic patterns of the soluble and insoluble fractions afte...

Embodiment 3

[0050] The defatted soybean meal (NSI=91, protein content 49%) was crushed and passed through a 60-mesh sieve to obtain defatted soybean powder, mixed with water at a mass ratio of 1:15, extracted at room temperature and pH 8.0 for 1 hour, and then centrifuged to obtain Defatted Soy Protein Extract. The resulting defatted soybean milk was adjusted to pH 3 with hydrochloric acid and heated to 40°C. Add German BASF "PHYTASE" phytase (German BASF "PHYTASE") to this solution (phytic acid content: 1.80% protein weight), the addition amount is 1000 μ per gram of protein, and carry out enzyme treatment for 1 hour. Adjust the pH to 5.8 with 2mol / L hydrochloric acid, add 5mM calcium chloride, stir at room temperature for 10min, and centrifuge with a batch centrifuge (3000g) to separate the soluble fraction containing 7S globulin and 11S globulin the insoluble part. Compared with Comparative Example 3, the purity of the 11S component and the 7S component is higher. Correspondingly, t...

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Abstract

The invention discloses a method for fractionation of soybean 7S globulins and 11S globulins of low phytic acid, which comprises the following steps of: dissolving degreased soybean meal in 10-20 times weight of water, controlling pH value, extracting and separating at the room temperature to remove bean dregs and then obtain a soybean protein extracting solution; adjusting the pH value of the soybean protein extracting solution to 3.0-6.8 in weak acid, adding phytase, carrying out enzyme treatment for 5 minutes to 3 hours at the temperature of 20-70 DEG C to obtain an enzymolysis protein solution; adding a calcium or magnesium ion precipitator in the enzymolysis protein solution, controlling the concentration of calcium or magnesium ions in the enzymolysis protein solution to 5-25mM, adjusting pH to 5.8-6.8, and carrying out centrifugal separation on fractions containing the 7S globulins and fractions containing the 11S globulins. The method disclosed by the invention has the advantages of realizing the accurate separation of the insoluble 11S globulins and the soluble 7S globulins in the higher pH value condition and obtaining two components capable of obviously reducing the content of phytic acid.

Description

technical field [0001] The invention relates to a solution containing soybean protein, which is processed by decomposing phytic acid and fractionated by using a precipitant to obtain a fraction rich in 7S globulin and a fraction rich in 11S globulin low in phytic acid. Background technique [0002] According to the sedimentation constant during ultracentrifugation analysis, soybean storage protein can be further divided into 2S, 7S, 11S and 15S globulins. 7S globulin and 11S globulin have different unique properties, such as viscosity, cohesion, surface activity, etc. Therefore, by fractionating 7S globulin and 11S globulin, it is possible to utilize the different properties of the protein components, and it is expected to expand the industrial application of the protein. [0003] Phytic acid, on the other hand, is an organophosphate chemical (phytate: 6 phosphate groups attached to inositol) that is found mainly in the form of calcium, magnesium and potassium salts in plan...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K14/415
Inventor 杨晓泉腾紫刘翀齐军茹
Owner SOUTH CHINA UNIV OF TECH
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