Pectinase immobilizing carrier, and preparation method and application thereof
A technology of immobilized carrier and pectinase, which is applied in the field of enzyme engineering, can solve the problems of no carrier, etc., and achieve the effects of preventing environmental pollution, good biocompatibility, and beneficial to industrial production
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Embodiment 1
[0036] Stir and disperse with deionized water and soluble starch to form starch milk with a concentration of 30%. Weigh FeCl at a ratio of 1:1.5 3 , FeCl 2 , dissolved in deionized water to 10 times the starch milk, poured into the starch milk, and placed in a 65°C water bath to raise the temperature. After reaching the temperature, 0.5M NaOH solution was added dropwise to adjust the pH value to 10, and ultrasonic treatment was performed at 25KHz and 900W power for 30min. Place the treated liquid in a 65°C water bath and stir for 1.5 hours. After the reaction, let it stand and cool to room temperature, neutralize it with glacial acetic acid, wash it three times with 95% ethanol, and use a magnet with a residual magnetization of 12.3T The magnetic starch microspheres are separated from the liquid, the supernatant is poured off, and vacuum-dried at -45° C. and 20 Pa to obtain nanoscale pectinase magnetic starch microsphere carriers. The carrier is detected by a laser diffract...
Embodiment 2
[0038] Stir and disperse with deionized water and cornstarch to form starch milk with a concentration of 40%. Weigh Fe at a ratio of 1:2 2 (SO 4 ) 3 , FeSO 4 , dissolved in deionized water to 15 times the starch milk, poured into the starch milk, and placed in a 65°C water bath to raise the temperature. After reaching the temperature, 0.5M KOH solution was added dropwise to adjust the pH value to 11, and ultrasonic treatment was performed at 20KHz and 450W power for 20min. Place the treated liquid in a 65°C water bath and stir for 2 hours. After the reaction, let it stand and cool to room temperature, neutralize it with dilute hydrochloric acid solution to neutrality, wash it with 95% ethanol three times, and use a magnet with a residual magnetization of 12.3T The magnetic starch microspheres are separated from the liquid, the supernatant is poured off, and vacuum-dried at -45° C. and 20 Pa to obtain nanoscale pectinase magnetic starch microsphere carriers. The particle s...
Embodiment 3
[0040] Stir and disperse with deionized water and cornstarch to form starch milk with a concentration of 50%. Weigh FePO at a ratio of 1:1 4 , Fe 3 (PO 4 ) 2 , dissolved in deionized water to 13 times the starch milk, poured into the starch milk, and placed in a 65°C water bath to raise the temperature. After reaching the temperature, 0.5M sodium acetate solution was added dropwise to adjust the pH value to 9, and ultrasonic treatment was performed at 20KHz and 650W power for 25min. The treated liquid was placed in a 65°C water bath and stirred for 2.5 hours. After the reaction was completed, it was left to cool to room temperature, neutralized to neutrality with dilute sulfuric acid solution, washed 3 times with 95% ethanol, and the residual magnetization was 12.3T. The magnet separates the magnetic starch microspheres from the liquid, pours off the supernatant, and vacuum-dries at -45°C and 20Pa to obtain the nano-scale pectinase magnetic starch microsphere carrier. The...
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