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Three-dimensional network-like chitosan-calcium carbonate nano composite material as well as preparation method and cell compatibility thereof

A composite material, chitosan technology, applied in the application field of the composite material in cell culture, can solve the problem of low mechanical properties of the composite material, and achieve the effect of good material cell-interface, good mechanical strength, and uniform distribution

Inactive Publication Date: 2012-02-08
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of the composite material prepared by the above method are not high, and its use as a cell scaffold material has certain limitations.

Method used

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  • Three-dimensional network-like chitosan-calcium carbonate nano composite material as well as preparation method and cell compatibility thereof
  • Three-dimensional network-like chitosan-calcium carbonate nano composite material as well as preparation method and cell compatibility thereof
  • Three-dimensional network-like chitosan-calcium carbonate nano composite material as well as preparation method and cell compatibility thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of chitosan-calcium carbonate fiber network composites

[0041] According to chitosan and calcium carbonate mass ratio is 5~5.5: 1 batching, the present embodiment takes the upper, middle and lower three points of this ratio range as representative experiment, and its chitosan and calcium carbonate mass ratio are 5.00: 1 , 5.25:1, 5.50:1, the preparation steps are as follows:

[0042] 1) Dissolve 0.8190g, 0.8600g, and 0.9010g chitosan in 200ml deionized water, adjust the pH to 5.0 with 2M HCl solution, stir overnight with magnetic force, and then adjust the pH to 5.0;

[0043] 2), take 50ml of the above-mentioned three different concentrations of solutions, first add 0.0660g of calcium chloride dihydrate, the final concentration is 9mM, stir magnetically, then add 0.0711g of ammonium bicarbonate, the final concentration is 18mM, continue to stir, and then Adjust the pH of the solution to 5.5;

[0044] 3), open the CHI-660 workstation online, use the above ...

Embodiment 2

[0051] The chitosan-calcium carbonate composite material cytocompatibility experiment prepared in embodiment 1

[0052] (Take chitosan and the composite material that calcium carbonate mass ratio is 5.25: 1 for experiment)

[0053] The preparation steps are as follows:

[0054] 1), the bare stainless steel sheet and the chitosan-calcium carbonate composite material prepared as a base with the stainless steel sheet are cut into a square with a side length of 0.8 cm.

[0055] 2) Sterilize both sides of the cut sample with alcohol and ultraviolet light, then place it in a 24-well culture plate, digest the overgrown cells in the culture bottle, and dilute to a density of 1×10 4 / ml pieces. The cells were seeded in a 24-well culture plate, and 200ul of cells and 800ul of medium were added to each well. At 37°C, 5% CO 2 Cultivate in an incubator (3 control wells are set for each group of samples when measuring fluorescence, and 6 control wells are set for each group of samples w...

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Abstract

The invention discloses a three-dimensional network-like chitosan-calcium carbonate nano composite material as well as a preparation method and cell compatibility thereof. In the preparation method, the chitosan-calcium carbonate nano composite material is prepared by taking chitosan as a carbon source, calcium chloride dehydrate, ammonium bicarbonate and secondary distilled water as raw materials on the surface of stainless steel by virtue of a one-step coelectrodeposition method. The biocompatibility of the chitosan-calcium carbonate network-like nano composite structure is studied by virtue of in-vitro cell culture. The electrodeposition method is simple and controllable; and the chitosan-calcium carbonate fibrous network-like structure is firmly combined with a substrate, and has strong mechanical performance and excellent biocompatibility. A good experimental result is achieved by using the chitosan-calcium carbonate fibrous network-like structure as a cytoskeleton material. The preparation method is simple in process, low in cost and environmentally-friendly; and the three-dimensional network-like chitosan-calcium carbonate nano composite material is in accordance with the standard of a biomaterial of a cell culture medium in tissue engineering, and can be applied to bone repair.

Description

technical field [0001] The invention relates to a preparation method of a three-dimensional network chitosan and calcium carbonate nanocomposite material, in particular to a preparation method of constructing a chitosan-calcium carbonate fiber network composite nanomaterial on a stainless steel sheet as a working electrode, and the Applications of composite materials in cell culture. Background technique [0002] Bone defects such as severe trauma, tumor resection, bone infection, and congenital defects are very common. The resulting bone damage and bone loss are major health problems that greatly affect a person's ability to survive and quality of life. Therefore, developing effective bone regeneration therapies is an important long-term goal in the clinical study of mineralized tissues. The repair of bone tissue, spinal fusion, and artificial prosthesis replacement have a huge demand for bone graft materials. Currently bone reconstruction surgical treatment options are a...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/34A61L27/04A61L27/20C12M3/00C25D15/00
Inventor 龚静鸣张伟
Owner HUAZHONG NORMAL UNIV
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