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Tissue culture medium for anoectochilus roxburghii

A medium and tissue culture technology, applied in the field of medium for clematis tissue culture, can solve the problems of not forming a standardized breeding and artificial cultivation system, not forming a large-scale production, and low survival rate of artificial cultivation, etc. Inexpensive, simple production method, and the effect of multiplying reproduction

Inactive Publication Date: 2012-06-20
HANGZHOU DINGYUN AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the wild clematis is now on the verge of extinction, many experts and scholars have carried out tissue culture rapid propagation and artificial cultivation techniques of clematis through the efforts of researchers; however, due to the low survival rate of artificial cultivation, the current overall The scale of production is small and scattered, the breeding has not been commercialized, no standardized breeding and artificial cultivation system has been formed, and its production has not formed a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 (mother seedling culture medium):

[0025] Select strong mother seedlings (take every 1-2 nodes of the stems of Anologia clematis), the composition and content of the medium are (weight per liter): NAA 1mg / L, 6BA 1mg / L, sugar 25g / L , Huabao No. 1 1g / L, Huabao No. 5 1g / L, peptone 2g / L, agar powder 5g / L, banana or potato 70g / L, activated carbon (80 mesh) 1g / L, including macronutrients and trace elements , iron salts and MS of organic components, the rest is water.

[0026] The macroelements are: potassium nitrate 700mg / L, ammonium nitrate 700mg / L, potassium dihydrogen phosphate 70mg / L, magnesium sulfate 170mg / L, calcium chloride 450mg / L;

[0027] Trace elements are: potassium iodide 0.4mg / L, boric acid 3mg / L, manganese sulfate 10mg / L, zinc sulfate 4mg / L, sodium molybdate 0.05mg / L, copper sulfate 0.005mg / L, cobalt chloride 0.005mg / L;

[0028] Iron salt: disodium edetate 17mg / L, ferrous sulfate 12mg / L;

[0029] The organic ingredients are: inositol 50mg / L, g...

Embodiment 2

[0033] Embodiment 2 (mother seedling culture medium):

[0034] Select strong mother seedlings (take 1-2 nodes of the stems of Anolus clematis), the composition and content of the medium are (weight per liter): NAA1mg / L, 6BA2mg / L, sugar 25g / L, flowers Bao No. 1 1.5g / L, Huabao No. 5 1.5g / L, peptone 2.5g / L, agar powder 5.5g / L, banana or potato 70g / L, activated carbon (150 mesh) 1g / L, the rest is water;

[0035] The MS includes the following contents of macroelements, trace elements, iron salts and organic components:

[0036] The macroelements are: potassium nitrate 1200mg / L, ammonium nitrate 1000mg / L, potassium dihydrogen phosphate 100mg / L, magnesium sulfate 220mg / L, calcium chloride 300mg / L;

[0037] Trace elements are: potassium iodide 0.6mg / L, boric acid 4mg / L, manganese sulfate 15mg / L, zinc sulfate 6mg / L, sodium molybdate 0.2mg / L, copper sulfate 0.02mg / L, cobalt chloride 0.2mg / L;

[0038] Iron salt: disodium edetate 25mg / L, ferrous sulfate 18mg / L;

[0039] The organic ing...

Embodiment 3

[0043] Embodiment 3 (strong seedling culture medium):

[0044] 1) Take 1-2 nodes of the terminal bud of the mother seedling, the composition and content of the medium are (weight per liter):

[0045] NAA2mg / L, 6BA4mg / L, sugar 25g / L, Huabao No. 1 2g / L, Huabao No. 5 2g / L, peptone 3.5g / L, agar powder 6g / L, banana or potato 85g / L, activated carbon ( 200 mesh) 2g / L, including MS of macroelements, trace elements, iron salts and organic components, and the rest is water.

[0046] The macroelement is: potassium nitrate 1600mg / L, ammonium nitrate 1500mg / L, potassium dihydrogen phosphate 150mg / L, magnesium sulfate 320mg / L, calcium chloride 400mg / L;

[0047]Trace elements are: potassium iodide 0.8mg / L, boric acid 5mg / L, manganese sulfate 20mg / L, zinc sulfate 8mg / L, sodium molybdate 0.4mg / L, copper sulfate 0.03mg / L, cobalt chloride 0.03mg / L;

[0048] Iron salt: disodium edetate 40mg / L, ferrous sulfate 30mg / L;

[0049] The organic ingredients are: inositol 50mg / L, glycine 1.5mg / L, thiam...

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Abstract

The invention relates to a tissue culture medium for anoectochilus roxburghii to solve the technical problems that the culture medium needs to be capable of quickening growth of anoectochilus roxburghii in tissue culture, reducing infection rate and increasing breeding multiple, and a method for making the culture medium needs to be simple, convenient and low in cost. The technical scheme includes that the tissue culture medium for anoectochilus roxburghii comprises, in terms of composition and content, banana or potato 50-100g / L, NAA (1-Naphthaleneacetic acid) 0.1-3mg / L, 6-BA (6-benzylaminopurine) 0.1-5mg / L, white sugar 25g / L, Hyponex No.1 1-3g / L, Hyponex No.5 1-3g / L, peptone 2-4g / L, agar powder 5-7g / L, activated carbon 0.1-3g / L, a MS (Murashige and Skoog medium) which comprises major elements, trace elements, ferric salt and organic ingredients, and the balance of water with a pH value 5.8-6.0.

Description

technical field [0001] The invention relates to a plant culture medium, in particular to a culture medium for tissue culture of Aurantia clematis. Background technique [0002] Anoectochilus, Latin name Anoectochilus Formosanus Hayata, is a plant of the Orchidaceae genus Anoectochilus. It has the functions of ornamental leaves and flowers. It is a treasure of indoor flowers with high ornamental value, but its main value lies in medicinal purposes. Clematis is known as "Golden Grass", "Miraculous Medicine", "King of Medicine", "Bird Ginseng" and other laudatory titles. According to the determination of relevant departments, it is found that the amino acid composition, composition, content and anti-aging active trace element content of Aurantia clematis are higher than those of wild American ginseng. The whole herb is used for medicinal purposes. It is sweet, light, flat, and cool in nature and flavor. It has the functions of clearing heat, cooling blood, dispelling wind and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王莹王雁
Owner HANGZHOU DINGYUN AGRI DEV
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