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Production process for compounded culture medium

A production process and culture medium technology, applied in the direction of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of unclear composition and unstable quality of the medium, and achieve the benefits of three wastes treatment, reasonable composition ratio, and production The effect of horizontal stabilization

Inactive Publication Date: 2013-03-06
QINGDAO CREAT MEDIUM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of unclear composition and unstable quality of traditional medium, and to provide a production process of compound medium by adopting protein compounding technology and homogenization deployment technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Production of cottonseed protein

[0030] The cottonseed is peeled into 2-4 petals by toothed stick sheller, and then the kernel shell is separated. The shelled material is separated from the cotton kernel by a three-layer stepped separation sieve. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. The separated cotton kernels are softened in a drum softening pot, and the water content is adjusted. The softening temperature is 80°C. Drying machine drying, drying temperature 85 ℃, after drying, the cotton kernel moisture is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to remove phenols, and dry the materials after dephenolization to produce detoxified cottonseed protein;

[0031] 2) Production of peanut protein / soybean protein

[0032] The peanut / soybean is cleaned, dried, cooled, peeled, flaked, and then leached with a mixed solvent of ethanol. In the mixed solvent, th...

Embodiment 2

[0038] 1) Production of cottonseed protein

[0039] The cottonseed is peeled into 2-4 petals by toothed stick sheller, and then the kernel shell is separated. The shelled material is separated from the cotton kernel by a three-layer stepped separation sieve. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. The separated cotton kernels are softened in a drum softening pot, and the water content is adjusted. The softening temperature is 60°C. Drying machine drying, drying temperature 85 ℃, after drying, the cotton kernel moisture is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to remove phenols, and dry the materials after dephenolization to produce detoxified cottonseed protein;

[0040] 2) Production of peanut protein / soybean protein

[0041] The peanut / soybean is cleaned, dried, cooled, peeled, flaked, and then leached with a mixed solvent of ethanol. In the mixed solvent, th...

Embodiment 3

[0047] 1) Production of cottonseed protein

[0048] The cottonseed is peeled into 2-4 petals by toothed stick sheller, and then the kernel shell is separated. The shelled material is separated from the cotton kernel by a three-layer stepped separation sieve. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. The separated cotton kernels are softened in a drum softening pot, and the moisture is adjusted. The softening temperature is 70°C. Drying machine drying, drying temperature 85 ℃, after drying, the cotton kernel moisture is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to remove phenols, and dry the materials after dephenolization to produce detoxified cottonseed protein;

[0049] 2) Production of peanut protein / soybean protein

[0050] The peanut / soybean is cleaned, dried, cooled, peeled, flaked, and then leached with a mixed solvent of ethanol. In the mixed solvent, the mix...

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Abstract

The invention relates to a production process for compounded culture medium finely and deeply processed with agricultural products such as cottonseed, soybean, peanut and the like as raw materials, wherein the compounded culture medium is suitable for microbial growth and metabolism. The invention provides a following technical scheme that: the production process for the compounded culture medium comprises the steps of producing cottonseed protein, producing peanut protein / soybean protein, compounding for the first time and compounding for the second time. The production process for the compounded culture medium disclosed by the invention has the following beneficial effects that: 1) the culture medium of the invention meets the microbial requirement in the fermentation process and can be effectively utilized, the redundant part produced in the fermentation culture can be prevented from entering the fermentation waste liquid and the clean production can be realized; 2) with the culture medium of the invention, the problems of low fermentation titer, unstable production and high pollution level of fermentation waste liquid caused by too many kinds of nitrogen sources added and great quality fluctuation in the fermentation production process are solved; and 3) the culture medium of the invention comply with the target microbial growth and metabolism characteristics and has the advantages of good microbial utilization effect, low product cost, improved production quality and stable production level.

Description

technical field [0001] The invention relates to the fine deep processing of cottonseed, soybean, peanut and other agricultural products as raw materials to produce a compound culture medium suitable for the growth and metabolism of microorganisms. Background technique [0002] The total annual production of antibiotics in my country is about 50,000 tons. The production of these antibiotic raw materials is produced by industrial fermentation, and the total demand for nitrogen source medium is more than 100,000 tons. The output of my country's three major fermentation products is about 1 million tons, enzyme preparations such as amylase, protease, cellulase, glucoamylase, etc., amino acids such as lysine, L-alanine, glutamic acid and aspartic acid Fermentation production requires a large amount of culture medium. The annual output of biopesticides such as Jinggangmycin, Bacillus thuringiensis, and Abamectin is hundreds of thousands of tons, and the amount of culture medium req...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 张术臻刘希军
Owner QINGDAO CREAT MEDIUM
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