Lipase mutant with improved heat stability, and construction method thereof
A technology for thermostability and lipase, applied in the field of lipase mutants and their construction, can solve the problems of limited application scope, poor thermostability, restricting the development and application of lipase, etc., achieving broad market prospects and improving thermostability , the effect of high practical application value
Active Publication Date: 2012-09-12
SHANGHAI JIAO TONG UNIV
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Abstract
The invention provides a lipase mutant with improved heat stability and a construction method thereof. The lipase mutant with improved heat stability is obtained by subjecting Candida Antarctica lipase B gene to several rounds of fixed point saturation mutagenesis, the Candida Antarctica lipase B having an amino acid residue sequence expressed by SEQ ID No:1, and the lipase mutant having amino acid residue sequences expressed by SEQ ID No:2, SEQ ID No:3 or SEQ ID No:4, each of which consists of 317 amino acids. A method of semi-rational design is utilized in the invention, and a lipase mutantis obtained by subjecting Candida Antarctica lipase B gene to several rounds of fixed point saturation mutagenesis, wherein the mutant includes amino acid mutations D223G, L278M and combination of the two. Expressed by T50<15> and half-life t1/2 at the temperature of 48 DEG C respectively, the heat stability of the lipase mutant is improved, and a high actual application value and broad market prospects are also provided.
Application Domain
BacteriaHydrolases +3
Technology Topic
Amino acid compositionMutagenic Process +7
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