Composite microbial inoculum organic multielement compound fertilizer and production method thereof
A technology of compound bacterial agent and compound fertilizer, applied in fertilizer mixture, fertilization device, agriculture, etc., can solve the problem that fertilizer cannot meet the requirements of agricultural production
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Embodiment 1
[0088] Preparation of solid mixed bacterial agent:
[0089] Arthrobacter AS1.8; Culture of Alcaligenes AS1.448, 1.768:
[0090] Slant culture method: 5 parts of peptone, 3 parts of meat extract, 5 parts of sodium chloride and 15 parts of agar are dissolved in 1000 parts of distilled water in parts by weight to prepare a culture medium, which is divided into test tubes, packed to 1 / 2 the capacity of the test tube. Then it was sterilized under the condition of 1kg / cm2 pressure and 121°C for 30 minutes, after cooling, it was inoculated with Arthrobacter AS1.8, and then put into a constant temperature incubator and cultivated at 30-33°C for 48 hours. Likewise, Alcaligenes AS1.448 was cultured similarly.
[0091] Secondary seed expansion culture method: In order to speed up the growth rate of the bacterial count, the liquid expansion culture method is adopted here. The formula of the culture medium is the same as that of the slant plane of the bacteria, except that agar is not ad...
Embodiment 2
[0148] This embodiment is operated the same as Example 1, just using Bacillus megaterium ACCC10011, Thiobacillus thiooxidans AS1.1479, Arthrobacter AS1.8, Alcaligenes AS1.768, Bacillus colloidus ACCC10013, Bacillus circulans AS1. 553. Streptomyces flavinus, ACCC40027, Streptomyces jingyangensis ACCC40021; the above bacterial agents are mixed according to the weight ratio of 1, 9, 1, 21, 9, 9, 1, 49 to form a composite solid bacterial agent.
[0149] 0.77 kg of magnesium sulfate, 0.44 kg of manganese sulfate, 0.03 kg of ammonium molybdate, 0.15 kg of ferrous sulfate, 0.05 kg of copper sulfate, 0.1 kg of zinc sulfate, 0.26 kg of borax, 14 kg of potassium chloride, 9.2 kg of diammonium phosphate, 2 kg Urea and 30 kg of ammonium sulfate, 43 kg of organic materials containing bacterial agents, including 25 kg of composite solid bacterial agents.
[0150] The fertilizer is applied to different crops in different regions, and all achieve ideal results. Its effects can be summarized ...
Embodiment 3
[0157] This embodiment operates as in Example 1, but uses Bacillus megaterium ACCC10010, Thiobacillus thiooxidans AS1.1479, Arthrobacter AS1.8, Alcaligenes AS1.768, Bacillus coliform ACCC10014, Bacillus circulans AS1. 554. Streptomyces flavinus, AS4.407; Streptomyces jingyangensis ACCC40022; the above bacterial agents are mixed according to the weight ratio of 1, 9, 1, 21, 9, 9, 49, 1 to form a composite solid bacterial agent.
[0158] 0.56 dry grams of magnesium sulfate, 0.39 kilograms of manganese sulfate, 0.03 kilograms of ammonium molybdate, 0.12 kilograms of ferrous sulfate, 0.03 kilograms of copper sulfate, 0.11 kilograms of zinc sulfate, 0.26 kilograms of borax, 23 kilograms of potassium chloride, 18 kilograms of monoammonium phosphate, 24.5 Kilograms of urea; 33 kilograms of organic materials containing bacteria agents, including 5 kilograms of composite solid bacteria agents.
[0159] The fertilizer is applied to different crops in different regions, and all achieve i...
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