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Method for extracting DHA (docosahexaenoic acid) through extraction and separation of marine microalgae fermentation liquid

A technology for marine microalgae and fermentation broth, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of long extraction process route, low oil extraction rate, insufficient wall breaking, etc., and reduce the discharge of three wastes , the effect of improving the extraction rate and reducing the production cost

Active Publication Date: 2013-01-23
青岛科源海洋生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that the bacteria are first flocculated and then broken during the operation. Since the flocculants are organic or inorganic polymer substances, the bacteria in the fermentation broth will be aggregated and connected to form larger flocculent aggregates. , and attached to the surface of the bacteria to form a layer of coating, resulting in insufficient wall breaking during subsequent operations, resulting in a low oil extraction rate
The present invention uses a high-speed centrifuge with a rotating speed of 8000-12000 rpm to separate and directly obtain crude oil, and the refinement of crude oil adopts five-stage molecular distillation, which can effectively achieve the effects of decolorization, deacidification and deodorization, but the disadvantages of this technology are In the preparation process, it is necessary to break the wall of the biological enzyme first, and then carry out the five-stage molecular distillation, which makes the whole extraction process long, complicated, and high in energy consumption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) After introducing 1000g of Crypthecodinium cohnii fermentation broth (initial total lipid content is 90g) into the flocculation and sedimentation tank, add 10g of chitosan flocculant, collect the precipitated algae after 45 minutes of flocculation, and centrifuge Machine centrifugation, remove flocculant and water, obtain muddy algae, and then freeze-dry it to make microalgae dry powder with water content ≤ 5%;

[0026] (2) Add 400g of 6# organic solvent to the dry microalgae powder obtained in step (1) for leaching and extraction, the extraction temperature is 45°C, and the number of extractions is four times to obtain the extract;

[0027] (3) Concentrating the extract obtained in step (2) to remove the solvent, the vacuum degree is 0.4Kpa, and the removal time is 1 hour to obtain DHA crude oil;

[0028] (4) Add 50g of phosphoric acid solution with a mass percentage concentration of 85% and 200g of a NaCl solution with a mass percentage concentration of 30% to the...

Embodiment 2

[0031](1) After introducing 1000g of Ulkeniaamoeboida fermentation liquid (initial total lipid content is 103g) into the flocculation and sedimentation tank, add 20g of polyacrylamide flocculant, collect the precipitated algae after 60 minutes of flocculation, and centrifuge to separate , remove the flocculant and water to obtain muddy algae, and then freeze-dry it to make microalgae dry powder with a water content of ≤5%;

[0032] (2) Add 600g of acetone to the dry microalgae powder obtained in step (1) for leaching and extraction, the extraction temperature is 60°C, and the extraction times are twice to obtain the extract;

[0033] (3) Concentrate the extract obtained in step (2) to remove the solvent, the vacuum degree is 0.30Kpa, and the removal time is 5 hours to obtain DHA crude oil;

[0034] (4) Add 200g of phosphoric acid solution with a mass percentage concentration of 50% and 50g of NaCl solution with a mass percentage concentration of 70% to the crude oil obtained i...

Embodiment 3

[0037] (1) After introducing 1000g of Thraustochytrids fermentation broth (initial total lipid content is 98.3g) into the flocculation and sedimentation tank, add 15g of alum flocculant, collect the precipitated algae after 30 minutes of flocculation, and pass through a disc centrifuge Centrifuge to remove the flocculant and water to obtain muddy algae, and then freeze-dry it to make microalgae dry powder with a water content of ≤5%;

[0038] (2) Add 500 g of ethyl acetate to the dry microalgae powder obtained in step (1) for leaching and extraction, the extraction temperature is 50° C., and the number of extractions is three times to obtain an extract;

[0039] (3) Concentrating the extract obtained in step (2) to remove the solvent, the vacuum degree is 0.35Kpa, and the removal time is 3 hours to obtain DHA crude oil;

[0040] (4) Add 150g of phosphoric acid solution with a mass percent concentration of 65% and 100g of a NaCl solution with a mass percent concentration of 60%...

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Abstract

The invention relates to a method for extracting DHA (docosahexaenoic acid) through the extraction and separation of a marine microalgae fermentation liquid, which comprises the following steps: (1) dehydrating and drying a DHA microalgae fermentation liquid to obtain DHA algae powder; (2) selecting a low-boiling extraction agent to extract the DHA algae powder so as to obtain an extract liquid; (3) performing vacuum concentration on the extract liquid, and removing the solvent to obtain DHA raw oil; and (4) performing decolorization and vapor deodorization on the DHA raw oil, and then concentrating to obtain a DHA algae oil finished product. According to the invention, the total extraction yield of microalgae fermentation DHA production can be improved, the production cost can be lowered, and the discharge amount of three wastes can be decreased; and meanwhile, the extraction agent can be recovered and reutilized, thereby reducing environmental pollution.

Description

technical field [0001] The invention relates to a method for extracting microbial oil, in particular to a method for extracting and separating marine microalgae fermentation liquid to extract DHA. Background technique [0002] DHA, the full name of docosahexaenoic acid, commonly known as brain gold, is a polyunsaturated fatty acid that is very important to the human body, and is an important member of the Omega-3 unsaturated fatty acid family. DHA is a main element for the growth and maintenance of nervous system cells. It is an important component of the brain and retina. Its content in the human cerebral cortex is as high as 20%, and it accounts for the largest proportion in the retina of the eye, accounting for about 50%. Therefore, for Intellectual and visual development of the fetus and baby are crucial. [0003] Medical research shows that the main function of DHA can promote fetal brain development, promote the maturation of retinal photoreceptor cells, and promote t...

Claims

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Application Information

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IPC IPC(8): C07C57/03C07C51/42C07C51/47
Inventor 李悦明张希铭徐建春夏修峦
Owner 青岛科源海洋生物有限公司